Chemical and Biological Approaches to Catalytic Antibodies
A long-standing goal of biochemists is the design and synthesis of catalysts endowed with particular activities and properties. For example, one might create an enzyme that can degrade drugs into inactive metabolites as a treatment for drug overdoses or an enzyme that acts on a prodrug to unmask its therapeutically useful form. Yet another enzyme could target the cleavage of a specific tumor or virus protein that is vital for development of that tumor or virus thus acting as a therapeutic agent. Other traits, such as thermostability, chemical stability, and oxidative stability, can also be incorporated into such catalysts.
KeywordsCatalysis Lipase Amide Proline Lysine
Unable to display preview. Download preview PDF.
- Baldwin J (1976) Rules for ring closure. J Chem Soc Chem Commun, pp 734–736Google Scholar
- Carter P, Kelley RF, Rodrigues ML, Snedecor B, Covarrubias M, Velligan MD, Wong WLT, Rowland AM, Kotts CE, Carver ME, Yang M, Bourell JH, Shepard HM, Henner D (1992) High level Escherichia coli expression and production of a bivalent humanized antibody fragment. Bio/Technology 10: 163–167PubMedCrossRefGoogle Scholar
- Jencks WP (1987) Catalysis in chemistry and enzymology. Dover MineolaGoogle Scholar
- Sastry L, Alting-Mees M, Huse WD, Short JM, Sorge JA, Hay BN, Janda KD, Benkovic SJ, Lerner RA (1989) Cloning of the immunological repertoire in Eschericia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific cDNA library. Proc Natl Acad Sci USA 86:5728–5732PubMedCrossRefGoogle Scholar
- Summers JB (1983) Catalytic principles of enzyme chemistry. PhD thesis, Harvard University, New HavenGoogle Scholar
- Summers MD, Smith GE (1987) A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station, College Station, TexasGoogle Scholar
- Taylor PJ (1972) The decarboxylation of some heterocyclic acetic acids. J Chem Soc, pp 1077–1086Google Scholar