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Baker-Williams Fractionation

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Abstract

The precipitation chromatography method suggested by Baker and Williams [1] is the combination of gradient elution (cf. Sect. 7) with an antiparallel temperature gradient [2–5]. Supports coated with the polymer are the same as in gradient elution, but only 5 to 10% of the support may be loaded with polymer. This support fraction is arranged at the hottest part of the column, i.e., usually at the top (when the eluent works in the normal-flow direction). The rest of the support is available for the chromatographic process. The solvent/nonsolvent gradient operates in the same way as in gradient elution.

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References

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© 1994 Springer-Verlag Berlin Heidelberg

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Francuskiewicz, F. (1994). Baker-Williams Fractionation. In: Polymer Fractionation. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78704-1_8

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  • DOI: https://doi.org/10.1007/978-3-642-78704-1_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78706-5

  • Online ISBN: 978-3-642-78704-1

  • eBook Packages: Springer Book Archive

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