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The anatomy of a kinase and the control of phosphate transfer

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EJB Reviews 1993

Part of the book series: EJB Reviews ((EJB REVIEWS,volume 1993))

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Abstract

In this article we look in depth at the properties of a kinase, phosphoglycerate kinase (PGK). The object of the account is to seek common features of controlled phosphate transfer reactions not only in kinases, but also in ATP-utilising pumps and in ATP synthases found in bio-energy capture devices (Table 1). We started work in this area using PGK as a model for the F, section of ATP synthase [1]. The value of PGK today in this model study rests on the following features. (a) It is of sufficient size to demonstrate both phosphate transfer and the control mechanisms for that transfer. (b) Crystal structures are available for three enzymes, each in at least two different states, both in the presence and absence of substrates. (c) Proton NMR spectroscopy studies have been used to examine the protein and many of its mutants, both structure and dynamics, in several states in the presence and absence of substrates. (d) There is a wealth of kinetic data on the enzyme and on mutants. These studies allow us to describe the dynamics of the reaction which can then be used in an effort to appreciate a wide range of kinetic analyses of phosphate transfer reactions.

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Abbreviations

PGK:

phosphoglycerate kinase

Gri(1,3)P 2 :

1,3-bisphosphoglycerate

Gri3P :

3-phosphoglycerate

H388Q etc.:

mutant in which His388 has been replaced by Gln, etc.

References

  1. Williams R. J. P. (1975) in Electron transfer chains and oxidative phosphorylation (E. Quogliarello, ed.) North-Holland, Amsterdam.

    Google Scholar 

  2. Watson, H. C. & Littlechild, J. A. (1990) Biochem. Soc. Trans. 18, 187–190.

    PubMed  CAS  Google Scholar 

  3. Fifis, T. & Scopes, R. K. (1978) Biochem. J. 175, 311–319.

    PubMed  CAS  Google Scholar 

  4. Bryant, T. N., Watson, H. C. & Wendell, P. L. (1974) Nature 247, 14–17.

    Article  PubMed  CAS  Google Scholar 

  5. Watson, H. C., Walker, N. P. C., Shaw, P. J., Bryant, T. N., Wendell, P. L., Fothergill, L. A., Perkins, R. E., Conroy, S. C., Dobson, M. J., Tuite, M. F., Kingsman, A. J. & Kingsman, S. M. (1982) EMBO J. 1, 1635–1640.

    PubMed  CAS  Google Scholar 

  6. Blake, C. C. E & Evans, P. R. (1974) J. Mol. Biol. 84, 585–601.

    Article  PubMed  CAS  Google Scholar 

  7. Banks, R. D., Blake, C. C. F., Evans, P. R., Haser, R., Rice, D. W., Hardy, G. W., Merrett, M. & Phillips, A. W. (1979) Nature 279, 773–777.

    Article  PubMed  CAS  Google Scholar 

  8. Blake, C. C. F. & Rice, D. W. (1981) Phil. Trans. R. Soc. Lond. A. 293, 93–104.

    Article  CAS  Google Scholar 

  9. Michelson, A. M., Markham, A. F. & Orkin, S. H. (1983) Proc. Natl Acad. Sci. USA 80, 472–476.

    Article  PubMed  CAS  Google Scholar 

  10. Osinga, K. A., Swinkels, B. W., Gibson, W. C., Borst, P., Veeneman, G. H., van Boom, J. H., Michels, P. A. M. & Opperdoes, F. R. (1985) EMBO J. 4, 3811–3817.

