Functional Expression of Mammalian Receptors and G-Proteins in Yeast
Biologists studying G-protein-linked signal transduction pathways have used heterologous expression systems to make many important discoveries, including the molecular description of pharmacologically distinct subtypes of receptors, and the specificity with which receptors, G-proteins, and their effectors interact with one another. However, commonly used expression systems, including those employing Xenopus oocytes or transfected mammalian cell lines, pose limitations for basic and applied scientists alike. Such systems are not usually amenable to rigorous formal genetic analysis of signal transduction pathways, and they are costly to use as biological screening tools in drug discovery programs.
KeywordsMuscarinic Acetylcholine Receptor Pheromone Receptor Pheromone Response Mating Pheromone Ligand Binding Activity
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