Molecular and Genetic Determinants of the Listeria monocytogenes Infectious Process

  • B. Sheehan
  • C. Kocks
  • S. Dramsi
  • E. Gouin
  • A. D. Klarsfeld
  • J. Mengaud
  • P. Cossart
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 192)


Listeria monocytogenes was first characterized in 1926 following an outbreak of listeriosis in laboratory animals (MURRAY et al. 1926). However, it was not until the 1980s that an unambiguous link was established between the human disease and the consumption of Listeria-contaminated foodstuffs (SCHLECH et al. 1983). Immunosuppressed individuals, pregnant women, foetuses and neonates are most susceptible to Listeria infection. Human listeriosis is characterized by a high mortality rate, with clinical features including meningitis or meningo-encephalitis, septicemia, abortion, and perinatal infections (GRAY and KILLINGER 1966). If diagnosed early, listeriosis can be successfully treated by the administration of high doses of antibiotics, most frequently ampicillin or penicillin, either alone or in combination with aminoglycosides.


Listeria Monocytogenes Hemolytic Activity Shigella Flexneri Actin Assembly Spotted Fever Group 
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  1. Aiba H, Fujimoto S, Ozaki N (1982) Molecular cloning and sequencing of the gene for E. coli cAMP receptor protein. Nucl Acids Res 10: 1345–1361Google Scholar
  2. Allaoui A, Mounier J, Prevost MC, Sansonetti PJ, Parsot C (1992) icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intracellular spread. Mol Microbiol 6: 1605–1617PubMedGoogle Scholar
  3. Beaman LV, Beaman BL (1990) Monoclonal antibodies demonstrate that superoxide dismutase contributes to protection of Nocardia asteroides within the intact host. Infect Immun 58: 3122–3128PubMedGoogle Scholar
  4. Bernardini ML, Mounier J, D’Hauteville H, Coquis-Rondon M, Sansonetti PJ (1989) Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. Proc Natl Acad Sci USA 86: 3867–3871PubMedGoogle Scholar
  5. Bielecki J, Youngman P, Connelly P, Portnoy DA (1990) Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells. Nature 345: 175–176PubMedGoogle Scholar
  6. Bliska JB, Galan JE, Falkow S (1993) Signal transduction in the mammalian cell during bacterial attachment and entry. Cell 73: 903–920PubMedGoogle Scholar
  7. Bohne J, Sokolivic Z, Goebel W (1994) Transcriptional regulation of prfA and prfA-regulated virulence genes in Listeria monocytogenes. Mol Microbiol 11: 1141–1150PubMedGoogle Scholar
  8. Brehm K, Haas A, Goebel W, Kreft J (1992) A gene encoding a superoxide dismutase of the facultative intracellular bacterium Listeria monocytogenes. Gene 118: 121–125PubMedGoogle Scholar
  9. Brundage RA, Smith GA, Camilli A, Theriot JA, Portnoy DA (1993) Expression and phosphorylation of the Listeria monocytogenes ActA protein in mammalian cells. Proc Natl Acad Sci USA 90: 11890–11894PubMedGoogle Scholar
  10. Bubert A (1992) Structural and functional properties of the p60 proteins from different listeria species. J Bacteriol 174: 8166–8171PubMedGoogle Scholar
  11. Camilli A, Tilney L, Portnoy D (1993) Dual roles of pIcA in Listeria monocytogenes pathogenesis. Mol Microbiol 8: 143–157PubMedGoogle Scholar
  12. Chakraborty T, Goebel W (1988) Recent developments in the study of virulence in Listeria monocytogenes. In: Goebel W (ed) Intracellular bacteria. Springer Berlin Heidelberg New York, pp 41–58 (Currenttopics in microbiologyand immunology, vol 138 )Google Scholar
  13. Chakraborty T, Leimeister-Wachter M, Domann E, Hartl M, Goebel W, Nichterlein T, Notermans S (1992) Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prf A gene. J Bacteriol 174: 568–574PubMedGoogle Scholar
