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Protein Patterns of Benign and Malignant Human Melanocytes Show Consistent Changes in Gene Expression

  • Conference paper
Skin Cancer: Basic Science, Clinical Research and Treatment

Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 139))

Abstract

For human malignant melanoma, no pattern of oncogene activation has yet been identified which consistently occurs in the malignant cells. In order to better understand the transformation process, we studied the overall gene expression at the protein level in human melanoma cells in vitro by two-dimensional gel electrophoresis, For that, four established cell lines, MEWO, M5, SKMEL13, and IGR39, were used and compared with newly established cultures of normal human melanocytes. Considerable variations in the protein patterns of the different melanoma cell lines were found, whereas the patterns of normal human melanocytes correlated fairly well with each other

With respect to the expression of single proteins, we identified four major proteins which were consistently found in cultured melanocytes and stringently repressed in the four melanoma cell lines examined. On the other hand, induction of new proteins in the different melanoma cell lines was found to be less stringent and also less uniform. We propose that malignant transformation of melanocytes may be more associated with the suppression of melanocytic proteins rather than with new expression of melanoma specific proteins

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Authors and Affiliations

  1. Department of Dermatology, The Free University of Berlin, University Medical Center, Steglitz, Hindenburgdamm 30, 12200, Berlin, Germany

    J. Eberle, C. Garbe, G. Kroumpouzos & C. E. Orfanos

Authors
  1. J. Eberle
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  2. C. Garbe
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  3. G. Kroumpouzos
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  4. C. E. Orfanos
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Editor information

Editors and Affiliations

  1. University Department of Dermatology Medical Centre Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200, Berlin, Germany

    Claus Garbe , Stefan Schmitz  & Constantin E. Orfanos ,  & 

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© 1995 Springer-Verlag Berlin · Heidelberg

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Eberle, J., Garbe, C., Kroumpouzos, G., Orfanos, C.E. (1995). Protein Patterns of Benign and Malignant Human Melanocytes Show Consistent Changes in Gene Expression. In: Garbe, C., Schmitz, S., Orfanos, C.E. (eds) Skin Cancer: Basic Science, Clinical Research and Treatment. Recent Results in Cancer Research, vol 139. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78771-3_9

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  • DOI: https://doi.org/10.1007/978-3-642-78771-3_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78773-7

  • Online ISBN: 978-3-642-78771-3

  • eBook Packages: Springer Book Archive

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