Identification of Human Remains Using Mitochondrial DNA Sequencing: Potential Mother-Child Mutational Events
Determining the primary sequence of DNA is the ultimate form of DNA testing for discriminating between individuals. Various DNA sequencing methodologies are currently available, although the dideoxynucleotide “terminator” chemistry of Sanger is the most widely used in laboratories today (1,2). The development of fluorescence and infrared-based detection methods, coupled with automated DNA sequencing instruments, has greatly improved throughput and efficiency (3–7). Therefore, sequencing technology is readily available to most laboratories, and automation has made the process more cost effective.
KeywordsHypervariable Region Sterile Deionized Water Human Remains Purine Nucleotide Pyrimidine Nucleotide
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