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GTPases in Biology I pp 487–505Cite as

Regulated Exocytosis and Interorganelle Vesicular Traffic: A Comparative Analysis

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Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 108 / 1))

Abstract

There are two main pathways of vesicular protein traffic and secretion in eukaryotic cells. One, common to all cells, is constitutive. The other, termed regulated, is present in specialized cell types and serves to rapidly export selected proteins under controlled conditions (Burgess and Kelly 1987). During transit towards the cell surface, all secretory proteins share a number of constitutive vesicular transport steps through the endoplasmic reticulum (ER) and the Golgi compartments. Their fates then diverge in the tract from the trans Golgi network (TGN) to the plasma membrane in two major respects. Proteins secreted by the regulated pathway are sorted, concentrated, and stored for variable periods of time in rather large specialized structures, the secretory granules. By contrast, constitutively secreted proteins are enclosed in small vesicles (50–60 nm in diameter), similar to those used in intra-Golgi transport, and flowing continuously towards the plasma membrane where they fuse. The second difference is that exocytosis of regulated granules occurs only upon triggering by an appropriate extracellular signal, whereas all other transport steps proceed continuously and, in fact, constitutively.

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References

  • Ahnert-Hilger G, Bräutigam M, Gratzl M (1987) Ca2+-stimulated catecholamine release from α-toxin-permeabilized PC12 cells: biochemical evidence for exocytosis and its modulation by protein kinase C and G proteins. Biochemistry 26:7842–7848

    PubMed  CAS  Google Scholar 

  • Aridor M, Traub LM, Sagi-Eisenberg R (1990) Exocytosis in mast cells by basic secretagogues: evidence for direct activation of GTP-binding proteins. J Cell Biol 111:909–917

    PubMed  CAS  Google Scholar 

  • Balch WE (1990) Small GTP-binding proteins in vesicular transport. Trends Biochem Sci 15:473–477

    PubMed  Google Scholar 

  • Barr FA, Leyte A, Mollner S, Pfeuffer T, Tooze SA, Huttner WB (1991) Trimeric G-proteins of the trans-Golgi network are involved in the formation of constitutive secretory vesicles and immature secretory granules. FEBS Lett 294:239–243

    PubMed  CAS  Google Scholar 

  • Barr FA, Leyte A, Huttner WB (1992) Trimeric G proteins and vesicle formation. Trends Cell Biol 2:91–94

    PubMed  CAS  Google Scholar 

  • Barrowman MM, Cockroft S, Gomperts BD (1986) Two roles for guanine nucleotides in the stimulus-secretion sequence of neutrophils. Nature 319:504–507

    PubMed  CAS  Google Scholar 

  • Beckers CJM, Balch WE (1989) Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus. J Cell Biol 108:1245–1256

    PubMed  CAS  Google Scholar 

  • Bittner MA, Holz RW, Neubig RR (1986) Guanine nucleotides effects on cathecolamine secretion from digitonin-permeabilized adrenal chromaffin cells. J Biol Chem 261:10182–10188

    PubMed  CAS  Google Scholar 

  • Boman AL, Delannoy MR, Wilson KL (1992) GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro. J Cell Biol 116:281–294

    PubMed  CAS  Google Scholar 

  • Bourne HR, Sanders DA, McCormick F (1991) The GTPase superfamily: conserved structure and molecular mechanism. Nature 349:117–127

    PubMed  CAS  Google Scholar 

  • Burgess TL, Kelly RB (1987) Constitutive and regulated secretion of proteins. Annu Rev Cell Biol 3:243–293

    PubMed  CAS  Google Scholar 

  • Burgoyne RD, Morgan A (1989) Low molecular mass GTP-binding proteins of adrenal chromaffin cells are present on the secretory granule. FEBS Lett 245:122–126

    PubMed  CAS  Google Scholar 

  • Chavrier P, Parton RG, Hauri HP, Simons K, Zerial M (1990) Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments. Cell 62:317–329

