The control experiments demonstrated a high specificity of the antibodies in the various stages of the immunohistochemical procedure. Sections of spinal cords from rats of different ages incubated with preimmune normal mouse serum or preimmune normal rabbit serum were found to be negative. Endogenous peroxidase could only be detected in erythrocytes in those animals that were not adequately, or not at all, perfused. Additionally, endogenous catalase activity could often be found in the pial blood vessels.
KeywordsDorsal Horn Ventral Horn Matrix Layer Mantle Layer Dorsal Root Entrance Zone
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