Skip to main content

Somatic Embryogenesis from Leaf Protoplasts of Rauwolfia vomitoria Afz

  • Chapter
Somatic Embryogenesis and Synthetic Seed II

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 31))

Abstract

The genus Rauwolfia belongs to the family Apocynaceae and includes a very large number of tree and shrub species. Due to the presence of numerous alkaloids, Rauwolfia is used extensively for therapeutic purposes, some species are also used as ornamental plants in tropical and subtropical gardens or as forest species (Rao 1956).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Adjanohoun EJ (1986) Contribution aux études ethnobotaniques et floristiques en République Populaise du Congo, ACCT, Paris

    Google Scholar 

  • Agrawal S, Chandra N, Kothari SL (1989) Plant regeration in tissue cultures of pepper (Capsicum annuum L.cv. mathania). Plant Cell Tissue Organ Cult 16: 47–55

    Article  Google Scholar 

  • Arens H, Deus-Neumann D, Zenk MH (1987) Radio immunoassay for the quantitative determination of ajmaline. Planta Med 53 (2): 179–183

    Article  PubMed  CAS  Google Scholar 

  • Arya S, Liu JR, Eriksson T (1991) Plant regeneration from protoplasts of Panax ginseng (C.V. Meyer) through somatic embryogenesis. Plant Cell Rep 10: 277–281

    Article  Google Scholar 

  • Babcock PA, Carew DP (1962) Tissue culture of the Apocynaceae I. Culture requirements and alkaloid analysis. Lloydia 25: 209–213

    CAS  Google Scholar 

  • Bajaj YPS, Furmanowa M, Olszowska O (1988) Biotechnology of the micropropagation of medicinal and aromatic plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 60–103

    Google Scholar 

  • Boulay MP, Gupta PK, Krogstrup P, Durzan DJ (1988) Development of somatic embryos from cell suspension cultures of Norway spruce (Picea abies Karst). Plant Cell Rep 7: 134–137

    Article  Google Scholar 

  • Bruneton J (1987) Eléments de phytochimie et de pharmacognosie. Lavoisier, Paris

    Google Scholar 

  • Chen WH, Davey MR, Power JB, Cocking EC (1988) Control and maintenance of plant regeneration in sugarcane callus culture. J Exp Bot 199: 251–261

    Article  Google Scholar 

  • Conger BV, Hanning GE, Gray DJ, Maniel JK (1983) Direct embryogenesis from mesophyll cells of orchardgrass. Science 221: 850–851

    Article  PubMed  CAS  Google Scholar 

  • Court WE (1983) Alkaloid distribution in some African Rauwolfia species. Planta Med 48: 228–233

    Article  PubMed  CAS  Google Scholar 

  • Crèche J, Trémouillaux-Guiller J, Grôger D, Chénieux JC, Rideau M (1993) Choisya ternata (“Mexican Orange”): culture in vitro and the production of alkaloids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 21. Medicinal and aromatic plants V. Springer, Berlin Heidelberg New York, pp 107–121

    Google Scholar 

  • Diaz I, Moreno R, Power JB (1988) Plant regeneration from protoplasts of Capsicum annuum. Plant Cell Rep 7: 210–212

    Article  Google Scholar 

  • Dijak M, Brown CW (1987) Patterns of direct and indirect embryogenesis from mesophyll protoplasts of Medicage sativa. Plant Cell Tissue Organ CULT 9: 121–130

    Article  Google Scholar 

  • Finkelstein RR, Crouch ML (1986) Rapeseed embryo development in culture or high osmoticum is similar to that in seeds. Plant Physiol 81: 907–912

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Murashige T, Thorpe TA, Vasil IK (1976) Plant tissue culture media. In Vitro 12: 473–478

    Article  PubMed  CAS  Google Scholar 

  • Habib MS, Court WE (1973) Minor alkaloid ofRauwolfia caffra. Phytochemistry 12: 1821

    Article  CAS  Google Scholar 

  • Hampp N, Zenk MH (1988) Homogeneous strictosidine synthase from cell suspension cultures of Rauwolfia serpentina. Phytochemistry 27: 3811–3815

    Article  CAS  Google Scholar 

  • Heble MR (1985) Multiple shoot cultures: a viable alternative in vitro system for the production of known and new biologically active plant constituents. In: Neumann KH, Barz W, Reinhard E (eds) Primary and secondary metabolism of plant cell cultures. Springer, Berlin Heidelberg New York, pp 282–289

    Google Scholar 

  • Heble MR, Benjamin BD, Roja PC, Chadha MS (1983) Studies on shoot organ cultures of Atropa belladonna and Rauwolfia serpentina. In: Sen SK, Giles KL (eds) Plant cell culture in crop improvement. Plenum Press, New York, pp 57–63