  14. Collins MD, Wallbanks S, Lane DJ, Shah J, Nietupski R, Smida J, Dorsch M, Stackebrandt E (1991) Phylogenetic analysis of the genus Listeria based on reverse transcriptase sequencing of 16S rRNA. Int J Syst Bacteriol 41: 240–246PubMedGoogle Scholar
  15. Conlan JW, North RJ (1991) Neutrophil-mediated dissolution of infected host cells as a defense strategy against a facultative intracellular bacterium. J Exp Med 174: 741–744PubMedGoogle Scholar
  16. Conlan W, North R (1993) Neutrophil-mediated lysis of infected hepatocytes. ASM News 59: 563-567 Cooper RF, Dennis SM (1978) Further characterization of Listeria monocytogenes serotype 5. Can J Microbiol 24: 598–599Google Scholar
  17. Cossart P (1994) Listeria monocytogenes: strategies for entry and survival in cells and tissues. In: Russell D (ed) Baillere’s Clinical infectious Diseases, Strategies for intracellular survival of microbes. Bailliere Tindall Ltd, London (in press)Google Scholar
  18. Cossart P, Gicquel-Sanzey B (1982) Cloning and sequence of the crp gene of Escherichia coli K 12. Nucl Acids Res 10: 1363–1378PubMedGoogle Scholar
  19. Cossart P, Mengaud J (1989) Listeria monocytogenes: a model system for the molecular study of intracellular parasitism. Mol Biol Med 6: 463–474PubMedGoogle Scholar
  20. Cossart P, Kocks C (1994) The actin based motility of the intracellular pathogen Listeria monocytogenes. Mol Microbiol (in press)Google Scholar
  21. Cossart P, Vicente MF, Mengaud J, Baquero F, Perez-Diaz JC, Berche P (1989) Listeriolysin O is essential for the virulence of Listeria monocytogenes: direct evidence obtained by gene complementation. Infect Immun 57: 3629–3636PubMedGoogle Scholar
  22. d’Hauteville H, Sansonetti PJ (1992) Phosphorylation of IcsA by cAMP-dependent protein kinase and its effecton intracellular spread of Shigella flexneri. Mol Microbiol 6: 833–841PubMedGoogle Scholar
  23. Dabiri GA, Sanger JM, Portnoy DA, Southwick FS (1990) Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly. Proc Natl Acad Sci USA 87: 6068–6072PubMedGoogle Scholar
  24. Domann E, Leimeister-Wachter M, Goebel W, Chakraborty T (1991) Molecular cloning, sequencing, and identification of a metalloprotease gene from Listeria monocytogenes that is species specific and physically linked to the listeriolysin gene. Infect Immun 59: 65–72PubMedGoogle Scholar
  25. Domann E, Wehland J, Rohde M, Pistor S, Hartl M, Goebel W, Leimeister-Wächter M, Wuenscher M, Chakraborty T (1992) A novel bacterial gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin. EMBO J 11: 1981–1990PubMedGoogle Scholar
  26. Dons L, Rasmussen OF, Olsen JE (1992) Cloning and characterization of a gene encoding flagellin of Listeria monocytogenes. Mol Microbiol 6: 2919–2929PubMedGoogle Scholar
  27. Dramsi S, Dehoux P, Cossart P (1993a) Common features of Gram-positive bacterial proteins involved in cell recognition. Mol Microbiol 9: 1119–1122PubMedGoogle Scholar
  28. Dramsi S, Kocks C, Forestier C, Cossart P (1993b) Internalin-mediated invasion of epithelial cells by Listeria monocytogenes is regulated by the bacterial growth state, temperature and the pleiotropic activator, prfA. Mol Microbiol 9: 931–941PubMedGoogle Scholar
  29. Drevets D, Campbell PA (1991) Roles of complement and complement receptor type 3 in phagocytosis of Listeria monocytogenes by inflammatory mouse macrophages. Infect Immun 59: 2645–2652PubMedGoogle Scholar
  30. Drevets D, Canono BP, Campbell PA (1992) Listericidal and nonlistericidal mouse macrophages differ in complement receptor type3-mediated phagocytosis of L. mono-cytogenes and in preventing escape of the bacteria into the cytoplasm. J Leukoc Biol 52: 70–79PubMedGoogle Scholar
  31. Eaton DA, Morgan DR, Krakowka S (1992) Motility as a factor in the colonisation of gnotobiotic piglets by Helicobacter pylori. J Med Microbiol 37: 123–127PubMedGoogle Scholar
