Orosomucoid Phenotyping Using Monoclonal Antibodies
Orosomucoid(ORM) is a major glycoprotein in human serum and shows extensive variation. About 30 alleles heve been described at ORM1 and ORM2 locus, respectively (Yuasa et al. 1993). The ORM1 and the ORM2 alloproteins are encoded by two closely linked genes on the long arm of the chromosome 9 (Dente et al. 1987) and are distinguishable in band intensity from each other (Yuasa et al. 1986). In the course of ORM phenotyping, we have occasionally encountered some bands which are difficult to determine whether they belong to ORM1 or ORM2 product. In this study to overcome such confusions we attempted to prepare locus-specific monoclonal antibodies.
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