Identification of Human Remains Using Mitochondrial DNA Sequencing: Potential Mother-Child Mutational Events

  • M. M. Holland
  • R. K. Roby
  • D. L. Fisher
  • J. Ruderman
  • D. A. Lee
  • C. K. Bryson
  • T. Kupferschmid
  • R. S. Lofts
  • A. J. Eisenberg
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

Determining the primary sequence of DNA is the ultimate form of DNA testing for discriminating between individuals. Various DNA sequencing methodologies are currently available, although the dideoxynucleotide “terminator” chemistry of Sanger is the most widely used in laboratories today (1,2). The development of fluorescence and infrared-based detection methods, coupled with automated DNA sequencing instruments, has greatly improved throughput and efficiency (3–7). Therefore, sequencing technology is readily available to most laboratories, and automation has made the process more cost effective.

Keywords

Phenol Albumin EDTA Agarose Electrophoresis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Maxam, A. M. and Gilbert, W. (1977) Proc. Nat. Acad. Sci. USA, 74, 560–564.PubMedCrossRefGoogle Scholar
  2. 2.
    Sanger, F., Nicklen, S., and Coulson, A.R. (1977) Proc. Nat. Acad. Sci. USA 74, 5463–5467.PubMedCrossRefGoogle Scholar
  3. 3.
    Smith, L.M., Sanders, J.Z., Kaiser R.J., Hughes, P., Dodd, C., Connell, C.R., Heiner, C., Kent, S.B.H., and Hood, L.E. (1986) Nature 321, 674–679.PubMedCrossRefGoogle Scholar
  4. 4.
    Ansorge, W., Sproat, B., Stegemann, J., Schwager, C., and Zenke, M. (1987) Nuc. Acids Res. 15, 4593–4602.CrossRefGoogle Scholar
  5. 5.
    Connell, C., Fung, S., Heiner, C., Bridgeham, J., Chakerian, V., Heron, E., Jones, B., Menchen, S., Mordan, W., Raff, M., Recknor, M., Smith, L., Springer, J., Woo, S., and Hunkapiller, M. (1987) BioTechniques 5, 342–348.Google Scholar
  6. 6.
    Prober, J.M., Trainor, G.L., Dam, R.J., Hobbs, F.W., Robertson, C.W., Zagursky, R.S., Cocuzza, A.J., Jensen, M.A., and Baumeister, K. (1987) Science 238, 336–341.PubMedCrossRefGoogle Scholar
  7. 7.
    Brumbaugh, J.A., Middendorf, L.R., Grone, D.L., and Ruth J.L. (1988) Proc. Nat. Acad. Sci USA 85, 5610–5614.PubMedCrossRefGoogle Scholar
  8. 8.
    Sullivan, K.H., Hopgood, R., and Gill, P. (1992) Int. J. Legal Med. 105, 83–86.PubMedCrossRefGoogle Scholar
  9. 9.
    Ginther, C, Issel-Tarver, L., and King, M.-C. (1992) Nature Genomics 2, 135–138.CrossRefGoogle Scholar
  10. 10.
    Holland, M.M., Fisher, D.L., Mitchell, L.G., Rodriguez, W.C., Canik, J.J., Merril, C.R., and Weedn, V.W. (1993) J. For. Sci. 38, 542–553.Google Scholar
  11. 11.
    Holland, M.M., Fisher, D.L., Roby, R.K., Ruderman, J., Bryson, C., and Weedn, V.W. (1993) Mitochondrial DNA sequence analysis of human remains, Crime Laboratory Digest, submitted for publication May 1993.Google Scholar
  12. 12.
    Ivanov, P. (1993) DNA-based identification of the last Russia’s royal family remains, The Fourth International Symposium on Human Identification, Scottsdale, AZ, September 29, 1993.Google Scholar
  13. 13.
    Robin, E.D. and Wong, R. (1988) J. Cell. Phys., 136, 507–513.CrossRefGoogle Scholar
  14. 14.
    Giles, R.E., Blanc, H., Cann, H.M., and Wallace, D.C. (1980) Proc. Nat. Acad. Sci. USA, 77, 6715–6719.PubMedCrossRefGoogle Scholar
  15. 15.
    Aquadro, C.F. and Greenberg, B.D. (1983) Genetics, 103, 287–312.PubMedGoogle Scholar
  16. 16.
    Cann, R.L., Brown, W.M., and Wilson, A.C. (1984) Genetics, 106, 479–499.PubMedGoogle Scholar
