Abstract
A method for obtaining accurate, quantitative 57Fe Mössbauer spectra from biological samples is illustrated stepwise in a data reduction procedure. Exact criteria are presented for deciding when it is necessary to account for the effects of the Beer-Lambert law in the Mössbauer spectra from biological samples. This procedure makes extensive use of the fast Fourier transform and other computer techniques in its data reduction and its curve-fitting stages. A method for optimizing sample thickness is presented. The choice of truncation in Fourier space as a means to numerically stabilize the deconvolution procedure is defended. Several advantages for curve fitting in Fourier space are shown. Maximization of information content is discussed for Mossbauer spectral simulation techniques.
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© 1994 Federation of European Biochemical Societies
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Dunham, W.R., Harding, L.J., Sands, R.H. (1994). Mössbauer spectroscopy of metalloproteins and the use of Fourier transforms. In: EJB Reviews 1993. EJB Reviews, vol 1993. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78757-7_7
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DOI: https://doi.org/10.1007/978-3-642-78757-7_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-57620-4
Online ISBN: 978-3-642-78757-7
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