    PubMed  CAS  Google Scholar 

  11. Mori, N., Singer-Sam, J. & Riggs, A. D. (1986) FEBS Lett. 204, 313–317.

    Article  PubMed  CAS  Google Scholar 

  12. Clements, J. M. & Roberts, C. F. (1986) Gene 44, 97–105.

    Article  PubMed  CAS  Google Scholar 

  13. Bowen, D., Littlechild, J. A., Fothergill, J. E., Watson, H. C. & Hall, L. (1988) Biochem. J. 254, 509–517.

    PubMed  CAS  Google Scholar 

  14. Conway, T. & Ingram, L. O. (1988) J. Bacteriol. 170, 1926–1933.

    PubMed  CAS  Google Scholar 

  15. Swinkels, B. W., Evers, R. & Borst, P. (1988) EMBO J. 7, 1159–1165.

    PubMed  CAS  Google Scholar 

  16. Davies, G. D., Gamblin, S. J., Littlechild, J. A. &Watson, H. C. (1993) Proteins, in the press.

    Google Scholar 

  17. Blake, C. C. F., Rice, D. W. & Cohen, F. E. (1986) Int. J. Rep. Protein Res. 27, 443–448.

    Article  CAS  Google Scholar 

  18. Tanswell, P., Westhead, E. W. & Williams, R. J. P. (1974) FEBS Lett. 48, 60–63.

    Article  PubMed  CAS  Google Scholar 

  19. Tanswell, P., Westhead, E. W. & Williams, R. J. P. (1976) Eur. J. Biochem. 63, 249–258.

    Article  PubMed  CAS  Google Scholar 

  20. Fairbrother, W. J. (1989) D. Phil. Thesis, University of Oxford.

    Google Scholar 

  21. Graham, H. C. (1991) D. Phil. Thesis, University of Oxford.

    Google Scholar 

  22. Graham, H. C. & Williams, R. J. P. (1991) Eur. J. Biochem. 197, 81–91.

    Article  PubMed  CAS  Google Scholar 

  23. Minard, P., Hall, L., Betton, J.-M., Missiakas, D. & Yon, J. M. (1989) Protein Eng. 3, 55–60.

    Article  PubMed  CAS  Google Scholar 

  24. Fairbrother, W. J., Minard, R, Hall, L., Betton, J.-M., Missiakas, D., Yon, J. M. & Williams R. J. R. (1989) Protein Eng. 3, 511.

    Article  Google Scholar 

  25. Burgess, R. J. & Pain, R. H. (1977) Biochem. Soc. Trans. 5, 692–694.

    PubMed  CAS  Google Scholar 

  26. Adams, B., Burgess, R. J., Carrey, E. A., Mackintosh, I. R., Mitchinson, C., Thomas, R. M. & Pain, R. H. (1980) in Protein folding (Jaenicke, R., ed.) Elsevier/North-Holland Biomedical Press, Amsterdam.

    Google Scholar 

  27. Adams, B., Burgess, R. J. & Pain R. H. (1985) Eur. J. Biochem. 152, 715–720.

    Article  PubMed  CAS  Google Scholar 

  28. Betton, J., Desmadril, M. & Yon, M. J. (1989) Biochemistry 28, 5421–5428.

    Article  PubMed  CAS  Google Scholar 

  29. Fairbrother, W. J., Walker, R. A., Minard, P., Littlechild, J. A., Watson, H. C. & Williams, R. J. R. (1989) Eur. J. Biochem. 183, 57–67.

    Article  PubMed  CAS  Google Scholar 

  30. Wilson, H. R., Williams, R. J. P., Littlechild, J. A. & Watson, H. C. (1988) Eue. J. Biochem. 170, 529–538.

    Article  CAS  Google Scholar 

  31. Fairbrother, W. J., Graham, H. C. & Williams, R. J. P. (1990) Eur. J. Biochem. 190, 407–414.

    Article  PubMed  CAS  Google Scholar 

  32. Nagasuma, R. & Watson, H. C. (1992) Eur. J. Biochem. 205, 1077–1088.

    Article  PubMed  Google Scholar 

  33. de Vos, A. M., Tong, L., Milburn, M. V., Matias, P. M., Jancarik, J., Noguchi, S., Nishimura, S., Miura, K., Ohtsuka, E. & Kim, S.-H. (1988) Science 239, 888–893.

