Studies on Sex Determination with Different Y-Specific Probes After Quantifying Human Genomic DNA with the Slot Blot Hybridization Method
Sex determination can be performed routinely by Southern blot analysis, by the PC Reaction or by dot/slot blot analysis. In forensic science the method for sex identification has to be fast and the amount of DNA available is sometimes limited. In case of vaginal swabs additionaly a good separation of female and male cells after differential lysis is required. At first the extracted DNA from forensic specimen has to be quantified. One possibility is the slot blot hybridization method with a human specific probe. It represents a method to detect small amounts of degraded DNA and to test its human specifity . In this study a rehybridization of the membrane with different human Y-specific probes is investigated to identify the sex, to test human Y-specifity and to proof the quality of the differential lysis. Two alkaline phosphatase labelled human Y-specific probes are tested for their usefulness and one longer digoxigenin labelled human Y-specific probe. This method requires no additional DNA material for sex determination and quality control of DNA extracted from vaginal swabs.
KeywordsHybridization Result Vaginal Swab Slot Blot Slot Blot Hybridization Human Specifity
Unable to display preview. Download preview PDF.
- Easteal, S., McLeod, N.; Reed, K. (1991): DNA Profiling. Harwood Academic Publishers, Philadelphia, 152–153.Google Scholar