    PubMed  CAS  Google Scholar 

  • Colombo MI, Mayorga LS, Casey PJ, Stahl PD (1992) Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion. Science 255:1695–1697

    PubMed  CAS  Google Scholar 

  • Darchen F, Zarhoui A, Hammel F, Montelis MP, Tavitian A, Scherman D (1990) Association of the GTP-binding protein Rab3A with bovine adrenal chromaffin granules. Proc Natl Acad Sci USA 87:5692–5696

    PubMed  CAS  Google Scholar 

  • Davidson HW, McGowan CH, Balch WE (1992) Evidence for the regulation of exocytic transport by protein phosphorylation. J Cell Biol 116:1343–1355

    PubMed  CAS  Google Scholar 

  • Davis BH, Walter RJ, Pearson CB, Becker EL, Oliver JM (1982) Membrane activity and topography of f-Met-Leu-Phe-treated polymorphonuclear leukocytes. Acute and sustained responses to chemotactic peptide. Am J Pathol 108:206–216

    PubMed  CAS  Google Scholar 

  • de Curtis I, Simons K (1988) Dissection of Semliki Forest virus glycoprotein delivery from the trans-Golgi network to the cell surface in permeabilized BHK cells. Proc Natl Acad Sci USA 85:8052–8056

    PubMed  Google Scholar 

  • De Matteis MA, Di Tullio G, Buccione R, Luini A (1991) Characterization of calcium-triggered secretion in permeabilized rat basophilic leukemia cells. Possible role of vectorially acting G proteins. J Biol Chem 266:10452–10460

    PubMed  Google Scholar 

  • De Matteis MA, Santini G, Kahn RA, Di Tullio G, Luini A (1993) Receptor and protein kinasi C regulation of ARF binding to the Golgi complex. Nature 384:818–821

    Google Scholar 

  • d’Enfert C, Wuestehube LJ, Lila T, Schekman R (1991) Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER. J Cell Biol 114:663–670

    CAS  Google Scholar 

  • Dexter D, Rubins JB Manning EC, Khachatrian L, Dickey BF (1990) Compartmentalization of low molecular mass GTP-binding proteins among neutrophil secretory granules. J Immunol 145:1845–1850

    PubMed  CAS  Google Scholar 

  • Donaldson JG, Kahn RA, Lippincott-Schwartz J, Klausner RD (1991) Binding of ARF and β-COP to Golgi membranes: possible regulation by a trimeric G protein. Science 254:1197–1199

    PubMed  CAS  Google Scholar 

  • Ercolani L, Stow JL, Boyle JF, Holtzman EJ, Lin H, Grove JR, Ausiello DA (1990) Membrane localization of the pertussis toxin-sensitive G-protein subunits αi-2 and αi-3 and expression of a metallothionein-αi-2 fusion gene in LLC-PK1 cells. Proc Natl Acad Sci USA 87:4637–4639

    Google Scholar 

  • Fischer von Mollard G, Mignery G, Baumert M, Perin M, Hanson T, Jahn R, Sudhof T (1990) Rab 3 is a small GTP-binding protein exclusively localized to synaptic vesicles. Proc Natl Acad Sci USA 87:1988–1992

    Google Scholar 

  • Fischer von Mollard G, Südhof TC, Jahn R (1991) A small GTP-binding protein dissociates from synaptic vesicles during exocytosis. Nature 349:79–81

    Google Scholar 

  • Furuichi K, Rivera J, Isersky C (1984) The fate of IgE bound to rat basophilic leukemia cells. III. Relationship between antigen-induced endocytosis and serotonin release. J Immunol 133:1513–1520

    PubMed  CAS  Google Scholar 

  • Gallwitz D, Donath C, Sander C (1983) A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product. Nature 306:704–707

    PubMed  CAS  Google Scholar 

  • Gilman AG (1987) G-proteins: transducers of receptor-generated signals. Annu Rev Biochem 56:615–649

