High Resolution Analysis by PCR on an Automated DNA Sequencer of Internal Variation at a Pseudoautosomal VNTR (DXYS17)
PCR-based DNA typing has become a valid alternative for the time-consuming and laborious typing of VNTR loci by Southern blot analysis. It has not only increased the sensitivity of DNA typing to levels of a few nanogram of DNA but it allows also to have a higher resolution for small size differences. Genotyping becomes possible which is in contrast to conventional Southern blot methods where the low resolution for small size differences, sometimes, lead to ‘pseudo-homozygosity’ (1).
KeywordsUrea Agarose Bromide Electrophoresis Polyacrylamide
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