    Article  PubMed  Google Scholar 

  34. Harlos, K., Vas, M. & Blake, C. C. F. (1992) Proteins 12, 133–144.

    Article  PubMed  CAS  Google Scholar 

  35. Webb, M. R. & Trentham, D. R. (1980) J. Biol. Chem. 255, 1775–1778.

    PubMed  CAS  Google Scholar 

  36. Fairbrother, W. J., Graham, H. C. & Williams, R. J. P. (1990) Biochem. 190, 161–169.

    CAS  Google Scholar 

  37. Dalgarno, D., Drabikowski, W., Klevit, R., Levine, B. A., Scott, M. & Williams, R. J. P. (1983) in Calcium, binding proteins (de Bernard, B., ed.) pp. 83–91, Elsevier, Amsterdam.

    Google Scholar 

  38. Chothia, C., Lesk, A. M, Dobson, G. G. & Hodgkins, D. C. (1983) Nature 302, 500–505.

    Article  PubMed  CAS  Google Scholar 

  39. Lesk, A. M. & Chothia, C. (1984) J. Mol. Biol. 174, 175–191.

    Article  PubMed  CAS  Google Scholar 

  40. Larsson-Rainikiewicz, M. & Malmström, B. G. (1961) Arch. Biochem. Biophys. 92, 94–99.

    Article  Google Scholar 

  41. Larsson-Rainikiewicz, M. (1964) Biochim. Biophys. Acta 85, 60–68.

    Google Scholar 

  42. Larsson-Raznikiewicz, M. (1967) Biochim Biophys. Acta 132, 33–40.

    PubMed  CAS  Google Scholar 

  43. Larsson-Raznikiewicz, M. (1970) Eur. J. Biochem. 15, 574–580.

    Article  PubMed  CAS  Google Scholar 

  44. Larsson-Raznikiewicz, M. & Arvidsson, L. (1971) Eue. J. Biochem. 22, 506–512.

    Article  CAS  Google Scholar 

  45. Larsson-Raznikiewicz, M. & Jansson, J. R. (1973) FEBS Lett. 29, 345–347.

    Article  PubMed  CAS  Google Scholar 

  46. Larsson-Rainikiewicz, M. & Schierbeck, D. (1974) Biochim. Biophys. Acta 481, 283–287.

    Google Scholar 

  47. Schierbeck, B. & Larsson-Raznikiewicz, M. (1979) Biochim. Biophys. Acta 568, 195–204.

    PubMed  CAS  Google Scholar 

  48. Khamis, M. M. & Larsson-Raznikiewicz, M. (1981) Biochim. Biophys. Acta 657, 190–194.

    PubMed  CAS  Google Scholar 

  49. Ali, M. & Brownstone, Y. S. (1976) Biochim Biophys. Acta 445, 89–103.

    PubMed  CAS  Google Scholar 

  50. Beissner, R. S. & Rudolph F. B. (1979) J. Biol. Chem. 254, 6273–6277.

    PubMed  CAS  Google Scholar 

  51. Orr, G. A. & Knowles, J. R. (1974) Biochem. J. 141, 721–723.

    PubMed  CAS  Google Scholar 

  52. Scopes, R. K. (1978) Eur. J. Biochem. 91, 119–129.

    Article  PubMed  CAS  Google Scholar 

  53. Scopes, R. K. (1978) Eur. J. Biochem. 85, 503–516.

    Article  PubMed  CAS  Google Scholar 

  54. Walker, P. A., Joao, H. C., Littlechild, J. A., Williams, R. J. P. & Watson, H. C. (1992) Eue. J. Biochem. 207, 29–37.

    Article  CAS  Google Scholar 

  55. Sherman, M. A., Szipkowska, B. K., Dean, S. A., Mathiowetz, A. M., McQueen, N. L. & Mas, M. T. (1990) J. Biochem. 265, 10659–10665.