    PubMed  CAS  Google Scholar 

  • Goda Y, Pfeffer SR (1988) Selective recycling of the mannose 6-phosphate/IGF-II receptor to the trans Golgi network in vitro. Cell 55:309–320

    PubMed  CAS  Google Scholar 

  • Gomperts BD (1983) Involvement of guanine-nucleotide binding protein in the gating of Ca2+ by receptors. Nature 306:64–66

    PubMed  CAS  Google Scholar 

  • Gomperts BD (1990) Ge: a GTP-binding protein mediating exocytosis. Annu Rev Physiol 52:591–606

    PubMed  CAS  Google Scholar 

  • Gorvel J-P, Chavrier P, Zerial M, Gruenberg J (1991) Rab5 controls early endosome fusion in vitro. Cell 64:915–925

    PubMed  CAS  Google Scholar 

  • Goud B, McCaffrey M (1991) Small GTP-binding proteins and their role in transport. Curr Opin Cell Biol 3:626–633

    PubMed  CAS  Google Scholar 

  • Grimes M, Kelly RB (1992) Intermediates in the constitutive and regulated secretory pathways released in vitro from semi-intact cells. J Cell Biol 11:539–549

    Google Scholar 

  • Haigler HT, McKanna JA, Cohen S (1979) Rapid stimulation of pinocytosis in human carcinoma cells A-431 by epidermal growth factor. J Cell Biol 83:82–90

    PubMed  CAS  Google Scholar 

  • Haraguchi K, Rodbell M (1990) Isoproterenol stimulates shift of G proteins from plasma membrane to pinocytic vesicles in rat adipocytes: a possible means of signal dissemination. Proc Natl Acad Sci USA 87:1208–1212

    PubMed  CAS  Google Scholar 

  • Haraguchi K, Rodbell M (1991) Carbachol-activated muscarinic (M1 and M3) receptors transfected into Chinese hamster ovary cells inhibit trafficking of endosomes. Proc Natl Acad Sci USA 88:5964–5968

    PubMed  CAS  Google Scholar 

  • Helms JB, Karrenbauer A, Wirtz WA, Rothman JE, Wieland FT (1990) Reconstitution of steps in the constitutive secretory pathway in permeabilized cells. J Biol Chem 265:20027–20032

    PubMed  CAS  Google Scholar 

  • Higashijiman T, Uzu S, Nakajima T, Ross EM (1988) Mastoparan, a peptide toxin from wasp venom, mimics receptors by activating. GTP-binding regulatory proteins (G proteins). J Biol Chem 263:6491–6494

    Google Scholar 

  • Howell TW, Cockroft S, Gomperts BD (1987) Essential synergy between Ca2+ and guanine nucleotides in exocytotic secretion from permeabilized rat mas cells. J Cell Biol 105:191–197

    PubMed  CAS  Google Scholar 

  • Kahn RA (1991) Fluoride is not an activator of the smaller (20–25 kDa) GTP-binding proteins. J Biol Chem 266:15595–15597

    PubMed  CAS  Google Scholar 

  • Knight DE, Baker PF (1985) Guanine nucleotides and Ca-dependent exocytosis. FEBS Lett 189:345–349

    PubMed  CAS  Google Scholar 

  • Knight DE, Scrutton MC (1986) Effects of guanine nucleotides on the properties of 5-hyroxytryptamine secretion from electropermeabilised human platelets. Eur J Biochem 160:183–190

    PubMed  CAS  Google Scholar 

  • Koopmann WR Jr, Jackson RC (1990) Calcium-and guanine-nucleotide-dependent exocytosis in permeabilized rat mast cells. Biochem J 265:365–373

    PubMed  CAS  Google Scholar 

  • Ktistakis NT, Linder ME, Roth MG (1992) Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 356:344–346

