Advertisement

Immunzytologie und Knochenmarkimmunhistologie

  • W. Eisterer
  • W. Hilbe
  • H. Huber
  • J. Thaler

Zusammenfassung

Die immunzytologische Phänotypisierung mit monoklonalen Antikörpern gestattet die eindeutige Charakterisierung von Zellen durch das Erstellen ihres antigenetischen Profils. Die Durchflußzytometrie (s. Kapitel XI), die Immunzytologie und die Immunhistologie stellen auf diesem Gebiet die grundlegenden Arbeitsmethoden dar.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. 1.
    Bartl, R, Frisch, B, Burkhardt, R (1984) Die Knochenmarkbiopsie. Karger, Basel, München, Paris, London, New York, Tokyo, Sydney 1984, 9–15Google Scholar
  2. 2.
    Bartl, R, Frisch, B, Burkhardt, R, Kettner, G, Mahl, G, Fareh-Moghadam, A, Sund, M (1982) Assessment of bone marrow histology in the malignant lymphomas (non-Hodgkin’s): correlation with clinical factors for diagnosis, prognosis, classification and staging. Br J Haematol 51:511–530PubMedCrossRefGoogle Scholar
  3. 3.
    Batge, B, Bosslet, K, Sedlacek, HH, Kern, HF, Kloppel, G (1986) Monoclonal antibodies against CEA-related components discriminate between pancreatic duct type carcinomas and nonneoplastic duct lesions as well as non duct type neoplasias. Virchows Archiv A Pathol Anat Histopathol 408:361–374CrossRefGoogle Scholar
  4. 4.
    Bennett, JM, Catovsky, D, Daniel, MT, Flandrin, G, Galton, DAG, Gralnick, HR, Sultan, C (1976) Proposals for the classification of the acute leukaemias; French-American-British (FAB) cooperative group. Br J Haematol 33:451–458PubMedCrossRefGoogle Scholar
  5. 5.
    Bosslet, K, Kern, HF, Kanzy, EJ, Steinstrasser, A, Schwarz, A, Luben, G, Schorlemmer, HU, Sedlacek, HH (1986) A monoclonal antibody with binding and inhibiting activity towards human pancreatic carcinoma cells. I. Immunhistological and immunochemical characterization of a murine monoclonal antibody selecting for well differentiated adenocarcinomas of the pancreas. Cancer Immunol Immunother 23:185–191PubMedCrossRefGoogle Scholar
  6. 6.
    Burkhardt, R, Frisch, B, Bartl, R (1982) Bone biopsy in haematological disorders. J Clin Pathol 35:257–284PubMedCrossRefGoogle Scholar
  7. 7.
    Casey, TT, Olson, SJ, Cousar, JB, Collins, RD (1989) Immunophenotypes of Reed-Sternberg cells: a study of 19 cases of Hodgkin’s disease in plastic-embedded sections. Blood 74: 2624–2628PubMedGoogle Scholar
  8. 8.
    Chilosi, M, Pizzolo, G, Fiore-Donati, L, Bofill, M, Janossy, G (1983) Routine immunofluorescent and histochemical analysis of bone marrow involvement of lymphoma/leukaemia: the use of cryostat sections. Br J Cancer 48:763–775PubMedCrossRefGoogle Scholar
  9. 9.
    Clark, JR, Williams, ME, Swerdlow, SH (1990) Detection of Band T-cells in paraffin-embedded tissue sections. Am J Clin Pathol 93:58–69PubMedGoogle Scholar
  10. 10.
    Davey, FR, Gatter, KC, Ralfkiaer, E, Pulford, KAF, Krissansen, GW, Mason, DY (1987) Immunophenotyping of non-Hodgkin’s lymphomas using a panel of antibodies on paraffinembedded tissues. Am J Pathol 129:54–63PubMedGoogle Scholar
  11. 11.
    De Waele, M, De Mey, J, Renmans, W, Labeur, C, Jochmans, K, Van Camp, B (1986) Potential of immunogold-silver staining for the study of leukocyte subpopulations as defined by monoclonal antibodies. J Histochem Cytochem 34:1257–1263PubMedCrossRefGoogle Scholar
