Advertisement

The Biology of ADP-Ribosylation Factors

  • R. A. Kahn
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 108 / 1)

Abstract

Adenosine diphosphate (ADP) ribosylation factor (ARF) was originally defined as an activity required for the efficient activation of the purified, stimulatory, regulatory component of adenylyl cyclase, Gs, by cholera toxin (Schleifer et al. 1982; Kahn 1989, 1991). The toxin catalyzes the covalent addition of the ADP-ribose moiety of nicotinamide adenine dinucleotide (NAD) onto the α subunit of Gs. The consequences of this modification include decreased intrinsic GTP hydrolysis by the Gs protein (Cassel and Selinger 1979) and its consequent activation and subunit dissociation (Kahn and Gilman 1984a). Thus, while GTP alone is normally a very weak stimulator of membrane cyclase, after ADP-ribosylation GTP is nearly as potent as the slowly hydrolyzable analogs of GTP, e. g., GTPγS or Gpp(NH)p.

Keywords

Adenylate Cyclase Cholera Toxin Nicotinamide Adenine Dinucleotide Golgi Membrane Nucleoside Diphosphate Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alsip GR, Konkel DA (1986) A processed chicken pseudogene (CPS1) related to the ras oncogene superfamily. Nucleic Acids Res 14:2123–2138PubMedCrossRefGoogle Scholar
  2. Balch WE, Kahn RA, Schwaninger R (1992) ADP-ribosylation factor (ARF) is required for vesicular trafficking between the endoplasmic reticulum (ER) and the cis Golgi compartment. J Biol Chem 267:13053–13061PubMedGoogle Scholar
  3. Beckers CJM, Balch WE (1989) Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and golgi apparatus. J Cell Biol 108:1245–1256PubMedCrossRefGoogle Scholar
  4. Bobak DA, Nightingale MS, Murtagh JJ, Price SR, Moss J, Vaughan M (1989) Molecular cloning, characterization, and expression of human ADP-ribosylation factors: two guanine nucleotide-dependent activators of cholera toxin. Proc Natl Acad Sci USA 86:6101–6105PubMedCrossRefGoogle Scholar
  5. Botstein D, Segev N, Stearns T, Hoyt MA, Holden J, Kahn RA (1988) Diverse biological functions of small GTP-binding proteins in yeast. Cold Spring Harb Symp Quant Biol 53:629–636PubMedCrossRefGoogle Scholar
  6. Cassel D, Selinger Z (1979) Mechanism of adenylate cyclase activation by cholera toxin: inhibition of GTP hydrolysis at the regulatory site. Proc Natl Acad Sci USA 74:3307–3311CrossRefGoogle Scholar
  7. Cavenagh MM, Kahn RA (1993) Requirement for ARF in the sporulation process of S. cerevisiae (in preparation)Google Scholar
  8. Donaldson JG, Kahn RA, Lippincott-Schwartz J, Klausner RD (1991) Binding of the BFA-sensitive proteins ARF and B-COP to Golgi membranes: involvement of a trimeric G protein. Science 254:1197–1199PubMedCrossRefGoogle Scholar
  9. Donaldson JG, Cassel D, Kahn RA, Klausner RD (1992) ADP-ribosylation factor, a small GTP binding protein, is required for binding of the coatomer protein β-COP to Golgi membranes. Proc Natl Acad Sci USA 89:6408–6412PubMedCrossRefGoogle Scholar
  10. Duronio RJ, Jackson-Machelski E, Heuckeroth RO, Olins PO, Devine CS, Yonemoto W, Slice LW, Taylor SS, Gordon JI (1990) Protein N-myristoylation in Escherichia coli: reconstitution of a eukaryotic protein modification in bacteria. Proc Natl Acad Sci USA 87:1506–1510PubMedCrossRefGoogle Scholar
  11. Kahn RA (1989) The ADP-ribosylation factor of adenylate cyclase: a 21kDa GTP-binding protein. In: Birnbaumer L, Iyengar R (eds) G-Proteins. Academic, OrlandoGoogle Scholar
  12. Kahn RA (1991) Quantitation and purification of ADP-ribosylation factor. In: Johnson RA, Corbin JD (eds) Methods in enzymology, vol 195. Academic, OrlandoGoogle Scholar
  13. Kahn RA, Gilman AG (1984a) Effects of cholera toxin-dependent ADP-ribosylation on the regulatory component of adenylate cyclase. J Biol Chem 259:6235–6240PubMedGoogle Scholar
  14. Kahn RA, Gilman AG (1984b) Purification of a protein cofactor required for ADP-ribosylation of the stimulatory regulatory component of adenylate cyclase by cholera toxin. J Biol Chem 259:6228–6234PubMedGoogle Scholar
  15. Kahn RA, Gilman AG (1986) The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP-binding protein. J Biol Chem 261:7906–7911PubMedGoogle Scholar
  16. Kahn RA, Rulka C (1992) Association of a GTP-binding protein (ARF) with phospholipid vesicles is controlled by guanine nucleotide binding. J Biol Chem (submitted)Google Scholar
  17. Kahn RA, Goddard C, Newkirk M (1988) Chemical and immunological characterization of the 21kDa ADP-ribosylation factor (ARF) of adenylate cyclase. J Biol Chem 263:8282–8287PubMedGoogle Scholar
  18. Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991) Human ADP-ribosylation factors: a functionally conserved family of GTP-binding proteins. J Biol Chem 266:2606–2614PubMedGoogle Scholar