    PubMed  CAS  Google Scholar 

  • Lenhard JM, Kahn RA, Stahl PD (1992) Evidence for ADP-ribosylation factor (ARF) as a regulator of in vitro endosome-endosome fusion. J Biol Chem 267:13047–13052

    PubMed  CAS  Google Scholar 

  • Li GD, Regazzi R, Balch WE, Wollheim CB (1992) An effector domain peptide of GTP binding protein rab 3 stimulates insulin release from permeabilized HIT cells. Diabetologia 35a

    Google Scholar 

  • Lindau M, Gomperts BD (1991) Techniques and concepts in exocytosis: focus on mast cells. Biochem Biophys Acta 1071:429–471

    PubMed  CAS  Google Scholar 

  • Lledo PM, Vernier P, Vincent JD, William TM, Zorec R (1993) Inhibition of RAB3 expression attenuates Ca2+-dependent exocytosis in rat anterior pituitary cells. Nature 364:540–544

    PubMed  CAS  Google Scholar 

  • Luini A, De Matteis MA (1988) Dual regulation of ACTH secretion by guanine nucleotides in permeabilized AtT-20 cells. Cell Mol Neurobiol 8:129–138

    PubMed  CAS  Google Scholar 

  • Luini A, De Matteis MA (1990) Evidence that receptor-linked G protein inhibits exocytosis by a post-second messenger mechanism in AtT-20 cells. J Neurochem 54:30–38

    PubMed  CAS  Google Scholar 

  • Malhotra V, Serafini T, Orci L, Shepherd JC, Rothman JE (1989) Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack. Cell 58:329–336

    PubMed  CAS  Google Scholar 

  • Maridonneau-Parini I, de Gunzburg J (1992) Association of rap1 and rap2 proteins with the specific granules of human neutrophils. J Biol Chem 267:6396–6402

    PubMed  CAS  Google Scholar 

  • Matteoli M, Takei K, Cameron R, Hurlbut P, Johnston PA, Sudhof TC, Jahn R, De Camilli P (1991) Association of rab3a with synaptic vesicles at late stages of the secretory pathway. J Cell Biol 115:625–633

    PubMed  CAS  Google Scholar 

  • Mayorga LS, Diaz R, Stahl PD (1989) Regulatory role for GTP-binding proteins in endocytosis. Science 244:1475–1477

    PubMed  CAS  Google Scholar 

  • Melançon P, Glick BJ, Malhotra V, Weidman PJ, Serafini T, Gleason ML, Orci L, Rothman JE (1987) Involvement of GTP-binding “G” proteins in transport through the Golgi stack. Cell 51:1053–1062

    PubMed  Google Scholar 

  • Melançon P, Franzusoff A, Howell KE (1991) Vesicle budding: insights from cell-free assays. Trends Cell Biol 1:165–171

    PubMed  Google Scholar 

  • Miller SG, Moore H-P (1991) Reconstitution of constitutive secretion using semi-intact cells: regulation by GTP but not calcium. J Cell Biol 112:39–54

    PubMed  CAS  Google Scholar 

  • Mizoguchi A, Kim S, Ueda T, Kikuchi A, Yorifuji H, Hirokawa N, Takai Y (1990) Localization and subcellular distribution of smg p25A, a ras p21-like GTP-binding protein, in rat brain. J Biol Chem 265:11872–11879

    PubMed  CAS  Google Scholar 

  • Morgan A, Burgoyne RD (1990) Stimulation of Ca2+-independent catecholamine secretion from digitonin-permeabilized bovine adrenal chromaffin cells by guanine nucleotide analogues. Biochem J 269:521–526

    PubMed  CAS  Google Scholar 

  • Mousli M, Bronner C, Bueb J-L, Tschirhart E, Gies J-P, Landry Y (1989) Activation of rat peritoneal mast cells by substance P and mastoparan. J Pharmacol Exp Ther 250:329–335