  32. Falkow S, Isberg RR, Portnoy DA (1992) The interaction of bacteria with mammalian cells. Annu Rev Cell Biol 8: 333–363PubMedGoogle Scholar
  33. Fischetti VA, Pancholi V, Schneewind O (1990) Conservation of a hexapeptide sequence in the anchor region of surface proteins from gram-positive cocci. Mol Microbiol 4: 1603–1605PubMedGoogle Scholar
  34. Formal SB, Gemski J, Baron LS, LaBrec EH (1971) A chromosomal locus which controls the ability of Shigella flexneri to evoke keratoconjuctivitis. Infect Immun 3: 73–79PubMedGoogle Scholar
  35. Franzon VL, Arondel J, Sansonetti PJ (1990) Contribution of superoxide dismutase and catalase to Shigella flexneri pathogenesis. Infect Immun 58: 529–535PubMedGoogle Scholar
  36. Freitag NE, Portnoy DA (1994) Dual promoters of the Listeria monocytogenes prfA transcriptional activator appear essential in vitro but are redundant in vivo. Mol Microbiol 12: 845–853PubMedGoogle Scholar
  37. Freitag NE, Youngman P, Portnoy DA (1992) Transcriptional activation of the Listeria monocytogenes hemolysin gene in Bacillus subtilis. J Bacteriol 174: 1293–1298PubMedGoogle Scholar
  38. Freitag NE, Rong L, Portnoy DA (1993) Regulation of the prfA transcriptional activator of Listeria monocytogenes: multiple promoter elements contribute to intracellular growth and cell-to-cell spread. Infect Immun 61: 2537–2544PubMedGoogle Scholar
  39. Fuzi M, Pillis I (1962) Production of opacity in egg-yolk medium by Listeria monocytogenes. Nature 13: 195Google Scholar
  40. Gaillard JL, Berche P, Sansonetti P (1986) Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect Immun 52: 50–55PubMedGoogle Scholar
  41. Gaillard JL, Berche P, Mounier J, Richard S, Sansonetti PJ (1987) In vitro model of penetration and intracellular growth of L. monocytogenes in the human enterocyte-like cell line Caco-2. Infect Immun 55: 2822–2829PubMedGoogle Scholar
  42. Gaillard J-L, Berche P, Frehel C, Gouin E, Cossart P (1991) Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci. Cell 65: 1127–1141PubMedGoogle Scholar
  43. Garcia del Portillo F, Sanchez-Campillo M, Baquero F, Perez-Diaz JC (1992) New genes of Listeria monocytogenes presumptively related with enteric pathogenicity. In: Witholt B, Alouf JE, Boulnois GJ, Cossart P, Dijkstra BW, Falmagne P, Fehrenbach FJ, Freer J, Niemann H, Rappuoli R, Wadstrom T (eds) Bacterial protein toxins, 5th European workshop. Fischer, Stuttgart, pp 500–505Google Scholar
  44. Geoffroy C, Gaillard JL, Alouf JE, Berche P (1987) Purification, characterization and toxicity of the sulfhydryl-activated hemolysin listeriolysin 0 from Listeria monocytogenes. Infect Immun 55: 1641–1646PubMedGoogle Scholar
  45. Geoffroy C, Gaillard JL, Alouf J, Berche P (1989) Production of thiol-dependent hemolysins by Listeria monocytogenes and related species. J Gen Microbiol 135: 481–487PubMedGoogle Scholar
  46. Geoffroy C, Raveneau J, Beretti JL, Lecroisey A, Vazquez-Boland JA, Alouf JE, Berche P (1991) Purification and characterization of an extracellular 29-Kilodalton phospholipase C from L. monocytogenes. Infect Immun 59: 2382–2388PubMedGoogle Scholar
  47. Goebel W, Kreft J, Bohne J, Demuth A, Kestler H, Sokolovic Z (1994) Regulation of cytolysins and other virulence factors in Listeria monocytogenes. In: Freer F et al. (eds) Bacterial protein toxins. Fischer, Stuttgart, pp 138–145 (ZbL Bakt Suppl 24 )Google Scholar
  48. Goldberg MB, Barzu O, Parsot C, Sansonetti PJ (1993) Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement. J Bacteriol 175: 2189–2196PubMedGoogle Scholar
  49. Goldfine H, Knob C (1992) Purification and characterization of Listeria monocytogenes phosphatidylinositol-specific phospholipase C. Infect Immun 60: 4059–4067PubMedGoogle Scholar
  50. Goldfine H, Johnston NC, Knob C (1993) The non-specific phospholipase C of Listeria monocytogenes: activity on phospholipids in triton X-100 mixed micelles and in biological membranes. J Bacteriol 175: 4298–4306PubMedGoogle Scholar
