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Durchflußzytometrie

  • W. Hilbe
  • W. Eisterer
  • H. Huber
  • J. Thaler

Zusammenfassung

Die Durchflußzytometrie in Verbindung mit leukozyten-spezifischen fluoreszenzmarkierten monoklonalen Antikörpern hat sich in den vergangenen Jahren als sehr effiziente Technik in der analytischen Zytologie manifestiert [63]. Gerade mit Hilfe der monoklonalen Antikörper ist eine Charakterisierung der hämatopoetischen Zellen in alien Entwicklungsstufen möglich. Für die Klassifizierung von Lymphomen und Leukämien stellt die Durchflußzytometrie eine sehr verläßliche Methode dar. In Zusammenschau mit Klinik und Morphologie kann meist auch in den schwierigsten Fällen eine definitive Diagnose gestellt werden. Mit Hilfe krankheitspezifischer Antikörperpanel kann weiters die Therapieeffizienz kontrolliert und beginnende Rezidive raschest erkannt werden.

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Literatur

  1. 1.
    Adriaansen, HJ, van Dongen, JJM, Kappers-Klunne, MC, Hählen, K, van’t Veer, MB, Wijdenes-de Bresser, JHFM, Holdrinet, ACJM, Harthoorn-Lasthuizen, EJ, Abels, J, Hooijkaas, H (1990) Terminal deoxynucleotidyl transferase positive subpopulations occur in the majority of ANLL: Implications for the detection of minimal disease. Leukemia 4:404–410PubMedGoogle Scholar
  2. 2.
    Bardales, RH, Carrato, A, Fleischer, M, Schwartz, MK, Koziner, B (1989) Detection of terminal deoxynucleotidyl transferase (TdT) by flow cytometry in leukemic disorders. J Histochem Cytochem 37:509–513PubMedCrossRefGoogle Scholar
  3. 3.
    Barlogie, B, Drewinko, B, Schumann, J, et al (1980) Cellular DNA content as a marker of neoplasia in man. Am J Med 69:195–203PubMedCrossRefGoogle Scholar
  4. 4.
    Bettelheim, P, Lutz, D, Majdic, O, Paietta, E, Haas, O, Linkesch, W, Neumann, E, Knapp, W (1985) Cell lineage heterogeneity in blast crisis of chronic myeloid leukemia. Br J Haematol 59:395PubMedCrossRefGoogle Scholar
  5. 5.
    Blanc, C, Legac, E, Binet, JL, Michel, A, Debré, P, Merle-Beral, H (1991) Characterization of normal human bone marrow by flow cytometry. Nouv Rev Fr Hematol 33:231–235PubMedGoogle Scholar
  6. 6.
    Boyd, AW, Anderson, KC, Freedman, AS, Fisher, DC, Slaughenhoupt, B, Schlossman, SF, Nadler, LM (1985) Studies of in vitro activation and differentiation of human B lymphocytes: I. Phenotypic and functional characterization of the B cell population responding to anti-Ig antibody. J Immunol 134:1516–1523PubMedGoogle Scholar
  7. 7.
    Bradstock, KF, Kirk, J, Grimsley, PG, Kabral, A, Hughes, WG (1989) Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations. Br J Haematol 72:512–518PubMedCrossRefGoogle Scholar
  8. 8.
    Campana, D, Coustan-Smith, E, Janossy, G (1990) The immunologic detection of minimal residual disease in acute leukemia. Blood 76:163–171PubMedGoogle Scholar
  9. 9.
    Chaudhary, PM, Roninson, IB (1991) Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells. Cell 66:85–94PubMedCrossRefGoogle Scholar
  10. 10.
    Clark, R, Peters, S, Hoy, T (1986) Prognostic importance of hypodiploid hemopoietic precursors in myelodysplastic syndromes. N Engl J Med 314:1472–1475PubMedCrossRefGoogle Scholar
  11. 11.
    Dekaris, D, Handl, S, Sabioncello, A, Rabatic, S (1990) Function of granulocytes in B-chronic lymphatic leukaemia. Blut 61:354–357PubMedCrossRefGoogle Scholar
  12. 12.
    Delwel, R, Touw, I, Löwenberg, B (1986) Towards detection of minimal disease: Discrimination of AML precursors from normal myeloid precursors using a combination of surface markers. In: Hagenbeck, A, Löwenberg, B (eds) Minimal Residual Disease in Acute Leukemia. Dordrecht: Martinus Nijhoff, 68–75CrossRefGoogle Scholar
  13. 13.