  17. 17.
    Cann, R.L., Stoneking, M., and Wilson, A.C. (1987) Nature, 325, 31–36.PubMedCrossRefGoogle Scholar
  18. 18.
    Anderson, S., Bankier, A.T., Barrell, G.B., et al. (1981) Nature, 290, 457–465.PubMedCrossRefGoogle Scholar
  19. 19.
    Stoneking, M., Hedgecock, D., Higuchi, R.G., Vigilant, L., and Erlich, H.A. (1991) Am. J. Hum. Genet. 48, 370–382.PubMedGoogle Scholar
  20. 20.
    Pena, S. (1993) Mitochondrial D-loop “signatures” with low-stringency specific primer PCR (LSSP-PCR): A novel approach to identity testing, The Fourth International Symposium on Human Identification, Scottsdale, AZ, September 29, 1993.Google Scholar
  21. 21.
    Orrego, C. and King, M.-C. (1990) PCR Protocols - A Guide to Methods and Applications M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White (eds.), Academic Press, London, San Diego, pp. 416–426.Google Scholar
  22. 22.
    Sullivan, K. M., Hopgood, R., Lang, B., and Gill, P. (1991) Electrophoresis, 12, 1721.CrossRefGoogle Scholar
  23. 23.
    Walsh, P. S., Metzger, D. A., Higuchi, R. (1991) BioTechniques, 10, 506–513.PubMedGoogle Scholar
  24. 23.
    Walsh, P. S., Metzger, D. A., Higuchi, R. (1991) BioTechniques, 10, 506–513.PubMedGoogle Scholar
  25. 25.
    Dr. Charles L. Ginther, The University of California, Berkeley, Department of Biomedical and Environmental Health Sciences, School of Public Health, Berkeley, CA, personal communication.Google Scholar
  26. 26.
    Dr. Mark Stoneking, Department of Anthropology, Pennsylvania State University, University Park, PA, personal communication.Google Scholar
  27. 27.
    Dr. Kevin Sullivan, The Central Research and Support Establishment, The Home Office Forensic Science Service, England.Google Scholar
  28. 28.
    Holland, M. M., Roby, R. K., Ruderman, J., Fisher, D. L., Lee, D. A., Kupferschmid, T., Lofts, R. S., Eisenberg, A. V., A new perspective on the mutation rate of the human mitochondrial DNA genome, manuscript in preparation.Google Scholar
  29. 29.
    Budowle B., Chakraborty R., Giusti A. M., Eisenberg A. J., Allen R. C. (1991) Am. J. Hum. Genet., 48, 137–144.PubMedGoogle Scholar
  30. 30.
    Edwards A., Civitello A., Hammond H. A., Caskey C. T. (1991) Am. J. Hum. Genet. 49, 746–756.PubMedGoogle Scholar
  31. 31.
    Edwards A., Hammond H. A., Jin L., Caskey C. T., Chakraborty R. (1992) Genomics, 12, 241–253.PubMedCrossRefGoogle Scholar
  32. 32.
    Polymeropoulos M. H., Rath D. S., Xiao H., Merril C. R. (1992) Nuc. Acids. Res., 20, 1432.CrossRefGoogle Scholar
  33. 33.
    Holland, M. M., Fisher, D. L., Lee, D. A., Bryson, C. K., and Weedn, V. W. (1993) DNA Fingerprinting; State of the Science S. D. Pena, R. Chakraborty, J. T. Epplen, & A. J. Jeffreys (eds.), Birkhauser Verlag Basel, Switzerland, 267–274.CrossRefGoogle Scholar
  34. 34.
    Swerdlow, H., Zhang, J. Z., Chen, D. Y., Harke, H. R., Grey, R., Wu, S., and Dovichi, N. J. (1991) Anal. Chem., 63, 2835–2841.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • M. M. Holland
    • 1
  • R. K. Roby
    • 1
  • D. L. Fisher
    • 1
  • J. Ruderman
    • 1
  • D. A. Lee
    • 1
  • C. K. Bryson
    • 1
  • T. Kupferschmid
    • 1
  • R. S. Lofts
    • 1
  • A. J. Eisenberg
    • 2
  1. 1.The Armed Forces DNA Identification LaboratoryThe Armed Forces Institute of PathologyUSA
  2. 2.Department of PathologyUniversity of North Texas Health Science CenterFt. WorthUSA

Personalised recommendations