  51. Goossens PL, Milon G (1992) Induction of protective CD8+ T lymphocytes by an attenuated Listeria monocytogenes actA mutant. Int Immunol 4: 1413–1418PubMedGoogle Scholar
  52. Gormley E, Mengaud J, Cossart P (1989) Sequences homologous to the Listeriolysin O gene region of Listeria monocytogenes are present in virulent and avirulent haemolytic species of the genus Listeria. Res Microbiol 140: 631–643PubMedGoogle Scholar
  53. Gouin E, Mengaud J, Cossart P (1994) The virulence gene cluster of Listeria monocytogenes is also present in Listeria ivanovii, an animal pathogen, and Listeria seeligen, a non-pathogenic species. Infect immun (in press)Google Scholar
  54. Gray ML, Killinger AH (1966) Listeria monocytogenes and listeric infections. Bacteriol Rev 30: 309–382PubMedGoogle Scholar
  55. Haas A, Brehm K, Kreft J, Goebel W (1991) Cloning, characterization, and expression in Escherichia coli of a gene encoding Listeria seeligeri catalase, a bacterial enzyme highly homologous to mammalian catalases. J Bacteriol 173: 5159–5167PubMedGoogle Scholar
  56. Haas A, Dumbsky M, Kreft J (1992) Listeriolysin genes: complete sequence of ilo from Listeria ivanovii and Listeria seeligeri. Biochim Biophys Acta 1130: 81–84PubMedGoogle Scholar
  57. Hahn H, Kaufmann SHE (1981) The role of cell mediated immunity in bacterial infections. Rev Infect Dis 3: 1221–1250PubMedGoogle Scholar
  58. Havell EA (1986) Synthesis and secretion of interferon by murine fibroblasts in response to intracellular Listeria monocytogenes. Infect Immun 54: 787–792PubMedGoogle Scholar
  59. Heinzen RA, Hayes SF, Peacock MG, Hackstadt T (1993) Directional actin polymerization associated with spotted fever group Rickettsia infection of vero cells. Infect Immun 61: 1926–1935PubMedGoogle Scholar
  60. Higgins CF (1992) ABC transporters: from microorganisms to man. Annu Rev Cell Biol 8: 67–113PubMedGoogle Scholar
  61. High N, Mounier J, Prévost MC, Sansonetti PJ (1992) IpaB of Shigella flexneri causes entry into epithelial cells and escape from the phagocytic vacuole. EMBO J 11: 1991–1999PubMedGoogle Scholar
  62. Irvine AS, Guest JR (1993) Lactobacillus casei contains a member of the CRP-FNR family. Nucl Acids Res 21: 753PubMedGoogle Scholar
  63. Jones D (1992) Current classification of the genus Listeria Conference proceedings, Listeria 1992. The 11th international symposium on problems of Listeriosis, Copenhagen, p 2Google Scholar
  64. Karunasagar I, Krohne G, Goebel W (1993) Listeria ivanovii is capable of cell-to-cell spread involving actin polymerization. Infect Immun 61: 162–169PubMedGoogle Scholar
  65. Kathariou S, Rocourt J, Hof H, Geobel W (1988) Levels of Listeria monocytogenes hemolysin are not directly proportional to virulence in experimental infections of mice. Infect Immun 56: 534–536PubMedGoogle Scholar
  66. Kathariou S, Pine VG, Carlone GM, Holloway BP (1990) Nonhemolytic Listeria mono-cytogenes mutants that are also noninvasive for mammalian cells in culture: evidence for coordinate regulation of virulence. Infect Immun 58: 3988–3995PubMedGoogle Scholar
  67. Kaufmann SHE (1993) Immunity to intracellular bacteria. Annu Rev Immunol 11: 129–163PubMedGoogle Scholar
  68. Klarsfeld AD, Goossens PL, Cossart P (1994) Five Listeria monocytogenes genes preferentially expressed in infected mammalian cells: pIcA, purH, purD, purE, and an arginine ABC transporter gene, arpJ. Mol Microbiol 13 (in press)Google Scholar
  69. Klier A, Msadek T, Rapoport G (1992) Positive regulation in the Gram-positive bacterium: Bacillus subtilis. Ann Rev Microbiol 46: 429–459Google Scholar
  70. Kocks C, Gouin E, Tabouret M, Berche P, Ohayon H, Cossart P (1992) Listeria mono-cytogenes-induced actin assembly requires the actA gene product, a surface protein. Cell 68: 521–531PubMedGoogle Scholar
  71. Kocks C, Hellio R, Gounon P, Ohayon H, Cossart P (1993) Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly. J Cell Sei 105: 699–710Google Scholar
  72. Köhler S, Leimeister-Wächter M, Chakraborty T, Lottspeich F, Goebel W (1990) The gene coding for protein p60 of Listeria monocytogenes and its use an a specific probe for Listeria monocytogenes. Infect Immun 58: 1943–1950PubMedGoogle Scholar
  73. Köhler S, Bubert A, Vogel M, Goebel W (1991) Expression of the iap gene coding for protein p60 of Listeria monocytogenes is controlled on the posttranscriptional level. J Bacteriol 173: 4668–4674PubMedGoogle Scholar
  74. Kuhn M, Goebel W (1989) Identification of an extracellular protein of Listeria monocytogenes possibly involved in intracellular uptake by mammalian cells. Infect Immun 57: 55–61PubMedGoogle Scholar
  75. Kuhn M, Kathariou S, Goebel W (1988) Hemolysin supports survival but not entry of the intracellular bacterium Listeria monocytogenes. Infect Immun 56: 79–82PubMedGoogle Scholar
  76. Kuhn M, Prévost M-C, Mounier J, Sansonetti PJ (1990) A nonvirulent mutant of Listeria monocytogenes does not move intracellularly but still induces polymerization of actin. Infect Immun 58: 3477–3486PubMedGoogle Scholar
  77. Lampidid R, Gross R, Sokolovic Z, Goebel W, Kreft J (1994) The virulence regulator protein of Listeria ivanovii is highly homologous to PrfA from Listeria monocytogenes and both belong to the Crp-Fnr family of transcription regulators. Mol Microbiol 13: 141–151Google Scholar
  78. Leblond-Francillard M, Gaillard J-L, Berche P (1989) Loss of catalase activity in Tn1545-induced mutants does not reduce growth of Listeria monocytogenes in vivo. Infect Immun 57: 2569–2573PubMedGoogle Scholar
  79. Leimeister-Wachter M, Chakraborty T (1989) Detection of listeriolysin, the thiol-dependent hemolysin in Listeria monocytogenes, Listeria ivanovii and Listeria seeligeri. Infect Immun 57: 2350–2357PubMedGoogle Scholar
  80. Leimeister-Wachter M, Goebel W, Chakraborty T (1989) Mutations affecting hemolysin production in Listeria monocytogenes located outside the listeriolysin gene. FEMS Microbiol Lett 65: 23–30Google Scholar
  81. Leimeister-Wachter M, Haffner C, Domann E, Goebel W, Chakraborty T (1990) Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of Listeria monocytogenes. Proc Natl Acad Sci USA 87: 8336–8340PubMedGoogle Scholar
  82. Leimeister-Wachter M, Domann E, Chakraborty T (1991) Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is coordinately expressed with listeriolysin in Listeria monocytogenes. Mol Microbiol 5: 361–366PubMedGoogle Scholar
  83. Leimeister-Wachter M, Domann E, Chakraborty T (1992) The expression of virulence genes in L. monocytogenes is thermoregulated. J Bacteriol 174: 947–952PubMedGoogle Scholar
  84. Lett M-C, Sasakawa C, Okada N, Sakai T, Makino S, Yamada M, Komatsu K, Yoshikawa M (1989) virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence. J Bacteriol 171: 353–359Google Scholar
  85. Lindberg AA, Karnell A, Stocker BAD, Katakura S, Sweiha H, Reinholt FP (1988) Development of an auxotrophic oral live Shigella flexneri vaccine. Vaccine 6: 146–150PubMedGoogle Scholar
  86. Liu S-L, Ezaki T, Miura H, Matsui K and Yabuuchi E (1988) Intact motility as a Salmonella typhi virulencerelated factor. Infect Immun 56: 1967–1973PubMedGoogle Scholar
  87. MacDonald TT, Carter PB (1980) Cell-mediated immunity to intestinal infection. Infect Immun 28: 516–523PubMedGoogle Scholar
  88. Mackaness GB (1962) Cellular resistance to infection. J Exp Med 116: 381–406PubMedGoogle Scholar