    Drach, J, Drach, D, Glassl, H, Gattringer, C, Huber, H (1992) Flow cytometric determination of atypical antigen in acute leukemia for the study of minimal residual disease. Cytometry 13:893–901PubMedCrossRefGoogle Scholar
  14. 14.
    Drach, J, Gattringer, C, Glassl, H, Schwarting, R, Stein, H, Huber, H (1989) Simultaneous flow cytometric analysis of surface markers and nuclear Ki-67 antigen in leukemia and lymphoma. Cytometry 10:743–749PubMedCrossRefGoogle Scholar
  15. 15.
    Drexler, HG, Gignac, SM, Minowada, J (1988) Routine immunophenotyping of acute leukemias. Blut 5:327–339CrossRefGoogle Scholar
  16. 16.
    Edwards, BS, Altobelli, KK, Nolla, HA, Harper, DA, Hoffmann, RR (1989) Comprehensive quality assessment approach for flow cytometric immunophenotyping of human lymphocytes. Cytometry 10:433–441PubMedCrossRefGoogle Scholar
  17. 17.
    Foon, KA, Todd, RF, III (1986) Immunologic classification of leukemia and lymphoma. Blood 68:1–31PubMedGoogle Scholar
  18. 18.
    Formenti, SC, Turner, RR, De Martini, RM, Boone, DC, Bishop, PC, Levine, AM, Parker, JW (1989) Immunophenotypic analysis of peripheral blood leukocytes at different stages of HIV infection: An analysis of asymptomatic, ARC, and AIDS populations. Am J Clin Pathol 92:300–307PubMedGoogle Scholar
  19. 19.
    Gahmberg, CG, Jokinen, M, Andersson, LC (1978) Expression of the major sialoglycoprotein (glycophorin) on erythroid cells in human bone marrow. Blood 52:379–387PubMedGoogle Scholar
  20. 20.
    Gerdes, J, Dallenbach, F, Lennert, K, Lemke, H, Stein, H (1984) Growth fractions in malignant non-Hodgkin’s lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol 2:365–371PubMedGoogle Scholar
  21. 21.
    Gore, SD, Kasten, MB, Civin, CI (1989) Normal human bone marrow cells which express CD 34 and terminal deoxynucleotidyl transferase (TdT) include T-cells and putative lymphoid stem cells. Blood 74:9 aGoogle Scholar
  22. 22.
    Griffin, JD, Todd, RF, Rith, J, Nadler, LM, Canellos, GP, Rosenthal, D, Callivan, M, Berveridge, RP, Weinstein, H, Karp, D, Schlossmann, SF (1983) Differentiation patterns in the blastic phase of chronic myeloid leukemia. Blood 61:85–91PubMedGoogle Scholar
  23. 23.
    Gucalp, R, Paietta, E, Weinberg, V, Papenhausen, P, Dutcher, JP, Wiernik, PH (1991) Terminal transferase expression in acute myeloid leukaemia: biology and prognosis. Br J Haematol 77:48–54CrossRefGoogle Scholar
  24. 24.
    Hasui, M, Hirabayashi, Y, Hattori, K, Kobayshi, Y (1991) Increased phagocytic activity of polymorphonuclear leukocytes of chronic granulomatous disease as determined with flow cytometric assay. J Lab Clin Med 117:291–298PubMedGoogle Scholar
  25. 25.
    Hollander, Z, Shah, VO, Civin, CI, Loken, MR (1988) Assessment of proliferation during maturation of the B lymphoid lineage in normal human bone marrow. Blood 71:528–531PubMedGoogle Scholar
  26. 26.
    Janossy, G, Coustan-Smith, E, Campana, D (1989) The reliability of cytoplasmatic CD 3 and CD 22 antigen expression in the immunodiagnosis of acute leukaemia: a study of 500 cases. Leukemia 3:170–181PubMedGoogle Scholar
  27. 27.
    Kaplan, SS, Penchansky, L, Stole, V, Contis, L, Krause, JR (1989) Immunophenotyping in the classification of acute leukemia in adults. Cancer 63:1520–1527PubMedCrossRefGoogle Scholar
  28. 28.
    Krishan, A, Sauerteig, A, Stein, JH (1991) Comparison of three commercially available antibodies for flow cytometric monitoring of P-glycoprotein expression in tumor cells. Cytometry 12:731–742PubMedCrossRefGoogle Scholar
  29. 29.
