Abstract
Several authors have described the isolation both in the peripheral blood and in tumor sites of human CTLs displaying various degrees of specificity against autologous malignant cells [1]. Some studies demonstrated the possibility of generating in vitro cytotoxic response specifically directed against autologous leukemia cells by using a pool of allogeneic peripheral blood mononuclear cells (PBMC) together with autologous leukemic blasts (LB) as stimulating cells [2,3]. The present study was undertaken to investigate the possible presence of circulating precursors T lymphocytes displaying cytotoxic activity against autologous LB and to assess suitable conditions for their in vitro activation and cloning.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Anichini A, Fossati G, Parmiani G (1987) Clonal analysis of the cytolitic T cell response to human tumors. Immunol Today 8: 386
Zarling JM, Raich PC, McKeough M, Bach FH (1976) Generation of cytotoxic lymphocytes in vitro against autologous human leukemia cells. Nature 262: 266
Zarling JM, Robins HI, Raich PC, Bach FH, Bach ML (1975) Generation of cytotoxic T lymphocytes to autologous human leukemia cells by sensitization to pooled allogeneic cells. Nature 274: 269
Rosenberg SA, Lotze MT, Muul LM, Leitman S, Chang AE, Ettinghausen SE, Matory YL, Skibber JM, Shiloni E, Vetto JT, Seipp CA, Simpson C, Reichert CM (1985) Observation on the systemic administration of autologous Iymphokine-activated killer cells and recombinant interleukin-2 to patients with metastatic cancer. N Engl J Med 313: 1485
Rosenberg SA (1986) Adoptive immunotherapy of cancer using Iymphokine activated killer cells and recombinant Interleukin-2, in De Vita VT, Hellman S, Rosenberg SA (eds): Important Advances in Oncology. Lippincott, NJ, p 55
Oshimi K, Oshimi Y, Akutsu M, Takei Y, Saito H, Okada M, Mizoguchi H (1986) Cytotoxicity of interleukin 2-activated lymphocytes for leukemia cells. Blood 68: 938
Fierro MT, Xin-Sheng L, Lusso P, Bonferroni M, Matera L, Cesano A, Arione R, Forni G, Foa R (1988) In vitro and in vivo susceptibility of human leukemic cells to Iymphokine activated killer activity. Leukemia 2: 50
var der Harst D, Brand A, van Luxemberg-Heys SAP, Kooy-Winkelaar EMC, van Rood JJ (1989) Lymphokine-activated killer cell functions in patients with leukemic B-Iymphoproliferative diseases. Blood 74: 2464
De Vries J, Spits H (1984) Cloned human cytotoxic T lymphocyte (CTL) lines reactive with autologous melanoma cells.I. In vitro generation, isolation, and analysis to phenotype and specificity. J Immunol 132: 510
Yessel H, Spits H, de Vries J (1984) A cloned human T cell line cytotoxic for autologous and allogeneic B lymphoma cells. J Exp Med 160: 239
Slovin SF, Lackman RD, Ferrone S, Kiely PE, Mastrangelo MJ (1986) Cellular immune response to human sarcomas: cytotoxic T cell clones reactive with autologous sarcomas.I. Development, phenotype, and specificity. J Immunol 137: 3042
Pawelec G, Reutter M, Owsianowsky M, Rehbein A, Busch FW (1991) Cytotoxic and noncytotoxic mechanisms involved in the in vitro anti-leukaemia effects of T cell clones established from a chronic myelogenous leukaemia patient during treatment in vivo with interferon. Cancer Immunol Immunother 33: 54
Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH (1986) The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 136: 4480
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Montagna, D., Aricò, M., Montini, E., De Benedetti, F., Martini, A., Maccario, R. (1994). In Vitro Isolation of Human Cytotoxic Clones Specific for Autologous Leukemic Blasts. In: Büchner, T., Hiddemann, W., Wörmann, B., Schellong, G., Ritter, J. (eds) Acute Leukemias IV. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 36. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78350-0_21
Download citation
DOI: https://doi.org/10.1007/978-3-642-78350-0_21
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-78352-4
Online ISBN: 978-3-642-78350-0
eBook Packages: Springer Book Archive