Somatic Embryogenesis and Artificial Seeds in Coptis chinensis F.

  • S. Ke
  • Y. Gui
  • R. M. Skirvin
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 31)


Coptis chinensis F. (family Ranunculaceae) is a perennial herbaceous plant, 10 to 13 cm in height. Its stem is rhizomatic and often branched. It is an endemic species distributed in Anhui, Guizhou, Jiangsu, Jiangxi, Hubei, Hunan, Shaanxi, Sichuan, Zhejiang Provinces of China. It is a very important medicinal plant in China. The rhizomes of the plant contain berberine, coptine, and coptisine. These compounds are reputed to result in cooling of the blood, reducing stress and fatigue, removing of toxic substances, and purging sthenic fever and emotions as well as promoting salivation (Wang 1964). Considerable work has been done on the micropropagation and production of medicinal compounds in Coptis species (Ikuta et al. 1975; Yamamoto et al. 1981; Ikuta and Itokawa 1989).


Somatic Embryo Somatic Embryogenesis Cotyledonary Stage Artificial Seed Perennial Herbaceous Plant 
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  1. Din P (1984) Studies on tissue culture of Coptis chinensis. Chin Trad Herb Drugs 15: 28–29Google Scholar
  2. Ikuta A, Itokawa H (1989) Coptis: in vitro regeneration of plants and the production of berberine. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol. 7. Medicinal and aromatic plants II. Springer. Berlin Heidberg New York, pp 110–128Google Scholar
  3. Ikuta A, Syono K, Furuya T (1975) Alkaloids in plants regenerated from Coptis callus cultures. Phytochemistry 14: 1209–1210CrossRefGoogle Scholar
  4. Ke S, He Z, Xu L, Hou S, Gui Y, Guo Z (1990) Studies on artificial seeds of Coptis chinensis. In: Guo Z, Gui Y (eds) Plant somatic embryogenesis and artificial seeds. Science Press, Beijing, pp 35–42Google Scholar
  5. Ke S, Wu L, Gui Y, Guo Z, He Z, Xu L (1992) Synchronization of somatic embryogenesis in Coptis chinensis. J Wuhan Bot Res 10: 1–6Google Scholar
  6. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  7. Nakagawa K, Miura Y, Fukin H, Tabata M (1982) Clonal propagation of medicinal plants through the induction of somatic embryogenesis from the cultured plant cell. In: Fujiwara A (ed) Plant tissue cultures 1982. Maruzen, Tokyo, pp 701–702Google Scholar
  8. Redenbaugh, K, Viss P, Slade D, Fujii TA (1987) Scale-up: artificial seeds. In: Green CE, Somers DA, Hackett WP, Bresboer DD (eds) Plant tissue and cell culture. Alan R Liss, New York, pp 473–493.Google Scholar
  9. Skirvin, RM, Norton M, Mheeters KD (1993) Somaclonal variation: has it proved useful for plant improvement? Acta Hortic 336: 333–340Google Scholar
  10. Staba EJ (1977) Tissue culture and pharmacy. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 699–715Google Scholar
  11. Syono K, Furuya T (1972) The differentiation of Coptis plants in vitro from callus cultures. Experientia 28: 286CrossRefGoogle Scholar
  12. Tandon P, Rathore TS (1992) Regeneration of plantlets from hypocotyl-derived callus of Coptis teeta. Plant Cell Tissue organ Cult 28: 115–117CrossRefGoogle Scholar
  13. Wang G (1964) Studies on alkaloids of stem and root in Coptis chinensis. Acta Pharm Sin 11: 389–392Google Scholar
  14. Yamamoto H, Ishida M, Tomimori T (1981) Studies of the fundamental conditions on growth and alkaloid formation of the callus cultures of Coptis japónica var. Japónica (III). Effects of cloning and mutagens. Shoyakugaku Zassi 35: 15–21Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • S. Ke
    • 1
  • Y. Gui
    • 2
  • R. M. Skirvin
    • 3
  1. 1.Wuhan Institute of BotanyAcademia SinicaPeople’s Republic of China
  2. 2.Institute of BotanyAcademia SinicaBejingPeople’s Republic of China
  3. 3.Plant and Animal Biotechnology LaboratoryUniversity of IllinoisUrbanaUSA

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