    Lai, RB, Edison, LJ, Chused, TM (1988) Fixation and long-term storage of human lymphocytes for surface marker analysis by flow cytometry. Cytometry 9:213–219CrossRefGoogle Scholar
  30. 30.
    Larsen, JK (1993) Cell proliferation: analysis by flow cytometry. Nouv Rev Fr Hematol 34:317–335Google Scholar
  31. 31.
    Le Bien, TW, Wormann, B, Villablanca, JD, Law, LM, Steinber, LM, Shah, VO, Loken, MR (1990) Multiparameter flow cytometric analysis of human fetal bone marrow B cells. Leukemia 4:354Google Scholar
  32. 32.
    Lebman, D, Trucco, M, Bottero, L, Lange, B, Pessano, S, Rovera, G (1982) A monoclonal antibody that detects expression of transferin receptor in human erythroid precursor cells. Blood 59:671PubMedGoogle Scholar
  33. 33.
    Liu, C, Muirhead, KA, George, SP, Landay, AL (1989) Flow cytometric monitoring of human immunodeficiency virus-infected patients. Am J Clin Pathol 92:721–728PubMedGoogle Scholar
  34. 34.
    Loken, MR, Civin, CI, Shah, VO, Fackler, MJ, Segers-Nolten, I, Terstappen, LWMM (1992) Characterization of erythroid, lymphoid and monomyeloid lineages in normal human bone marrow. In: Laerum, OD, Bjerknes, R (eds) Flow cytometry in hematology. Hartcourt Brace Jovanovich, London, 31–42Google Scholar
  35. 35.
    Loken, MR, Shah, VO, Dattilio, KL, Civin, CI (1987) Flow cytometric analysis of human bone marrow: Normal erythroid development. Blood 69:255–263PubMedGoogle Scholar
  36. 36.
    Loken, MR, Shah, VO, Dattilio, KL, Civin, CI (1987) Flow cytometric analysis of human bone marrow: II. Normal B lymphocyte development. Blood 70:1316–1324PubMedGoogle Scholar
  37. 37.
    Ludescher, C, Hilbe, W, Eisterer, W, Preuß, E, Gottwald, M, Hofmann, J, Thaler, J (1993) Activity of P-glycoprotein in B-cell chronic lymphocytic leukemia determined by a flow cytometric assay. J Natl Cancer Inst 85:1751–1758PubMedCrossRefGoogle Scholar
  38. 38.
    Ludescher, C, Hilbe, W, Eisterer, W, Preuß, E, Hofmann, J, Thaler, J (1994) Funktionelle Erfassung MDR-exprimierender Zellen mittels DurchfluBzytometrie. Wiener Klinische Wochenschrift 106/8:242–246Google Scholar
  39. 39.
    Ludescher, C, Thaler, J, Drach, D, Drach, J, Spitaler, M, Gattringer, C, Huber, H, Hofmann, J (1992) Detection of activity of P-glycoprotein in human tumour samples using rhodamine 123. Br J Haematol 82:161–168PubMedCrossRefGoogle Scholar
  40. 40.
    Ludwig, WD, Bartram, CR, Garbott, J, Köller, U, Haas, OA, Hansen-Hagge, T, Heil, G, Seibt-Jung, H, Teichmann, JV, Ritter, J, Knapp, W, Gadner, H, Thiel, E, Riehm, H (1989) Phenotypic and genotypic heterogeneity in infant acute leukemia. I. Acute lymphoblastic leukemia. Leukemia 3:431–439PubMedGoogle Scholar
  41. 41.
    Mahoney, JF, Urakaze, M, Hall, S, DeGasperi, R, Chang, H, Sugiyama, E, Warren, CD, Borowitz, M, Nicholson-Weller, A, Rosse, WF, Yeh, TH (1992) Defective glycosylphosphatidylinositol anchor synthesis in paroxysmal nocturnal hemoglobinuria granulocytes. Blood 79:1400–1403PubMedGoogle Scholar
  42. 42.
    Merkel, D, Dressier, L, McGuire, W (1987) Flow cytometry, cellular DNA content, and prognosis in human malignancy. J Clin Oncol 5:1690–1703PubMedGoogle Scholar
  43. 43.
    Nadler, LM, Korsmyer, SJ, Anderson, KC, Boyd, AW, Slaughenhoupt, B, Park, E, Jensen, J, Coral, F, Mayer, RJ, Sallen, SE, Ritz, J, Schlossman SF (1984) B cell origin of non-T cell acute lymphoblastic leukemia. J Clin Invest 74:332–340PubMedCrossRefGoogle Scholar
  44. 44.