  12. 12.
    De Waele, M, De Mey, J, Reynart, P, Dehou, MF, Gepts, W, Van Camp, B (1986) Detection of cell surface antigens in cryostat sections with immunogold-silver staining. Am J Clin Pathol 85:573–578PubMedGoogle Scholar
  13. 13.
    Eisterer, W (1993) Bedeutung der Knochenmarksimmunhistologie für die Prognose und Therapie der Chronisch-Lymphatischen Leukämie und des Lymphoplasmozytischen/Lymphoplasmozytoiden Immunozytoms (Dissertation). InnsbruckGoogle Scholar
  14. 14.
    Eisterer, W, Hilbe, W, Ludescher, C, Fend, F, Thaler, J (1993) Diagnostic value of immunophenotyping in low grade B-cell lymphoma. Br J Haematol (in press)Google Scholar
  15. 15.
    Erber, WN, Mynheer, LC, Mason, DY (1986) APAAP labeling of blood and bone-marrow samples for phenotyping leukaemia. Lancet 761–765Google Scholar
  16. 16.
    Falini, B, Martelli, MF, Tarallo, F, Moir, DJ, Cordell, JL, Gatter, KC, Loreti, G, Stein, H, Mason, DY (1984) Immunohistological analysis of human bone marrow trephine biopsies using monoclonal antibodies. Br J Haematol 56:365–386PubMedCrossRefGoogle Scholar
  17. 17.
    Feller, AC, Wacker, HH, Moldenhauer, G, Radzun, HJ, Parwaresch, MR (1987) Monoclonal antibody Ki-B3 detects a formalin resistant antigen on normal and neoplastic B cells. Blood 70:629–636PubMedGoogle Scholar
  18. 18.
    Gerdes, J, Dallenbach, F, Lennert, K, Lembke, H, Stein, H (1984) Growth fractions in malignant non-Hodgkin’s lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol 2:365–371PubMedGoogle Scholar
  19. 19.
    Hansmann, ML, Wacker, HH, Gralla, J, Lumbeck, H, Kossmahl, M, Heidebrecht, HJ, Radzun, HJ, Parwaresch, MR (1991) Ki-B5: a monoclonal antibody unrelated to CD45 recognizes normal and neoplastic human B cells in routine paraffin sections. Blood 15:809–817Google Scholar
  20. 20.
    Hsu, SM, Raine, L, Fanger, H (1981) Use of avidin-biotinperoxidase complex (ABC) in immunperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580PubMedCrossRefGoogle Scholar
  21. 21.
    Huber, H, Gattringer, C, Thaler, J, Peschel, Ch (1985) Immunzytologische Diagnose von Lymphomen und Leukämien: Monoclonale Antikörper in der Differentialdiagnose hämatologischer Neoplasien. Behring Inst Mitt 78:83–117PubMedGoogle Scholar
  22. 22.
    Huber, H, Löffler, H, Pastner, D (1992) Diagnostische Hämatologie. 3rd ed. Springer, Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong, Budapest, Barcelona 1992, 391–441CrossRefGoogle Scholar
  23. 23.
    Kawauchi, K, Sato, Y, Furuya, T, Watanabe, S (1989) Determination of proliferative fractions detected by immunohistochemistry with the monoclonal antibodies bromodeoxyuridine and Ki-67 in malignant lymphomas. Hematol Oncol 7:257–265PubMedCrossRefGoogle Scholar
  24. 24.
    Knapp, W, Rieber, P, Dörken, B, Schmidt, RE, Stein, H, Van den Borne, AEGK (1989) Towards a better definition of human leucocyte surface molecules. Immunol Today 10:253–259PubMedCrossRefGoogle Scholar
  25. 25.
    Lennert, K, Feller, AC (1990) Histopathologic der Non-Hodgkin-Lymphome (nach der aktualisierten Kiel-Klassifikation), 2nd ed. Springer Verlag, BerlinGoogle Scholar
  26. 26.
    Makin, CA, Bobrow, LG, Bodmer, WF (1984) Monoclonal antibody to cytokeratin for use in routine histopathology. J Clin Pathol 37:957–983Google Scholar