    Google Scholar 

  • Iwu M (1980) Alkaloids of Rauwolfia vomitoria fruits. Planta Med Suppl: 13–16

    Google Scholar 

  • Iwu M, Court WE (1977) Root alkaloids of Rauwolfia vomitoria Afz. Planta Med 32: 88–89

    Article  PubMed  CAS  Google Scholar 

  • Iwu M, Court WE (1978a) The alkaloids of Rauwolfia cumminsii. Planta Med 34: 390–396

    Article  CAS  Google Scholar 

  • Iwu M, Court WE (1978b) Alkaloids of Rauwolfia cumminsii stem. Phytochemistry 17: 1651–1654

    Article  CAS  Google Scholar 

  • Iwu M, Court WE (1978c) Leaf alkaloids of Rauwolfia cumminsii stapf. Planta Med 33: 360–364

    Article  CAS  Google Scholar 

  • Iwu M, Court WE (1979) Alkaloids of Rauwolfia monbasiana stem bark. Planta Med 36: 208–212

    Article  CAS  Google Scholar 

  • Kaul K (1987) Plant regeneration from cotyledon hypocotyl expiants of Pinus strobus L. Plant Cell Rep 6: 5–7

    Article  Google Scholar 

  • Kobayashi S, Ikeda I, Uchimiya H (1985) Conditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts. Plant Cell Tissue Organ Cult 4: 249–259

    Article  Google Scholar 

  • Kunakh VA, Alkhimova EG (1989) Rauwolfia serpentina: in vitro culture and the production of ajmaline. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 7. Medicinal and aromatic plants II. Springer, Berlin Heidelberg New York, pp 398–416

    Google Scholar 

  • Lu DY, Thorpe TA (1987) Somatic embryogenesis and plantlet regeneration in cultured immature embryos ofPicea glauca. J Plant Physiol 128: 297–302

    CAS  Google Scholar 

  • Lu DY, Davey MR, Pental D, Cocking EC (1982) Forage legume protoplasts: somatic embryogenesis from protoplasts of seedling cotyledons and roots of Medicago sativa. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 597–598

    Google Scholar 

  • Majumdar SP, Poisson J, Potier P (1973) Alcaloïdes de Rauwolfia suaveolens. Phytochemistry 12: 1167–1169

    Article  CAS  Google Scholar 

  • Mitra GC, Kaul KN (1964) In vitro culture of root and stem callus of Rauwolfia serpentina Benth. for reserpine. Indian J Exp Biol 2: 49–51

    Google Scholar 

  • Miura Y, Tabata M (1986) Direct somatic embryogenesis from protoplasts of Foeniculum vulgare. Plant Cell Rep 5: 310–313

    Article  CAS  Google Scholar 

  • Mo LH, Von Arnold S (1991) Origin and development of embryogenic cultures from seedlings of norway spruce (Picea abies). J Plant Physiol 138: 223–230

    Google Scholar 

  • Moyne AL, Thor V, Pelissier B, Bergounioux C, Freyssinet G, Gadal P (1988) Callus and embryoids from protoplasts of Helianthus annuus. Plant Cell Rep 7: 437–440

    Google Scholar 

  • Müller JM, Schlittler E, Bein H J (1952) Reserpin, der sedative Wirkstoff au Rauwolfia serpentina Benth. Experientia 8/9: 338

    Article  PubMed  Google Scholar 

  • Murashige T, Tucker DPH (1969) Growth factor requirements of Citrus tissue cultures. In: Chapmann JV (ed) First Int Citrus Symp. Riverside, (A) pp 1155–1161

    Google Scholar 

  • Ochatt S J, Caso OH (1986) Shoot regeneration from leaf mesophyll protoplasts of wild pear (Pyrus communis var. pyraster L.). J Plant Physiol 122: 243–246

    CAS  Google Scholar 

  • Ohta S, Yatazawa M (1979) Growth and alkaloid production in callus tissues of Rauwolfia serpentina. Agric Biol Chem 11: 2297–2303

    Article  Google Scholar 

  • Paris RR, Moyse H (1971) Précis de Matière Médicale. Masson, Paris

    Google Scholar 

  • Pfitzner A, Stöckigt J (1983) Characterization of polyneuridine aldehyde esterase, a key enzyme in the biosynthesis of sarpagine/ajmaline type alkaloids. Planta Med 48: 221–227