    PubMed  CAS  Google Scholar 

  • Nadin CY, Rogers J, Tomlinson S, Edwardson JM (1989) A specific interaction in vitro between pancreatic zymogen granules and plasma membranes: stimulation by G protein activators but not by Ca2+. J Cell Biol 109:2801–2808

    PubMed  CAS  Google Scholar 

  • Nakamura T, Ui M (1985) Simultaneous inhibitions of inositol phospholopid breakdown, arachidonic acid release, and histamine secretion in mast cells by islet-activating protein, pertussis toxin. A possible involvement of the toxin-specific substrate in the Ca2+-mobilizing receptor-mediated biosignalling system. J Biol Chem 260:3584–3593

    PubMed  CAS  Google Scholar 

  • Oberhauser AF, Monck JR, Balch WE, Fernandez JM (1992) Exocytotic fusion is directly activated by rab 3 AL peptides. Nature 360:270–273

    PubMed  CAS  Google Scholar 

  • Ohara-Imaizumi M, Kameyama K, Kawae N, Takeda K, Muramatsu S, Kumakura K (1992) Regulatory role of the GTP-binding protein, Go, in the mechanism of exocytosis in adrenal chromaffin cells. J Neurochem 58:2275–2284

    PubMed  CAS  Google Scholar 

  • Orci L, Tagaya M, Amherdt M, Perrelet A, Donaldson JG, Lippincott-Schwartz J, Klausner RD, Rothman JE (1991) Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae. Cell 64:1183–1195

    PubMed  CAS  Google Scholar 

  • Ozaki Y, Matsumoto Y, Yatomi Y, Higashihara M, Kariya T, Kume S (1990) Mastoparan, a wasp venom, activates platelets via pertussis toxin-sensitive GTP-binding proteins. Biochem Biophys Res Commun 170:779–785

    PubMed  CAS  Google Scholar 

  • Padfield PJ, Ding T-G, Jamieson JD (1991) Ca2+-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins. Biochem Biophys Res Commum 174:536–541

    CAS  Google Scholar 

  • Padfield PJ, Balch WE, Jamieson JP (1992) A synthetic peptide of the rab 3a effector domain stimulates amylase release from permeabilized pancreatic acini. Proc Natl Acad Sci USA 89:1656–1660

    PubMed  CAS  Google Scholar 

  • Pagano RE (1988) What is the fate of diacylglycerol produced at the Golgi apparatus? Trends Biochem Sci 13:202–205

    PubMed  CAS  Google Scholar 

  • Peterson JB (1991) Small GTP-binding proteins associated with secretory vesicles of Paramecium. J Protozool 38:495–501

    PubMed  CAS  Google Scholar 

  • Pfeffer SR (1992) GTP-binding proteins in intracellular transport. Trends Cell Biol 2:41–46

    PubMed  CAS  Google Scholar 

  • Pfeiffer JR, Seagrave JC, Davis BH, Deanin GG, Oliver JM (1985) Membrane and cytoskeletal changes associated with IgE-mediated serotonin release from rat basophilic leukemia cells. J Cell Biol 101:2145–2155

    PubMed  CAS  Google Scholar 

  • Philips MR, Abramson SB, Kolasinski SL, Haines KA, Weissmann G, Rosenfeld MG (1991) Low molecular weight GTP-binding proteins in human neutrophil granule membrane. J Biol Chem 266:1289–1298

    PubMed  CAS  Google Scholar 

  • Plutner H, Schwaninger R, Pind S, Balch WE (1990) Synthetic peptides of the Rab effector domain inhibit vesicular transport through the secretory pathway. EMBO J 9:2375–2383

    PubMed  CAS  Google Scholar 

  • Plutner H, Cox AD, Pind S, Khosravi-Far R, Bourne JR, Schwaninger R, Der CJ, Balch WE (1991) Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. J Cell Biol 115:31–43

