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Functional Expression of Mammalian Receptors and G-Proteins in Yeast

  • K. J. Blumer
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 108 / 2)

Abstract

Biologists studying G-protein-linked signal transduction pathways have used heterologous expression systems to make many important discoveries, including the molecular description of pharmacologically distinct subtypes of receptors, and the specificity with which receptors, G-proteins, and their effectors interact with one another. However, commonly used expression systems, including those employing Xenopus oocytes or transfected mammalian cell lines, pose limitations for basic and applied scientists alike. Such systems are not usually amenable to rigorous formal genetic analysis of signal transduction pathways, and they are costly to use as biological screening tools in drug discovery programs.

Keywords

Muscarinic Acetylcholine Receptor Pheromone Receptor Pheromone Response Mating Pheromone Ligand Binding Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • K. J. Blumer

There are no affiliations available

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