    CAS  Google Scholar 

  56. Fairbrother, W. J., Hall, L., Littlechild, J. A., Walker, R. A., Watson, H. C. & Williams, R. J. R (1989) FEBS Lett. 258, 247–250.

    Article  PubMed  CAS  Google Scholar 

  57. Graham, H. C., Williams, R. J. R, Littlechild, J. A. & Watson, H. C. (1991) Eue J. Biochem. 196, 261–269.

    Article  CAS  Google Scholar 

  58. Joao, H. C., Taddei, N. & Williams, R. J. R (1992) Eue J. Biochem. 205, 93–104.

    Article  CAS  Google Scholar 

  59. Minard, R, Bowen, D. J., Hall, L., Littlechild, J. A. & Watson, H. C. (1990) Protein Eng. 3, 515–521.

    Article  PubMed  CAS  Google Scholar 

  60. Fersht, A. R., Shi, J. R, Knill-Jones, J., Lowe, D M., Wilkinson, A. J., Blow, D. M., Carter, P., Waye, M. M. Y. & Winter, G. (1985) Nature 14, 235–238.

    Article  Google Scholar 

  61. van Solingen, P., Muurling, H., Koekman, B. & van den Berg, J. (1988) Nucleic Acid Res. 16, 11823.

    Article  PubMed  Google Scholar 

  62. Wilson, C. A. B., Hardman, N., Fothergill-Gilmore, L. A., Gamblin, S. R. & Watson, H. C. (1987) Biochem. J. 241, 609–614.

    PubMed  CAS  Google Scholar 

  63. Walker, P. A., Littlechild, J. A., Hall, L. & Watson, H. C. (1989). Eur. J. Biochem. 183, 49–55.

    Article  PubMed  CAS  Google Scholar 

  64. Mas, M. T., Resplandor, Z. E. & Riggs, A. D. (1987) Biochemistry 26, 5369–5377.

    Article  PubMed  CAS  Google Scholar 

  65. Mas, M. T., Bailey, J. M. & Resplandor, Z. E. (1988) Biochemistry 27, 1168–1172.

    Article  PubMed  CAS  Google Scholar 

  66. Adams, S. R., Sparkes, M. J. & Dixon, H. B. F. (1983) Biochem. J. 213, 211–215.

    PubMed  CAS  Google Scholar 

  67. Kamiya, K., Cruse, W. B. T. & Kennard, O. (1983) Biochem. J. 213, 217–223.

    PubMed  CAS  Google Scholar 

  68. Pickover, C. A., McKay, D. B., Engelman, D. M. & Steitz, T. A. (1979) J. Biol. Chem. 254, 11323–11329.

    PubMed  CAS  Google Scholar 

  69. Ptisyn, R B., Pavlov, M. Y., Sinev, M. A. & Timchenko, A. A. (1986) in Multidomain proteins (Patthy, L. & Friedrich, P., eds) pp. 9–25, Akademiai Kiado, Budapest.

    Google Scholar 

  70. Sachsenheimer, W. & Schulz, G. E. (1977) J. Mol. Biol. 114, 23–36.

    Article  PubMed  CAS  Google Scholar 

  71. Levine, B. A., Dalgarno, D. C., Esnouf, M. R, Klevit, R. E., Scott, G. M. M. & Williams, R. J. R. (1983) Ciba Found. Symp. 93, 72–90.

