Functional Expression of Mammalian Receptors and G-Proteins in Yeast
Biologists studying G-protein-linked signal transduction pathways have used heterologous expression systems to make many important discoveries, including the molecular description of pharmacologically distinct subtypes of receptors, and the specificity with which receptors, G-proteins, and their effectors interact with one another. However, commonly used expression systems, including those employing Xenopus oocytes or transfected mammalian cell lines, pose limitations for basic and applied scientists alike. Such systems are not usually amenable to rigorous formal genetic analysis of signal transduction pathways, and they are costly to use as biological screening tools in drug discovery programs.
KeywordsCysteine Fractionation Proline Norepinephrine Disulfide
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- Buckley NJ, Bonner TI, Buckley CM, Brann MR (1989) Antagonist binding proterties of five cloned muscarinic receptors expressed in CHO-K1 cells. Mol Pharm 35:469–476.Google Scholar
- Nakafuku M, Obara K, Kaibuchi K, Miyajima I, Miyajima A, Itoh H, Nakamura S, Arai K-I, Matsumoto K, Kaziro Y (1988) Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein: studies on its structure and possible functions. Proc Natl Acad Sci USA 85:1374–1378.PubMedCrossRefGoogle Scholar