Molecular Analysis of Esterase D Polymorphism
Since the polymorphism of Esterase D(EsD) was described initially by Hopkinson et al. (1973), a number of population studies were reported. EsD polymorphism has been one of the most useful markers in genetic studies. Three common phenotypes, EsD 1, EsD 2–1 and EsD 2, are identified and determined by two autosomal codominant alleles, EsD 1 and EsD 2. Although the complete EsD cDNA have been sequenced, there are no information about the phenotypes of EsD. In this study, we make clear the relationship between EsD isozyme phenotypes detected by electrophoresis and DNA sequences. Moreover, a RFLP related to EsD polymorphism is presented and applied to the EsD typing by DNA samples.
KeywordsPhenol Codon Chloroform Glycine Electrophoresis
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