High Resolution Analysis by PCR on an Automated DNA Sequencer of Internal Variation at a Pseudoautosomal VNTR (DXYS17)

  • R. Decorte
  • P. Marynen
  • J.-J. Cassiman
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

PCR-based DNA typing has become a valid alternative for the time-consuming and laborious typing of VNTR loci by Southern blot analysis. It has not only increased the sensitivity of DNA typing to levels of a few nanogram of DNA but it allows also to have a higher resolution for small size differences. Genotyping becomes possible which is in contrast to conventional Southern blot methods where the low resolution for small size differences, sometimes, lead to ‘pseudo-homozygosity’ (1).

Keywords

Urea Agarose Bromide Electrophoresis Polyacrylamide 

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References

  1. 1.
    Chakraborty R, Fornage M, Gueguen R, Boerwinkle E. Population genetics of hypervariable loci: Analysis of PCR based VNTR polymorphism within a population. In: Burke T, Dolf G, Jeffreys AJ, Wolff R (eds) DNA Fingerprinting: Approaches and Applications, Birkhâuser Verlag Basel, pp 127–143 (1991)CrossRefGoogle Scholar
  2. 2.
    Decorte R, Cuppens H, Marynen P, Cassiman JJ. Rapid detection of hypervariable regions by the polymerase chain reaction technique. DNA Cell Biol 9: 461–469 (1990)PubMedCrossRefGoogle Scholar
  3. 3.
    Decorte R, Marynen P, Cassiman JJ. High-speed automated detection of PCR-amplified VNTR alleles: application to the apolipoprotein B 3’ hypervariable region. Science Tools 37:1–4 (1993)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • R. Decorte
    • 1
  • P. Marynen
    • 1
  • J.-J. Cassiman
    • 1
  1. 1.Center for Human GeneticsK.U.LeuvenLeuvenBelgium

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