Abstract
The glycoprotein hormones lutropin (LH), follitropin (FSH), thyrotropin (TSH), and chorionic gonadotropin (CG) are dimeric proteins. They consist of a common a subunit and highly homologous, but hormone-specific, β subunits (Fig. 1). Even though the structures of the glycoprotein hormones are closely related at the primary, secondary, and tertiary levels, the structures of their Asn-linked oligosaccharides differ. LH and TSH bear oligosaccharides which terminate with the unique sequence SO44GalNAcβ1, 4GlcNAcβ1, 2Manα (S4GGnM) whereas FSH and CG bear oligosaccharides which terminate with the sequence sialic acid α2, 3/6Galβ1, 4GlcNAcβ1, 2Manα (Baenziger and Green 1991; Green and Baenziger 1988a, b; Green et al. 1985b; Endo et al. 1979; Mizuochi and Kobata 1980). The synthesis of these sulfated oligosaccharides reflects the activity of two highly specific enzymes, a GalNAc-transferase and a sulfotransferase.
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References
Baenziger JU, Kumar S, Brodbeck RM, Smith PL, Beranek MC (1992) Circulatory half-life but not interaction with the lutropin/chorionic gonadotropin receptor is modulated by sulfation of bovine lutropin in oligosaccharides. Proc Natl Acad Sci USA 89:334–338
Baenziger JU, Green ED (1991) Structure, synthesis, and function of the asparagine-linked oligosaccharides on pituitary glycoprotein hormones. In: Ginsberg B, Robbins PW (eds) Biolgoy of carbohydrates, vol 3. JAI Press Ltd, London, pp 1–46
Dharmesh S, Skelton TP, Baenziger JU (1993) Co-ordinate and restricted expression of the PXR/K-specific GalNAc-transferase and the GalNAcβl, 4GlcNAcβl, 2Manα-4-sulfotrans-ferase. (unpubl)
Dharmseh SM, Baenziger JU (1993) Co-ordinate regulation of lutropin, GalNAc- and sulfo-transferase in pituitary, (unpubl)
Endo Y, Yamashita K, Tachibana Y, Tojo S, Kobata A (1979) Structures of the Asparagine-linked sugar chains of human chrionic gonadotropin. J Biochem (Tokyo) 85:669–679
Fiete D, Srivastava V, Hindsgaul O, Baenziger JU (1991) A hepatic reticuloendothelial cell receptor specific for SO4-4GalNAcβl, 4GlcNAcβi, 2Manα that mediates rapid clearance of lutropin. Cell 67:1103–1110
Green ED, Morishima C, Boime I, Baenziger JU (1985a) Structural requirements for sulfation of asparagine-linked oligosaccharides of lutropin. Proc Natl Acad Sci USA 82:7850–7854
Green ED, Van Halbeek H, Boime I, Baenziger JU (1985b) Structural elucidation of the di-sulfated oligosaccharide from bovine lutropin. J Biol Chem 260:15623–15630
Green ED, Baenziger JU (1988a) Asparagine-linked oligosaccharides on lutropin, follitropin, and thryotropin: I. Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones. J Biol Chem 263:25–35
Green ED, Baenziger JU (1988b) Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin: II. Distributions of sulfated and sialylated oligosaccharides on bovine, ovine, and human glycoprotein hormones. J Biol Chem 263:36–44
Keel BA (1989) In: Keel BA, Grotjan HE ( Microheterogeneity of glycoprotein hormones. CRC Press Boca Raton, pp 203–215
Mengeling BJ, Smith PL, Stults NL, Smith DF, Baenziger JU (1991) A microplate assay for analysis of solution phase glycosyltransferase reactions: determination of kinetic constants. Anal Biochem 199:286–292
Mizuochi T, Kobata A (1980) Different asparagine-linked sugar chains on the two polypeptide chains of human chorionic gonadotropin. Biochem Biophys Res Commun 97:772–778
Rodriguez MC, Xie Y-B, Wang H, Collison K, Segaloff DL (1992) Effects of truncations of the cytoplasmic tail of the luteinizing hormone/chorionic gonadotropin receptor on receptor-mediated hormone internalization. Mol Endocrinol 6:327–336
Skelton TP, Hooper LV, Srivastava V, Hindsgaul O, Baenziger JU (1991) Characterization of a sulfotransferase responsible for the 4-O-sulfation of terminal β-N-acetyl-D-galactosamine on asparagine-linked oligosaccharides of glycoprotein hormones. J Biol Chem 266:17142–17150
Skelton TP, Kumar S, Smith PL, Beranek MC, Baenziger JU (1992) Proopiomelanocortin synthesized by corticotrophs bears asparagine-linked oligosaccharides terminating with SO4-4GalNAcβl, 4GlcNAcβl, 2Manα. J Biol Chem 267:12998–13006
Smith PL, Kaetzel D, Nilson J, Baenziger JU (1990) The sialylated oligosaccharides of recombinant bovine lutropin modulate hormone bioactivity. J Biol Chem 265:874–881
Smith PL, Skelton TP, Fiete D, Dharmesh SM, Beranek MC, MacPhail L, Broze GJ, Jr., Baenziger JU (1992) The asparagine-linked oligosaccharides on tissue factor pathway inhibitor terminate with SO4-4GalNAcβl, 4GlcNAcβl, 2Manα. J Biol Chem 267:19140–19146
Smith PL, Bousfield GS, Kumar S, Fiete D, Baenziger JU (1993) Equine lutropin and chorionic gonadotropin bear oligosaccharides terminating with SO4-4-GalNAc and sialic acid α2, 3Gal respectively. J Biol Chem 268:795–802
Smith PL, Baenziger JU (1988) A pituitary N-acetylgalactosamine transferase that specifically recognizes glycoprotein hormones. Science 242:930–933
Smith PL, Baenziger JU (1990) Recognition by the glycoprotein hormone-specific N-acetyl-galactosaminetransferase is independent of hormone native conformation. Proc Natl Acad Sci USA 87:7275–7279
Smith PL, Baenziger JU (1992) Molecular basis of recognition by the glycoprotein hormon-specific N-acetylgalactosamine-transferase. Proc Natl Acad Sci USA 89:329–333
Veldhuis JD, Evans WS, Johnson ML, Wills MR, Rogol AD (1986) Physiological properties of the luteinizing hormone pulse signal: impact of intensive and extended venous sampling paradigms on its characterization in healthy men and women. J Clin Endocrinol Metab 62:881–891
Veldhuis JD, Carlson ML, Johnson ML (1987) The pituitary gland secretes in bursts: appraising the nature of glandular secretory impulses by simultaneous multiple-parameter decon-volution of plasma hormone concentrations. Proc Natl Acad Sci USA 84:7686–7690
Veldhuis JD, Johnson ML (1988) A novel general biophysical model for simulating episodic endocrine gland signaling. Am J Physiol 255:E749-E759
Wang H, Segaloff DL, Ascoli M (1991) Lutropin/choriogonadotropin downregulates its receptor by both receptor-mediated endocytosis and a cAMP-dependent reduction in receptor mRNA. J Biol Chem 266:780–785
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© 1994 Springer-Verlag Berlin Heidelberg
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Baenziger, J.U. (1994). The Biologic Significance of Glycoprotein Hormone Oligosaccharides. In: Wieland, F., Reutter, W. (eds) Glyco-and Cellbiology. Colloquium der Gesellschaft für Biologische Chemie 22.–24. April 1993 in Mosbach/Baden, vol 44. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78729-4_15
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DOI: https://doi.org/10.1007/978-3-642-78729-4_15
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