    Article  PubMed  CAS  Google Scholar 

  • Poisson J (1959) Recherches sur les alcaloïdes des racines du Rauwolfia vomitoria Afz (Apocynacées). Thèse Doct Etat ès Sci Nat, Paris

    Google Scholar 

  • Rao AS (1956) A revision of Rauwolfia with particular reference to the American species. Ann Mo Bot Gard 43: 253–354

    Article  Google Scholar 

  • Rastushnyak YI, Piven NM, Rudas VA (1989) Protoplast culture and plant regeneration in Lycium barbarum L. Plant Cell Tissue Organ Culture 17: 183–190

    Article  Google Scholar 

  • Roja PC, Benjamin BD, Heble MR, Chadha MS (1985) Indole alkaloids from multiple shoot cultures of Rauwolfia serpentina. Planta Med 1: 73

    Article  Google Scholar 

  • Sabri NN, Court WE (1978) Stem alkaloids of Rauwolfia vomitoria. Phytochemistry 17: 2023–2026

    Article  CAS  Google Scholar 

  • Schiavone FM, Cooke TJ (1985) A geometric analysis of somatic embryo formation in carrot cell cultures. Can J Bot 63: 1573–1578

    Article  Google Scholar 

  • Schübel H, Ruyter CM, Stöckigt J (1989) Improved production of raucaffricine by cultivated Rauwolfia cells. Phytochemistry 28 (2): 491–494

    Article  Google Scholar 

  • Servier J (1957) Contribution à l’étude du Rauwolfia serpentina et aperçus sur quelques espèces voisines. Thèse Doct, Univ Lille

    Google Scholar 

  • Song J, Sorensen EL, Liang GH (1990) Direct embryogenesis from mesophyll protoplasts in alfalfa (Medicago sativa L). Plant Cell Rep 9: 21–25

    Article  Google Scholar 

  • Stöckigt J, Pfitzner A, Firl J (1981) Indole alkaloïds from cell suspension cultures of Rauwolfia serpentina Benth Plant Cell Rep 1: 36–39

    Article  Google Scholar 

  • Stolarz A, Macewicz J, Lörz H (1991) Direct somatic embryogenesis and plant regeneration from leaf explants of Nicotiana tabacum L. J. Plant Physiol 137: 347–357

    CAS  Google Scholar 

  • Takenaka Y (1970) Sur la Rauvanine, alcaloïde du Rauwolfia vomitoria Afz. (Apocynacées). Doct Etat ès Sci Pharm, Paris

    Google Scholar 

  • Timmins P, Court WE (1974a) Alkaloids of Rauwolfia macrophylla. Planta Med 13: 281–282

    CAS  Google Scholar 

  • Timmins P, Court WE (1974b) Root bark alkaloids of Rauwolfia obscur a. Phytochemistry 3: 1997

    Article  Google Scholar 

  • Timmins P, Court WE (1976) Further alkaloids from the roots of Rauwolfia obscur a. Planta Med 29: 283–288

    Article  PubMed  CAS  Google Scholar 

  • Trémouillaux-Guiller J (1988) Etude comparative des méthodologies de sélection de cultures cellulaires végétales à haute capacité d’accumulation. Application à des souches et lignées clonales biosynthétisant des alcaloïdes dihydrofuroquinoléïques. Thèse Doct, Univ Tours

    Google Scholar 

  • Trémouillaux-Guiller J, Chénieux JC (1991) Somatic embryogenesis from leaf protoplasts of Rauwolfia vomitoria shoot cultures. Plant Cell Rep 10: 102–105

    Article  Google Scholar 

  • Trémouillaux-Guiller J, Kodja H, Andreu F, Crèche J, Chénieux JC, Rideau M (1988) Variability in tissue cultures ofChoisya ternata III. Comparing alkaloid production in cell lines obtained by various strategies. Plant Cell Rep 7: 456–458

    Google Scholar 

  • Woodson RE Jr, Youngken HW, Schlittler E, Schneider JA (1957) Rauwolfia: botany, pharmacognosy, chemistry and pharmacology. Little, Brown and Co, Boston

    Google Scholar 

  • Yamamoto O, Yamada Y (1986) Production of reserpine and its optimization in cultured Rauwolfia serpentina Benth. cells. Plant Cell Rep 5: 50–53

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Trémouillaux-Guiller, J., Chénieux, J.C. (1995). Somatic Embryogenesis from Leaf Protoplasts of Rauwolfia vomitoria Afz. In: Bajaj, Y.P.S. (eds) Somatic Embryogenesis and Synthetic Seed II. Biotechnology in Agriculture and Forestry, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78643-3_29

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-78643-3_29

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78645-7

  • Online ISBN: 978-3-642-78643-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics