Abstract
In the search for protective antigens aiming at the development of a sub-unit vaccine against toxoplasmosis, several molecules have been found to represent good candidates, in particular P30 or SAG1, the major surface antigen of tachyzoite [Burg et al., 1988; Bülow and Boothroyd, 1991]. In contrast, previous work in our laboratory has highlighted the great interest of tachyzoite excreted-secreted antigens (ESAs) for a vaccine [Darcy et al., 1988; Decoster et al., 1988; Ridel et al., 1988; Duquesne et al., 1990; Darcy et al, 1992]. These ESAs have been found to be stored in the dense granules of Toxoplasma gondii.
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References
Achbarou, A., Mercereau-Puijalon, O., Sadak, A., Fortier, B., Leriche, M.A., Camus, D., and Dubremetz, J.F. (1991) Differential targeting of dense granule proteins in the parasitophorous vacuole of Toxoplasma gondii. Parasitol. 103: 321–329
Büllow, R. and Boothroyd, J.C. [ 1991 ] Protection of mice from fatal Toxoplasma gondii infection by immunization with P30 antigen in liposomes. J. Immunol. 147: 3496–3500.
Burg, J.L., Perelman, D., Kasper, L., Ware, P.L., and Boothroyd, J.C. [ 1988 ] Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J. Immunol. 141: 3584–3591.
Burn, B. [ 1988 ] Amphitropic proteins: a new class of membrane proteins. TIBS 13: 79–83.
Capron, A. and Dessaint, J.P. [ 1988 ] Vaccination against parasitic diseases: some alternative concepts for the definition of protective antigens. Ann. Inst. Past./Immunol. 139: 109–117.
Cesbron-Delauw, M.F., Guy, B., Torpier, G., Pierce, R.J., Lenzen, G., Cesbron, J.Y., Charif, H., Lepage, P., Darcy, F., Lecocq, J.P., and Capron, A. [ 1989 ] Molecular characterization of a 23-KDa major antigen secreted by Toxoplasma gondii. Proc. Natl. Acad. Sci. USA. 86: 7537–7541.
Cesbron-Delauw, M.F., Boutillon, C., Mercier, C., Fourmaux, M.P., Murray, A., Miquey, F., Tartar, A., and Capron, A. [ 1192 ] Amino acid sequence requirements for the epitope recognized by a monoclonal antibody reacting with the secreted antigen G.P.28.5 of Toxoplasma gondii. Mol. Chem. 29: 1375–1382.
Charif, H., Darcy, F., Torpier, G., Cesbron-Delauw, M.F., and Capron, A. [ 1990 ] Toxoplasma gondii: Characterization and localization of antigens secreted from tachyzoites. Exp. Parasitol. 71: 114–124.
Darcy, F., Deslée, D., Santoro, F., Charif, H., Auriault, C., Decoster, A., Duquesne, V., and Capron, A. [ 1988 ] Induction of a protective antibody-dependent response against toxoplasmosis by in vitro excreted- secreted antigens from tachyzoites of Toxoplasma gondii. Parasite Immunol. 10: 553–567.
Darcy, F., Charif, H., Caron, H., Deslée, D., Pierce, R.J., Cesbron-Delauw, M.F., Decoster, A., and Capron, A. [ 1990 ] Identification and biochemical characterization of antigens of tachyzoites and brayzoites of Toxoplasma gondii with cross-reactive epitopes. Parasitol. Res. 76: 473–478.
Darcy, F., Torpier, G., Cesbron-Delauw, M.F., Decoster, A. and Capron A. [ 1992 ] Diagnostic et prévention de la toxoplasmose: nouvelles approches et perspectives. Gynecol. Intern. 1: 48–57.
Decoster, A., Darcy, F., and Capron, A. [ 1988 ] Recognition of Toxoplasma gondii excreted and secreted antigens by human sera from acquired and congenital toxoplasmosis: identification of markers of acute and chronic infection. Clin. Exp. Immunol. 73: 376–382.
Dusquesne, V., Auriault, C., Darcy, F., Decavel, J.P., and Capron, A. [ 1990 ] Protection of nude rats against Toxoplasma infection by excreted- secreted antigens [ESA] specific helper T cells. Infect. Immun. 58: 2120–2126.
Dusquesne, V., Auriault, C., Gras-Masse, H., Boutillon, C., Darcy, F., Cesbron-Delauw, M.F., Tartar, A., and Capron, A. [ 1991 ] Identification of T-cell epitopes within a 23-kDa antigen (P24) of Toxoplasma gondii. Clin. Exp. Immunol. 84: 527–534.
Edman, P., and Begg, G., [ 1967 ] A protein sequenator. Eur. J. Biochem. 1: 80–91.
Gerke, V. [1991] p11, a member of the S-100 family, is associated with the tyrosin kinase substrate p36 (Annexinll) In: Novel calcium binding proteins. Fundamentals and clinical implications. (Heizmann, C.W., ed.) pp 139–155. Springer Verlag.
Kyte, J. and Doolittle R.F. [ 1982 ] A simple method for displaying the hydrophobic character of a protein. J. Mol. Biol. 157: 105–132.
Mercier, C., Lecordier, L., Darcy, F., Deslée, D., Murray, A., Tourvieille, B., Maes, P., Capron, A., and Cesbron-Delauw, M.F. (In Press) Toxoplasma gondii: molecular characterization of a dense granule antigen (GRA2) associated with the network of the parasitophorous vacuole. Molec. Biochem. Parasitol.
Mevelec, M.N., Chardes, T., Mercereau-Puijalon, O., Bourguin, I., Achbarou, A., Dubremetz, J.F., and Bout, D. [ 1992 ] Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein recognized by mucosal IgA antibodies. Molec. Biochem. Parasitol. 56: 227–238.
Nagel, S.D. and Boothroyd, J.C. [ 1988 ] The α- and β-tubulins of Toxoplasma gondii are encoded by simple copy genes containing multiple introns. Mol. Biochem. Parasitol. 29: 261–273.
Ossorio, P.N., Schwartzman, J.D., and Boothroyd, J.C. [ 1992 ] A Toxoplasma gondii rhoptry protein associated with host cell penetration has unusual charge asymetry. Mol. Biochem. Parasitol. 50: 1–16.
Parmley, S.F., Sgarlato, G.D. and Remington, J.S. (In Press) Genomic and corrected cDNA sequence of the P28 gene from Toxoplasma gondii. Mol. Biochem. Parasitol.
Prince, J.B., Araujo, F.G., Remington, J.S., Burg, L., Boothroyd, J.C., and Sharma, S.D. [ 1989 ] Cloning of cDNAs encoding a 28 kilodalton antigen of Toxoplasma gondii. Mol. Biochem. Parasitol. 34: 3–14.
Ridel, P.R., Auriault, C., Darcy, F., Pierce, R.J., Leite, P., Santoro, F., Neyrinck, J.L., Kusnierz, J.P., and Capron, A. (1988) Protective role of IgE in immunocompromised rat toxoplasmosis. J. Immunol. 141: 978–983.
Sharma, S.D., Araujo, F.G. and Remington, J.S., [1984] Toxoplasma antigen isolated by affinity chromatography with monoclonal antibody protects mice against lethal infection with Toxoplasma gondii. J. Immunol. 133: 2818–2820.
Sibley, L.D. and Sharma, S.D. [ 1987 ] Ultrastructural localization of an intracellular Toxoplasma protein that induces protection in mice. Infect. Immun. 55: 2137–2141.
Sibley, L.D., Pfefferkorn, E.R., and Boothroyd, J.C. [ 1991 ] Proposal for a uniform genetic nomenclature in Toxoplasma gondii. Parasitol. Today. 7: 327–328.
Sibley, L.D., and Boothroyd, J.C. [ 1992 ] Calcium-regulated secretion and modification of host-cell endocytic compartments by Toxoplasma. Abstract in J. Cell Biochem. Suppl. 16A: 163.
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© 1993 Springer-Verlag Berlin Heidelberg
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Cesbron-Delauw, MF., Mercier, C., Lecordier, L., Darcy, F., Capron, A. (1993). Dense Granule Antigens of Toxoplasma Gondii . In: Smith, J.E. (eds) Toxoplasmosis. NATO ASI Series, vol 78. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78559-7_4
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DOI: https://doi.org/10.1007/978-3-642-78559-7_4
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