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Studies on Sex Determination with Different Y-Specific Probes After Quantifying Human Genomic DNA with the Slot Blot Hybridization Method

  • H. Haas
  • W. Hofmann
  • G. Weiler
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

Sex determination can be performed routinely by Southern blot analysis, by the PC Reaction or by dot/slot blot analysis. In forensic science the method for sex identification has to be fast and the amount of DNA available is sometimes limited. In case of vaginal swabs additionaly a good separation of female and male cells after differential lysis is required. At first the extracted DNA from forensic specimen has to be quantified. One possibility is the slot blot hybridization method with a human specific probe. It represents a method to detect small amounts of degraded DNA and to test its human specifity [1]. In this study a rehybridization of the membrane with different human Y-specific probes is investigated to identify the sex, to test human Y-specifity and to proof the quality of the differential lysis. Two alkaline phosphatase labelled human Y-specific probes are tested for their usefulness and one longer digoxigenin labelled human Y-specific probe. This method requires no additional DNA material for sex determination and quality control of DNA extracted from vaginal swabs.

Keywords

Hybridization Result Vaginal Swab Slot Blot Slot Blot Hybridization Human Specifity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature

  1. [1]
    Waye, J.S.; Presley, L.A.; Budowle, B.; Shutler, G.G.; Fourney, R.M. (1989): A Simple and Sensitive Method for Quantifying Human Genomic DNA in Forensic Specimen Extracts. BioTechniques 7 (8), 852–855.PubMedGoogle Scholar
  2. [2]
    Easteal, S., McLeod, N.; Reed, K. (1991): DNA Profiling. Harwood Academic Publishers, Philadelphia, 152–153.Google Scholar
  3. [3]
    Nakahori, Y.; Mitani, K.M.; Yamada, M.; Nakagome, Y. (1988): A Human Y-Chromosome Specific Repeated Family (DYZ1) Consists of a Tandem Array of Pentanucleotides. Nucl.Acids Res. 14, 7569–7580.CrossRefGoogle Scholar
  4. [4]
    Feinberg, A.P.; Vogelstein, B. (1983): A Technique for Radiolabelling DNA Restriction Endonuclease Fragments to High Specific Activity. Anal.Biochem. 132, 6–13.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • H. Haas
    • 1
  • W. Hofmann
    • 1
  • G. Weiler
    • 1
  1. 1.Institute of Legal MedicineUniversity of GiessenGiessenGermany

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