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Complementation of the Cellular A-T Phenotype by Gene Transfer

  • Conference paper
Ataxia-Telangiectasia

Part of the book series: NATO ASI Series ((ASIH,volume 77))

Abstract

The identification of the genes responsible for the chromosomal breakage syndromes by their function has been discussed since the discovery of the common feature of these diseases, cellular sensitivity to specific DNA damaging agents. The advent of gene transfer methods supplied the experimental system for this approach, which is apparently simple and straightforward: Exogenous DNA is introduced in vitro into the patient’s cells, and selection is applied to identify cell clones showing increased resistance to the lethal action of the DNA damaging agent. An attempt is then made to rescue the piece of DNA responsible for this phenotypic change, which is expected to represent a candidate gene.

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Author information

Authors and Affiliations

  1. Department of Human Genetics Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel

    Yael Ziv, Tsafi Danieli, Galit Rotman, Adam Sartiel, Anat Bar-Shira & Yosef Shiloh

  2. Department of Cell Biology and Genetics, Erasmus University, 3000 DR, Rotterdam, Netherlands

    N. G. J. Jaspers

  3. Department of Biological Sciences, Stanford University, Stanford, CA, 94305-5020, USA

    Richard Swirski & Robert T. Schimke

  4. Department of Human Genetics, Roswell Park Cancer Institute, Buffalo, NY, 14263, USA

    Roger L. Eddy & Thomas B. Shows

Authors
  1. Yael Ziv
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  2. Tsafi Danieli
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  3. Galit Rotman
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  4. Adam Sartiel
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  5. Anat Bar-Shira
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  6. N. G. J. Jaspers
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  7. Richard Swirski
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  8. Robert T. Schimke
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  9. Roger L. Eddy
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  10. Thomas B. Shows
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  11. Yosef Shiloh
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Editor information

Editors and Affiliations

  1. Department of Pathology, University of California Los Angeles School of Medicine, Los Angeles, CA, 90024-6975, USA

    Richard A. Gatti

  2. Laboratory of Radiobiology and Environmental Health, University of California San Francisco, San Francisco, CA, 94143-0750, USA

    Robert B. Painter

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© 1993 Springer-Verlag Berlin Heidelberg

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Ziv, Y. et al. (1993). Complementation of the Cellular A-T Phenotype by Gene Transfer. In: Gatti, R.A., Painter, R.B. (eds) Ataxia-Telangiectasia. NATO ASI Series, vol 77. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78278-7_6

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  • DOI: https://doi.org/10.1007/978-3-642-78278-7_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78280-0

  • Online ISBN: 978-3-642-78278-7

  • eBook Packages: Springer Book Archive

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