Phenotype and Allele Frequencies of 4 VNTR-AMPFLP’s in an Austrian Population Sample
Microsatellite loci (VNTR, Variable Number of Tandem Repeats), a highly polymorphic system of non-coding marker loci dispersed throughout the whole human genome with constant rates were introduced to paternity and identity testing by Jeffreys et al (1988). Initially, typing has been carried out by Restriction Fragment Length Polymorphism (RFLP). Since this method has its limitaions and moreover, is time-consuming and difficult to perform and to analyze, typing by Amplification Fragment Length Polymorphism (AMPFLP, Budowle et al 1991) represents a major improvement to the typing of VNTR-loci. Since this is a simple and reliable technique and sufficently high exclusion chances can easily be achieved, it will presumably replace conventional systems for paternity and identity testing in the near future. Population and family studies that are necessary to select and to introduce new marker systems to forensic haemogenetics have already been performed by a number of workers(see Lit.). In this paper, we present the allele and phenotype frequencies of an Austrian population sample typed for the VNTR-loci ApoB, COL2A1, D1S80 and YNZ22.
KeywordsPhenotype Frequency Allele Assignment VNTR Locus Stickler Syndrome Amplification Fragment Length Polymorphism
Unable to display preview. Download preview PDF.
- Maerz W, Ruzicka V, Fischer E, Russ AP, Schneider W, Groß W (1993) Typing of the 3’hypervariable region of the apolipoprotein B gene: Approaches, pitfalls and applications. Electrophoresis 14: 169Google Scholar
- Sajantila A, Budowle B, Stroem M, Johnsson V, Lukka M, Peltonen L, Ehnholm C (1992) PCR Amplification of Alleles at the D1S80 Locus: Comparison of a Finnish and a North American Caucasian Population Sample, and Forensic Casework Evaluation. Am J Hum Genet 50: 816Google Scholar