  89. Marco AJ, Prats N, Ramos JA, Briones V, Blanco M, Dominguez L, Domingo M (1992) A microbiological, histopathological and immunohistological study of the intragastric inoculation of L. monocytogenes in mice. J Comp Pathol 107: 1–9PubMedGoogle Scholar
  90. Marquis H, Bouwer HA, Hinrichs D, Portnoy D (1993) Intracytoplasmic growth and virulence of Listeria monocytogenes auxotrophic mutants. Infect Immun 61: 3756–3760PubMedGoogle Scholar
  91. Mekalanos JJ (1992) Environmental signals controlling expression of virulence determinants in bacteria. J Bacteriol 174: 1–7PubMedGoogle Scholar
  92. Mengaud J, Vicente MF, Cossart P (1989) Transcriptional mapping and nucleotide sequences of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation. Infect Immun 57: 3695–3701PubMedGoogle Scholar
  93. Mengaud J, Braun-Breton C, Cossart P (1991a) Identification of a phosphatidylinositol-specific phospholipase C in Listeria monocytogenes: a novel type of virulence factor? Mol Microbiol 5: 367–372PubMedGoogle Scholar
  94. Mengaud J, Dramsi S, Gouin E, Vazquez-Boland JA, Milon G, Cossart P (1991b) Pleiotropic control of Listeria monocytogenes virulence factors by a gene which is autoregulated. Mol Microbiol 5: 2273–2283PubMedGoogle Scholar
  95. Mengaud J, Geoffroy C, Cossart P (1991c) Identification of a novel operon involved in virulence of Listeria monocytogenes: its first gene encodes a protein homologous to bacterial metalloproteases. Infect Immun 59: 1043–1049PubMedGoogle Scholar
  96. Michel E, Cossart P (1992) Physical map of the Listeria monocytogenes chromosome. J Bacteriol 174: 7098–7103PubMedGoogle Scholar
  97. Michel E, Reich KA, Favier R, Berche P, Cossart P (1990) Attenuated mutants of the intracellular bacterium Listeria monocytogenes obtained by single amino-acid substitutions in listeriolysin O. Mol Microbiol 4: 2167–2178PubMedGoogle Scholar
  98. Mounier J, Ryter A, Coquis-Rondon M, Sansonetti PJ (1990) Intracellular and cell-to-cell spread of Listeria monocytogenes involves interaction with F-actin in the enterocyte-like cell line Caco-2. Infect Immun 58: 1048–1058PubMedGoogle Scholar
  99. Murray EGD, Webb RE, Swann MBR (1926) A disease of rabbits characterized by a large mononuclear leucocytosis, caused by a hitherto undescribed bacillus Bacterium monocytogenes (n. sp.). J Pathol Bacteriol 29: 407–439Google Scholar
  100. Niebuhr K, Chakraborty T, Kollner P, Wehland J (1993) Production of Monoclonal antibodies to the phosphatidyl choline-specific phospholipase C of Listeria monocytogenes, a virulence factor for this species. Med Microbiol Lett 2: 9–16Google Scholar
  101. Park SF, Kroll RG (1993) Expression of listeriolysin and phosphatidylinositol-specific phospholipase C is repressed by the plant-derived molecule cellobiose in Listeria monocytogenes. Mol Microbiol 8: 653–661PubMedGoogle Scholar
  102. Parkinson JS (1993) Signal transduction schemes of bacteria. Cell 73: 857–871PubMedGoogle Scholar
  103. Pistor S, Chakraborty T, Niebuhr K, Domann E, Wehland J (1994) The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton. EMBO J 13: 758–763PubMedGoogle Scholar
  104. Portnoy DA (1992) Innate immunity to a facultative intracellular bacterial pathogen. Curr Opin Immunol 4: 20–24PubMedGoogle Scholar
  105. Portnoy D, Jacks PS, Hinrichs D (1988) Role of hemolysin for the intracellular growth of L. monocytogenes. J Exp Med 167: 1459–1471PubMedGoogle Scholar
  106. Portnoy DA, Chakraborty T, Goebel W, Cossart P (1992a) Molecular determinants of Listeria monocytogenes pathogenesis. Infect Immun 60: 1263–1267PubMedGoogle Scholar
  107. Portnoy DA, Tweten R, Kehoe M, Bielecki J (1992b) Capacity of listeriolysin, streptolysin O and perfringolysin 0 to mediate growth of Bacillus subtilis within mammalian cells. Infect Immun 60: 2710–2717PubMedGoogle Scholar