    Neame, PB, Soamboonsrup, P, Browman, GP, Meyer, RM, Benger, A, Wilson, WEC, Walker, IR, Saeed, N, McBride, JA (1986) Classifying acute leukemia by immunophenotyping: a combined FAB-immunologic classification of AML. Blood 68:1355–1362PubMedGoogle Scholar
  45. 45.
    Pastan, I, Gottesman, M (1987) Multiple-drug resistance in human cancer. N Engl J Med 316:1388–1393PubMedCrossRefGoogle Scholar
  46. 46.
    Picker, L, Terstappen, LWMM, Roth, L, Streeter, P, Butcher, E (1990) Differential expression of homing-associated adhesion molecules by T-cell subsets in man. J Immunol 145:3247PubMedGoogle Scholar
  47. 47.
    Pollice, AA, McCoy, JP, Shackney, SE, Smith, CA, Agarwal, J, Burholt, DR, Janocko, LE, Hornicek, FJ, Singh, SG, Hartsock, RJ (1992) Sequential paraformaldehyde and methanol fixation for simultaneous flow cytometric analysis of DNA, cell surface proteins, and intracellular proteins. Cytometry 13:432–444PubMedCrossRefGoogle Scholar
  48. 48.
    Powell, BL, Olbrantz, P, Bicket, D, Bass, DA (1986) Altered oxidative burst product formation in neutrophils of patients recovering from therapy for acute leukemia. Blood 6:1624–1630Google Scholar
  49. 49.
    Reddy, MM, Grieco, MH (1991) Quantitative changes in T helper inducer (CD4+ CD45RA-), T suppressor inducer (CD4+ CD45RA+), T suppressor (CD8+ CDllb+), and T cytotoxic (CD8+ CDllb-) subsets in human immunodeficiency virus infection. J Clin Lab Anal 5:96–100PubMedCrossRefGoogle Scholar
  50. 50.
    Robinson, FJ, Sieff, C, Deilia, D, Edwards, PAW, Greaves, M (1981) Expression of cell surface HLA-DR, HLA-ABC and glycophorin during erythroid differentiation. Nature 289:68–71PubMedCrossRefGoogle Scholar
  51. 51.
    Rothe, G, Emmendorffer, A, Oser, A, Roesler, J, Valet, G (1991) Flow cytometric measurement of the respiratory burst activity of phagocytes using dihydrorhodamine 123. J Immunol Methods 138:133–135PubMedCrossRefGoogle Scholar
  52. 52.
    Rothe, G, Valet, G (1988) Phagocytosis intracellular pH, and cell volume in the multifunctional analysis of granulocytes by flow cytometry. Cytometry 9:316–324PubMedCrossRefGoogle Scholar
  53. 53.
    Rothe, G, Valet, G (1990) Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2’, 7’-dichlorofluorescein. J Leukocyte Biol 47:440–448PubMedGoogle Scholar
  54. 54.
    Ryan, D, Kossover, S, Mitchel, S, Frantz, C, Hennessy, L, Cohen, H (1986) Subpopulations of common acute lymphoblastic leukemia antigen positive lymphoid cells in normal bone marrow identified by hematopoietic differentiation antigens. Blood 68:417–425PubMedGoogle Scholar
  55. 55.
    Said, JW, Shintaku, IP, Pinkus, GS (1988) Immunohistochemical staining for terminal deoxynucleotidyl transferase (TDT). Am J Clin Pathol 89:649–652PubMedGoogle Scholar
  56. 56.
    Schubert, J, Alvarado, M, Uciechowski, P, Zielinska-Skowronek, M, Freund, M, Vogt, H, Schmidt, RE (1991) Diagnosis of paroxysmal nocturnal haemoglobinuria using immunophenotyping of peripheral blood cells. Br J Haematol 79:487–492PubMedCrossRefGoogle Scholar
  57. 57.
    Schwarting, R, Gerdes, J, Niehus, J, Jaeschke, L, Stein, H (1986) Determination of the growth fraction in cell suspensions by flow cytometry using the monoclonal antibody Ki-67. J Immunol Methods 90:65–70PubMedCrossRefGoogle Scholar
  58. 58.
    Schwarzinger, I, Valent, P, Köller, U, Marosi, C, Schneider, B, Haas, O, Lechner, K, Bettelheim, P (1990) Prognostic significance of surface marker expression on blast of patients with de novo AML. J Clin Oncol 8:423–430PubMedGoogle Scholar
  59. 59.
