Molecular Analysis of Esterase D Polymorphism

  • S. Tsuchida
  • E. Fukui
  • S. Ikemoto
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

Since the polymorphism of Esterase D(EsD) was described initially by Hopkinson et al. (1973), a number of population studies were reported. EsD polymorphism has been one of the most useful markers in genetic studies. Three common phenotypes, EsD 1, EsD 2–1 and EsD 2, are identified and determined by two autosomal codominant alleles, EsD 1 and EsD 2. Although the complete EsD cDNA have been sequenced, there are no information about the phenotypes of EsD. In this study, we make clear the relationship between EsD isozyme phenotypes detected by electrophoresis and DNA sequences. Moreover, a RFLP related to EsD polymorphism is presented and applied to the EsD typing by DNA samples.

Keywords

Phenol Codon Chloroform Glycine Electrophoresis 

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References

  1. Hopkinson DA, Mestriner MA, Cortner J, Harris H (1973) Esterase D, a new human polymorphism. Ann Hum Genet Lond. 37: 119–137CrossRefGoogle Scholar
  2. Maniatis T, Fritsch EF, Sambrook J (eds) (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar
  3. Young L-JS, Lee EY-HP, To H, Bookstein R, Shew J-Y, Donoso LA, Sery 1 Giblin M, Shields JA, Lee W-H (1988) Human esterase D gene: complete cDNA sequence, genomic structure, and application in the genetic diagnosis of human retinoblastoma. Hum Genet 79: 137–141PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • S. Tsuchida
    • 1
  • E. Fukui
    • 1
  • S. Ikemoto
    • 1
  1. 1.Department of Legal Medicine and Human GeneticsJichi Medical SchoolTochigiJapan

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