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Gene regulation in rodent hepatocytes during development, differentiation and disease

  • Kleanthis G. Xanthopoulos
  • Jovan Mirkovitch
Part of the EJB Reviews book series (EJB REVIEWS, volume 1993)

Abstract

The expression of genes in the liver is mostly controlled at the transcriptional level and depends on the regulatory interactions between cis-acting sequences and trans-acting molecules. Proximal promoters and distant enhancers in combination with a number of hepatocyte-enriched DNA-binding proteins and general transcription factors interact specifically with these elements and control the expression of liver-specific genes. Hepatocyte-enriched regulatory proteins have been isolated from liver nuclear extracts, characterized, and their corresponding genes have been cloned. These include the hepatocyte nuclear factors 1, 3, 4 (HNF-1,3,4), some members of the CAAAT/enhancer binding protein (C/EBP) family, and D site binding protein (DBP). These factors belong to larger families and are able to form heterodimers, perhaps with the exception of the HNF-3 family, with other members of the same family Interestingly, the majority of the genes encoding such proteins are themselves regulated at the transcriptional level, although both transcriptional and post-transcriptional events modulate their expression during development, hepatocyte differentiation and disease, suggesting that a transcriptional cascade may play a critical role in mammalian liver development and differentiation.

Keywords

Basal Transcription Factor Liver Gene Expression Forkhead Gene Liver Nuclear Extract Ubiquitous Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

HNF

hepatocyte nuclear factor

C/EBP

CAAAT/enhancer binding protein

NF-IL6

nuclear factor IL-6

IL6-DBP

IL-6 dependent binding protein

AGP/EBP

α 1-acid glycoprotein/enhancer binding protein

LAP

liver activator protein

CRP II

C/EBP related protein II

rNFIL-6

rat nuclear factor IL-6

DBP

D site binding protein

bZIP

basic leucine zipper

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Copyright information

© Federation of European Biochemical Societies 1994

Authors and Affiliations

  • Kleanthis G. Xanthopoulos
    • 1
  • Jovan Mirkovitch
    • 2
  1. 1.Karolinska Institute, Center for BiotechnologyNOVUMHuddingeSweden
  2. 2.Swiss Institute for Experimental Cancer ResearchÉpalinges-sur-LausanneSwitzerland

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