  108. Poyart C, Abachin E, Razafimanantsoa I, Berche P (1993) The Zinc Metalloprotease of Listeria monocytogenes is required for maturation of Phosphatidylcholine phospholipase C: direct evidence obtained by gene complementation. Infect Immun 61: 1576–1580PubMedGoogle Scholar
  109. Racz P, Tenner K, Szivessy K (1970) Electron microscopic studies in experimental keratoconjunctivitis listeriosa. I. Penetration of Listeria monocytogenes into corneal epithelial cells. Acta Microbiol Acad Sci Hung 17: 221–236PubMedGoogle Scholar
  110. Racz P, Tenner K, Mero E (1972) Experimental Listeria enteritis. I. An electron microscopic study of the epithelial phase in experimental Listeria infection. Lab Invest 26: 694–700PubMedGoogle Scholar
  111. Racz P, Kaiserling E, Tenner K, Wuthe HH (1973) Experimental Listeria cystitis. II. Further evidence of the epithelial phase in experimental Listeria infection. An electron microscopic study. Virchows Arch [B] 13: 24–37Google Scholar
  112. Raveneau J, Geoffroy C, Beretti JL, Gaillard JL, Alouf JE, Berche P (1992) Reduced virulence of a Listeria monocytogenes phospholipase-deficient mutant obtained by transposon insertion into the zinc metalloprotease gene. Infect lmmun 60: 916–921Google Scholar
  113. Richardson K (1991) Roles of motility and flagellar structure in pathogenicity of Vibrio cholerae: Analysis of motility mutants in three animal models. Infect Immun 59: 2727–2736PubMedGoogle Scholar
  114. Rosen H, Gordon S, North RJ (1989) Exacerbation of murine listeriosis by a monoclonal antibody specific for the type 3 complement receptor of myelomonocytic cells. Absence of monocytes at infective foci allows Listeria to multiply in nonphagocytic cells. J Exp Med 170: 27–37PubMedGoogle Scholar
  115. Ruhland GJ, Hellwig M, Wanner G, Fiedler F (1993) Cell-surface location of Listeria-specific protein p60- detection of Listeria cells by indirect immunofluorescence. J Gen Microbiol 139: 609–616PubMedGoogle Scholar
  116. Sanger JM, Sanger JW, Southwick FS (1992) Host cell actin assembly is necessary and likely to provide the propulsive force for intracellular movement of Listeria monocytogenes. Infect Immun 60: 3609–3619PubMedGoogle Scholar
  117. Sansonetti PJ (1992) Molecular and cellular biology of Shigella flexneri invasivness In: Sansonetti PJ (ed) Pathogenesis of Shigellosis. Springer, Berlin Heidelberg New York, pp 1–20 (Current topics in microbiology and immunology, vol 180)Google Scholar
  118. Sansonetti PJ, Ryter A, Clerc P, Maurelli AT, Mounier J (1986) Multiplication of Shigella flexneri within HeLa cells: lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis. Infect Immun 51: 461–469PubMedGoogle Scholar
  119. Schlech WF III, Lavigne PM, Bortolussi RA, Allen AC, Haldane VE, Wort JA, Hightower AW, Johnson SE, King SH, Nicholls ES, Broome C (1983) Epidemic listeriosis-evidence for transmission by food. N Engl J Med 308: 203–206PubMedGoogle Scholar
  120. Silverman DJ, Santucci LA, Meyers N, Sekeyova Z (1992) Penetration of host cells by Rickettsia rickettsii appears to be mediated by a phospholipase of Rickettsial origin. Infect Immun 60: 2733–2740PubMedGoogle Scholar
  121. Smyth CJ, Duncan JL (1978) Thiol-activated (oxygen labile) cytolysins. In: Jeljaszewicz J, Wadstrom T (eds) Bacterial toxins and cell membranes. Academic, New YorkGoogle Scholar
  122. Sokolovic Z, Goebel W (1989) Synthesis of listeriolysin in Listeria monocytogenes under heat shock conditions. Infect Immun 57: 295–298PubMedGoogle Scholar
  123. Sokolovic Z, Fuchs A, Goebel W (1990) Synthesis of species-specific stress proteins by virulent strains of Listeria monocytogenes. Infect Immun 58: 3582–3587PubMedGoogle Scholar
  124. Sokolovic Z, Riedel J, Wuenscher M, Goebel W (1993) Surface-associated, PrfA-regulated proteins of Listeria monocytogenes synthesized under stress conditions. Mol Microbiol 8: 219–227PubMedGoogle Scholar