    Shackney, SE (1986) The use of flow cytometry in the diagnosis and biological characterization of the non-Hodgkin’s lymphomas. Ann NY Acad Sci 468:170–177CrossRefGoogle Scholar
  60. 60.
    Shah, VO, Safford, MG, Terstappen, LWMM, Loken, MR (1990) Quantitative comparison of myeloid antigens on peripheral blood lymphocytes, monocytes, neutrophils, eosinophils, and basophils. In: Knapp, W, Dorken, B, Gilks, WR, Riber, EP, Schmidt, RE, Stein, H, von dem Borne, AEGKr (eds) “Leucocyte Typing IV White Cell Differentiation Antigens”. Oxford University Press, 855–858.Google Scholar
  61. 61.
    Slaper-Cortenbach, ICM, Admiraal, LG, Kerr, JM, van Leeuwen, EF, von dem Borne, AEGKr, Tetteroo, PAT (1988) Flow-cytometric detection of terminal deoxynucleotidyl transferase and other intracellular antigens in combination with membrane antigens in acute lymphatic leukemias. Blood 72:1639–1644PubMedGoogle Scholar
  62. 62.
    Smith, RG, Kitchens, RL (1989) Phenotypic heterogeneity of TDT+cells in the blood and bone marrow: implications for surveillance of residual leukemia. Blood 74:312–319PubMedGoogle Scholar
  63. 63.
    Stewart, CC (1992) Clinical application of flow cytometry. Cancer 69:1543–1552PubMedCrossRefGoogle Scholar
  64. 64.
    Storr, J, Dolan, G, Coustan-Smith, E, Barnett, D, Reilly, JT (1990) Value of monoclonal antimyeloperoxidase (MP07) for diagnosing acute leukaemia. J Clin Pathol 43:847–849PubMedCrossRefGoogle Scholar
  65. 65.
    Terstappen, LWMM, Huang, S, Picker, LJ (1992) Flow cytometric assessment of human T-cell differentiation in thymus and bone marrow. Blood 79:666–677PubMedGoogle Scholar
  66. 66.
    Terstappen, LWMM, Johnsen, S, Segers-Nolten, I, Loken, MR (1990) Identification and characterization of normal human plasma cells by high resolution flow cytometry. Blood 76:1739–1747PubMedGoogle Scholar
  67. 67.
    Terstappen, LWMM, Loken, MR (1990) Myeloid cell differentiation in normal bone marrow and acute myeloid leukemia assessed by multidimensional flow cytometry. Anal Cell Pathol 2:229–240PubMedGoogle Scholar
  68. 68.
    Terstappen, LWMM, Safford, M, Koenemann, S, Loken, MR, Zurlutter, K, Buechner, T, Hiddemann, W, Woermann, B (1991) Flow cytometric characterization od acute myeloid leukemia. Part II. Phenotypic heterogeneity at diagnosis. Leukemia 5:757–767Google Scholar
  69. 69.
    Terstappen, LWMM, Safford, M, Loken, MR (1990) Flow cytometric analysis of human bone marrow: III. Neutrophil development. Leukemia 4:657–663PubMedGoogle Scholar
  70. 70.
    Van der Schoot, C, Daams, GM, Pinkster, J, Vet, R, von dem Borne, AEGKr (1990) Monoclonal antibodies against myeloperoxidase are valuable immunological reagents for the diagnosis of acute myeloid leukaemia. Br J Haematol 74:173–178PubMedCrossRefGoogle Scholar
  71. 71.
    Van der Schoot, CE, Daams, GM, Pinkster, J, Vet, R, von dem Borne, AEGK (1990) Monoclonal antibodies against myeloperoxidase are valuable immunological reagents for the diagnosis of acute myeloid leukaemia. Br J Haematol 74:173–178PubMedCrossRefGoogle Scholar
  72. 72.
    Vindelov, LL, Christensen, IJ, Jensen, G, et al (1983) Limits od detection of nuclear DNA abnormalities by flow cytometric DNA analysis. Cytometry 3:332–339PubMedCrossRefGoogle Scholar
  73. 73.
    Vindelov, LL, Christensen, IJ, Nissen, NI (1983) A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 3:323–327PubMedCrossRefGoogle Scholar
  74. 74.
    Wiersma, SR, Ortega, J, Sobel, E, Weinberg, KI (1991) Clinical importance of myeloid-antigen expression in acute lymphoblastic leukemia of childhood. N Engl J Med 324:800–808PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • W. Hilbe
  • W. Eisterer
  • H. Huber
  • J. Thaler

There are no affiliations available

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