    PubMed  CAS  Google Scholar 

  72. Baldwin, J. & Chothia, C. J. (1979) J. Mol. Biol. 129, 175–220.

    Article  PubMed  CAS  Google Scholar 

  73. Schulz, G. E., Bidermann, W., Kabsch, R. & Schirmer, H. (1973) Ciba Found. Symp. 80, 857–864.

    CAS  Google Scholar 

  74. Steitz, T. A., Fletterick, R. J. & Hwang, K. J. (1973) Ciba Found. Symp. 78, 551–561.

    CAS  Google Scholar 

  75. Fletterick, R. J., Bates, D. N. & Steitz, T. A. (1975) Proc. Natl Acad. Sci. USA 72, 38–42.

    Article  PubMed  CAS  Google Scholar 

  76. Buttlaire, D. H. & Cohn, M. (1974) J. Biol. Chem. 249, 5733–5740.

    PubMed  CAS  Google Scholar 

  77. McDonald, R., Steitz, T. A. & Engelman, D. M. (1979) Biochemistry 18, 338–342.

    Article  PubMed  CAS  Google Scholar 

  78. Peters, B. A. & Neet, K. E. (1978) J. Biol. Chem. 253, 68–28.

    Google Scholar 

  79. Müller, K., Kratky, O., Röschlau, R. & Hess, B. (1972) HoppeSeyler’s Z. Physiol. Chem. 353, 803.

    Article  Google Scholar 

  80. Evans, R R. & Hudson, P. J. (1972) Proc. FEBS Mat. 79, 4349.

    Google Scholar 

  81. Wierenga, R. K., Terpstra, R. & Hol, W. G. J. (1986) J. Mol. Biol. 187, 101–107.

    Article  PubMed  CAS  Google Scholar 

  82. Taylor, S. S. (1987) BioEssays 7, 24–29.

    Article  PubMed  CAS  Google Scholar 

  83. Taylor, S. S. (1989) J. Biol. Chem. 264, 8443–8446.

    PubMed  CAS  Google Scholar 

  84. Knighton, D. R., Zheng, J., Eyck, L. F. T., Ashford, V. A., Xuang, N.-H., Taylor, S. S. & Sowadski, J. M. (1991) Science 253, 407–414.

    Article  PubMed  CAS  Google Scholar 

  85. Saraste, M., Sibbald, P. R. & Wittinghofer, A. (1990) Trends Biochem. Sci. 15, 430–434.

    Article  PubMed  Google Scholar 

  86. Schultz, G. E., Schiltz, E., Tomasselli, A. G., Frank, R., Brune, M., Wittinghofer, A. & Schirmer, R. H. (1986) Eur. J. Biochem. 161, 127–132.

    Article  Google Scholar 

  87. Anderson, C. M., Zucker, F. H. & Steitz, T. A. (1979) Science 204, 375–380.

    Article  PubMed  CAS  Google Scholar 

  88. Flaherty, K. M., DeLucca-Flaherty, C. & McKay, D. B. (1990) Nature 346, 623–628.

    Article  PubMed  CAS  Google Scholar 

  89. Kabasch, W., Mannherz, H. G., Suck, D., Fai, E. F. & Holmes, K. C. (1990) Nature 347, 37–44.

    Article  Google Scholar 

  90. Taylor, S. S., Ringheim, G. E., Neitzel, J. J., Yonemoto, W. M., Sowadski, J. M. & Dospmann, W. (1988) Cold Spring Harbor Symp. Quant. Biol. 53, 121–130.

    PubMed  CAS  Google Scholar 

  91. Boyer, P. D. (1993) Biochim Biophys. Acta 1140, 215–250.

    Article  PubMed  CAS  Google Scholar 

  92. Cohen, P. & Klee, C. B. (eds) (1988) Calmodulin, Elsevier, Amsterdam.

    Google Scholar 

  93. Kim, J. & Rees, D. C. (1992) Science 257,1653–1659.

    Article  Google Scholar 

  94. Wang, K. K. W., Villalobo, A. & Roufogalis, B. D. (1992) Trends Cell Biol. 2,46–52.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Inorganic Chemistry Laboratory, University of Oxford, England

    Heidi C. João

  2. Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, England, OX1 3QR

    Robert J. P. Williams

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© 1994 Federation of European Biochemical Societies

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João, H.C., Williams, R.J.P. (1994). The anatomy of a kinase and the control of phosphate transfer. In: EJB Reviews 1993. EJB Reviews, vol 1993. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78757-7_11

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  • DOI: https://doi.org/10.1007/978-3-642-78757-7_11

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