  27. 27.
    Marie, JP, Zittoun, R, Sikic, BI (1991) Multidrug resistance (MDR1) gene expression in adult acute leukemias: correlations with treatment outcome and in vitro drug sensitivity. Blood 78:586–592PubMedGoogle Scholar
  28. 28.
    Mason, DY, Gatter, KC (1987) The role of immunocytochemistry in diagnostic pathology. J Clin Pathol 40:1042–1054PubMedCrossRefGoogle Scholar
  29. 29.
    Navone, R, Colombano, MT (1984) Histopathological trephine biopsy findings in cases of “dry tap” bone marrow aspirations. Appl Pathol 2:264–271PubMedGoogle Scholar
  30. 30.
    Norton, AJ, Isaacson, PG (1987) The immunocytochemical diagnosis of malignant lymphoma in routinely processed tissues. J Pathol 151:240–241PubMedCrossRefGoogle Scholar
  31. 31.
    Oppedal, BR, Brandtzaeg, P, Kemshead, JT (1987) Immunohistochemical differentiation of neuroblastomas from other small round cell neoplasmas of childhood using a panel of monoand polyclonal antibodies. Histopathol 11:363–374CrossRefGoogle Scholar
  32. 32.
    Pastan, I, Gottesman, M (1987) Multiple-drug resistance in human cancer. N Engl J Med 316:1388–1393PubMedCrossRefGoogle Scholar
  33. 33.
    Schwarting, R, Stein, H, Wang, CY (1985) The monoclonal antibodies α-S-HCL 1 (αLeu-14) and α-S-HCLL3 (αLeu-M5) allow the diagnosis of hairy cell leukemia. Blood 65:974–983PubMedGoogle Scholar
  34. 34.
    Stein, H, Bonk, A, Tolksdorf, G, Lennert, K, Rodt, H, Gerdes, J (1980) Immunohistologic analysis of the organization of normal lymphoid tissue and non-Hodgkin’s lymphomas. J Histochem Cytochem 28:746–760PubMedCrossRefGoogle Scholar
  35. 35.
    Thaler, J, Dietze, O, Denz, H, Nachbaur, D, Stauder, R, Huber, H (1991) Bone marrow diagnosis in lymphoproliferative disorders: comparison of results obtained from conventional histomorphology and immunohistology. Histopathology 18:495–504PubMedCrossRefGoogle Scholar
  36. 36.
    Thaler, J, Dietze, O, Faber, V, Greil, R, Gastl, G, Denz, H, Ho, AD, Huber, H (1990) Monoclonal antibody B-ly7: a sensitive marker for detection of minimal residual disease in hairy cell leukemia. Leukemia 4:170–176PubMedGoogle Scholar
  37. 37.
    Thaler, J, Fechner, F, Herold, M, Huber, H (1993) Interleukin-6 in Multiple Myeloma: correlation with disease activity and Ki-67 proliferation index. Leukemia and Lymphoma (in press)Google Scholar
  38. 38.
    Thaler, J, Greil, R, Dietze, O, Huber, H (1989) Immunohistology for quantification of normal bone marrow lymphocyte subsets. Br J Haematol 73:576–577PubMedCrossRefGoogle Scholar
  39. 39.
    Thaler, J, Grünewald, K, Gattringer, C, Ho, AD, Weyrer, K, Dietze, O, Stauder, R, Fluckinger, T, Lang, A, Huber, H (1993) Long-term follow-up of patients with hairy cell leukaemia treated with pentostatin: lymphocyte subpopulations and residual marrow infiltration. Br J Haematol 84:75–82PubMedCrossRefGoogle Scholar
  40. 40.
    Van der Valk, P, Mullink, H, Huijgens, PC, Tadema, TM, Vos, W, Meijer, CJLM (1989) Immunohistochemistry in bone marrow diagnosis. Am J Surg Pathol 13:97–106PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • W. Eisterer
  • W. Hilbe
  • H. Huber
  • J. Thaler

There are no affiliations available

Personalised recommendations