  19. Kahn RA, Der CJ, Bokoch GM (1992a) The ras superfamily of GTP-binding proteins: guidelines on nomenclature. FASEB J 6:2512–2513PubMedGoogle Scholar
  20. Kahn RA, Randazzo PA, Serafini T, Weiss O, Rulka C, Clark J, Amherdt M, Roller PP, Orci L, Rothman JE (1992b) The amino terminus of ADP ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport. J Biol Chem 267:13039–13046PubMedGoogle Scholar
  21. Lenhard JM, Kahn RA, Stahl PD (1992) Evidence for ADP-ribosylation factor (ARF) as a regulator of in vitro endosome-endosome fusion. J Biol Chem 267:13047–13052PubMedGoogle Scholar
  22. Melancon P, Glick BS, Malhotra V, Weidman PJ, Serafina T, Gleason ML, Orci L, Rothman JE (1987) Involvement of GTP-binding “G” proteins in transport through the Golgi stack. Cell 51:1053–1062PubMedCrossRefGoogle Scholar
  23. Misumi Y, Miki K, Takatsuki A, Tamura G, Ikehara Y (1986) Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes. J Biol Chem 261:11398–11403PubMedGoogle Scholar
  24. Monaco L, Murtagh JJ, Newman KB, Tsai S, Moss J, Vaughan M (1990) Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin. Proc Natl Acad Sci USA 87:2206–2210PubMedCrossRefGoogle Scholar
  25. Nakano A, Muramatsu M (1989) A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. J Cell Biol 109:2677–2691PubMedCrossRefGoogle Scholar
  26. Pelham HRB (1991) Multiple targets for Brefeldin A. Cell 67:449–451PubMedCrossRefGoogle Scholar
  27. Peng Z, Calver I, Clark J, Helman L, Kahn RA, Kung H (1989) Molecular cloning, sequence analysis and mRNA expression of human ADP-ribosylation factor. Biofactors 2:45–49PubMedGoogle Scholar
  28. Price SR, Nightingale M, Tsai S, Williamson KC, Adamik R, Chen H, Moss J, Vaughan M (1988) Guanine nucleotide-binding proteins that enhance choleragen ADP-ribosyltransferase activity: nucleotide and deduced amino acid sequence of an ADP-ribosylation factor cDNA. Proc Natl Acad Sci USA 85:5488–5491PubMedCrossRefGoogle Scholar
  29. Randazzo PA, Northup JK, Kahn RA (1991) Activation of a small GTP binding protein by nucleoside diphosphate kinase. Science 254:850–853PubMedCrossRefGoogle Scholar
  30. Randazzo PA, Northup JK, Kahn RA (1992) Regulatory GTP binding proteins (ARF, Gt, and RAS) are not activated directly by nucleoside diphosphate kinase. J Biol Chem 267:18182–18189PubMedGoogle Scholar
  31. Regazzi R, Ullrich S, Kahn RA, Wollheim CB (1991) Redistribution of ADP-ribosylation factor during stimulation of permeabilized cells with GTP analogues. Biochem J 275:639–644PubMedGoogle Scholar
  32. Schleifer LS, Kahn RA, Hanski E, Northup JK, Sternweis PC, Gilman AG (1982) Requirements for cholera toxin-dependent ADP-ribosylation of the purified regulatory component of adenylate cyclase. J Biol Chem 257:20–23PubMedGoogle Scholar
  33. Serafini T, Orci L, Amnerdt M, Brunner M, Kahn RA, Rothman JE (1991) ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP binding protein. Cell 67:239–254PubMedCrossRefGoogle Scholar
  34. Sewell JL, Kahn RA (1988) Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins. Proc Natl Acad Sci USA 85:4620–4624PubMedCrossRefGoogle Scholar
  35. Stearns T, Hoyt MA, Botstein D, Kahn RA (1990a) ADP-ribosylation factor is an essential protein in yeast and is encoded by two genes. Mol Cell Biol 10:6690–6699PubMedGoogle Scholar
  36. Stearns T, Willingham MC, Botstein D, Kahn RA (1990b) The ADP-ribosylation factor is functionally and physically associated with the Golgi complex. Proc Natl Acad Sci USA 87:1238–1242PubMedCrossRefGoogle Scholar
  37. Tamkun JW, Kahn RA, Kissinger M, Brizuela BJ, Rulka C, Scott MP, Kennison JA (1991) The arf-like gene encodes an essential GTP-binding protein in Drosophila. Proc Natl Acad Sci USA 88:3120–3124PubMedCrossRefGoogle Scholar
  38. Taylor TC, Kahn RA, Melancon P (1992) Two distinct members of the ADP-ribosylation factor family of GTP binding proteins regulate cell free intra-Golgi transport. Cell 70:69–79PubMedCrossRefGoogle Scholar
  39. Towler DA, Eubanks SR, Towery DS, Adams SP, Glaser L (1987) Amino-terminal processing of proteins by N-myristoylation. J Biol Chem 262:1030–1036PubMedGoogle Scholar
  40. Tsuchiya M, Price SR, Tsai S-C, Moss J, Vaughan M (1991) Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells. J Biol Chem 266:2772–2777PubMedGoogle Scholar
  41. Weiss O, Holden J, Rulka C, Kahn RA (1989) Nucleotide binding and cofactor activities of purified bovine brain and bacterially expressed ADP-ribosylation factor (ARF). J Biol Chem 264:21066–21072PubMedGoogle Scholar
  42. Zhang C-J, Clark J, Rulka C, Kahn RA (1993) Mutational analysis of yeast ARF1 (in preparation)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • R. A. Kahn

There are no affiliations available

Personalised recommendations