  125. Southwick FS, Purich DL (1994) Arrest of Listeria movement in host cells by a bacterial ActA analogue: Implications for actin-based motility. Proc Natl Acad Sci USA 91: 5168–5172PubMedGoogle Scholar
  126. Stock JB, Ninfa AJ, Stock AM (1989) Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev 53: 450–490PubMedGoogle Scholar
  127. Stock JB, Stock AM, Mottonen J (1990) Signal transduction in bacteria. Nature 344: 395–400PubMedGoogle Scholar
  128. Sun AN, Camilli A, Portnoy DA (1990) Isolation of Listeria monocytogenes small-plaque mutants defective in intracellular growth and cell-to-cell spread. Infect Immun 58: 3770–3778PubMedGoogle Scholar
  129. Teysseire N, Chiche-Portiche C, Raoult D (1992) Intracellular movements of Rickettsia conorii and R. typhi based on actin polymerization. Res Microbiol 143: 821–829PubMedGoogle Scholar
  130. Theriot J, Mitchison TJ (1992) The nucleation-release model of actin filament dynamics in cell motility. Trends Cell Biol 2: 219–222PubMedGoogle Scholar
  131. Theriot JA, Mitchison TJ, Tilney LG, Portnoy DA (1992) The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization. Nature 357: 257–260PubMedGoogle Scholar
  132. Theriot JA, Rosenblatt J, Portnoy DA, Goldschmidt-Clermont PJ, Mitchison TJ (1994) Involvement of profilin in the actin-based motility of Listeria monocytogenes in cells and cell-free extracts. Cell 76: 505–517PubMedGoogle Scholar
  133. Tilney LG, Portnoy DA (1989) Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes. J Cell Biol 109: 1597–1608PubMedGoogle Scholar
  134. Tilney LG, Tilney MS (1993) The wily ways of a parasite: induction of actin assembly by Listeria. Trends Microbiol 1: 25–31PubMedGoogle Scholar
  135. Vancompernolle K, Goethals M, Huet C, Louvard D, Vandekerckhove J (1992) G-to F-actin modulation by a single amino acid substitution in the actin binding site of actobindin and thymosin p4. EMBO J 11: 4739–4746PubMedGoogle Scholar
  136. Vazquez-Boland J-A, Kocks C, Dramsi S, Ohayon H, Geoffroy C, Mengaud J, Cossart P (1992) Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread. Infect Immun 60: 219–230PubMedGoogle Scholar
  137. Vega-Palas MA, Flores E, Herrero A (1992) NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus that belongs to the Crp family of bacterial regulators. Mol Microbiol 6: 1853–1859PubMedGoogle Scholar
  138. Vicente MF, Baquero F, Perez-Diaz JC (1989) Molecular cloning of the Listeria monocytogenes DNA fragment presenting strong hybridization with V. cholerae toxin genes. In: Rappuoli R, Alouf JE, Falmagne P, Fehrenbach FJ, Freer J, Gross R, Jeljaszewicz J, Montecucco C, Tomasi M, Wadstrom T, Witholt B (eds) Bacterial protein toxins, 4th European workshop. Fischer, Stuttgart, pp 353–355Google Scholar
  139. Westerlund B, Korhonen TK (1993) Bacterial proteins binding to the mammalian extracellular matrix. Mol Microbiol 9: 687–694PubMedGoogle Scholar
  140. Winkler HH (1990) Rickettsia Species (as organisms). Annu Rev Microbiol 44: 131–153PubMedGoogle Scholar
  141. Wren BW, Colby SM, Cubberley RR, Pallen MJ (1992) Degenerate PCR primers for the amplification of fragments from genes encoding response regulators from a range of pathogenic bacteria. FEMS Microbiol Lett 99: 287–292Google Scholar
  142. Wuenscher M, Kohler S, Bubert A, Gerike U, Goebel W (1993) The iap gene of Listeria monocytogenes is essential for cell viability and its gene product, p60, has bacteriolytic activity. J Bacteriol 175: 3491–3501PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • B. Sheehan
    • 1
  • C. Kocks
    • 1
  • S. Dramsi
    • 1
  • E. Gouin
    • 1
  • A. D. Klarsfeld
    • 1
  • J. Mengaud
    • 1
  • P. Cossart
    • 1
  1. 1.Unité des Interactions Bacteria-CellulesCNRS URA 1300, Institut PasteurParis Cedex 15France

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