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Interphasenzytogenetik mittels Fluoreszenz- In-situ-Hybridisierung (FISH)

  • J. Drach

Zusammenfassung

Molekularbiologische und zytogenetische Analysen haben in den vergangenen Jahren bedeutende Einblicke in die Biologie humaner Neoplasien erbracht, so daß heute die Entstehung und Progression von Tumoren mit spezifischen genetischen Verände rungen korreliert werden können. Dazu zählen insbesondere die t(9;22) bei der chronisch myeloischen Leukämie, die t(15;17) bei der akuten Promyelozytenleukämie, die t(14;18) bei follikulären Lymphomen, sowie Aktivierung und/oder Amplifizierung von Onkogenen (z. B. ras, c-myc, erbB-2) und Deletion von Tumor-Suppressor-Genen (p53, Rb-1), v.a. bei soliden Tumoren. Erste Informationen über diese Ereignisse lieferte die Karyotypisierung von Metaphasen-Präparationen maligner Zellen (konventionelle Zytogenetik). Die Tatsache, daß diese Technik nur an Zellen angewandt werden kann, welche sich zu Mitosen anregen lassen, stellt gleichzeitig eine der wichtigsten Limitationen dieser Methode dar.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

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  • J. Drach

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