    PubMed  CAS  Google Scholar 

  • Rennison ME, Kerr MA, Addey CPV, Wilde CJ, Burgoyne RD (1992) Inhibition of constitutive protein secretion from alctating mouse mammary epithelial cells by FIL (feedback inhibitor of lactation), a secreted milk protein (submitted)

    Google Scholar 

  • Rexach MF, Schekman RW (1991) Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles. J Cell Biol 114:219–229

    PubMed  CAS  Google Scholar 

  • Rotrosen D, Gallin JI, Spiegel AM, Malech HL (1988) Subcellular localization of Giα in human neutrophils. J Biol Chem 263:10958–10964

    PubMed  CAS  Google Scholar 

  • Rudolph HK, Antebi A, Fink GR, Buckley CM, Dorman TE, LeVitre J, Davidow LS, Mao DT (1989) The yeast secretory pathway is perturbed by mutations in PMR1, a Ca2+ ATPase family. Cell 58:133–145

    PubMed  CAS  Google Scholar 

  • Saito H, Okajima F, Molski TFP, Sha’Afi RI, Ui M, Ishizaka T (1987) Effects of ADP-ribosylation of GTP-binding protein by pertussis toxin on immunoglobulin E-dependent and-independent histamine release from mast cells and basophils. J Immunol 138:3927–3934

    PubMed  CAS  Google Scholar 

  • Saito N, Kose A, Ito A, Hosoda K, Mori M, Hirata M, Ogita K, Kikkawa U, Ono Y, Igarashi K, Nishizuka Y (1989) Immunocytochemical localization of βII subspecies of protein kinase C in rat brain. Proc Natl Acad Sci USA 86:3409–3413

    PubMed  CAS  Google Scholar 

  • Salamero J, Sztul ES, Howell KE (1990) Exocytic transport vesicles generated in vitro from the trans-Golgi network carry secretory and plasma membrane proteins. Proc Natl Acad Sci USA 87:7717–7721

    PubMed  CAS  Google Scholar 

  • Salminen A, Novick PJ (1987) A ras-like protein is required for a post-Golgi event in yeast secretion. Cell 47:527–538

    Google Scholar 

  • Schmitt HD, Puzicha M, Gallwitz D (1988) Study of a temperature-sensitive mutant of the ras-related YPT1 gene product in yeast suggests a role in the regulation of intracellular calcium. Cell 53:635–647

    PubMed  CAS  Google Scholar 

  • Segev N, Mulholland J, Botstein D (1988) The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery. Cell 52:915–924

    PubMed  CAS  Google Scholar 

  • Serafini T, Orci L, Amherdt M, Brunner M, Kahn RA, Rothman JE (1991) ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein. Cell 67:239–253

    PubMed  CAS  Google Scholar 

  • Söllner T, Whiteheart SW, Brunner M, Erdjument-Bromage H, Geromanos S, Tempst P, Rothman JE (1993) SNAP receptors implicated in vesicle targeting and fusion, Nature 362:318–323

    PubMed  Google Scholar 

  • Sontag J-M, Thierse D, Rouot B, Aunis D, Bader M-F (1991) A pertussis-toxin-sensitive protein controls exocytosis in chromaffin cells at a step distal to the generation of second messengers. Lack of correlation between insulin secretion and cyclic AMP levels. J Biol Chem 263:8615–8620

    Google Scholar 

  • Stow JL, de Almeida B, Narula N, Holtzman EJ, Ercolani L, Ausiello DA (1991) A heterotrimeric G protein Gαi-3, on Golgi membranes regulates the secretion of a heparan sulfate proteoglycan in LLC-PK1, epithelial cells. J Cell Biol 114:1113–1124

    PubMed  CAS  Google Scholar 

  • Tartakoff A, Vassalli P, Detraz M (1978) Comparative studies of intracellular transport of secretory proteins. J Cell Biol 79:694–707

    PubMed  CAS  Google Scholar 

  • Taylor TC, Melançon P (1991) ADP-ribosylation factor (ARF) mediates the effect of GTPγS on a cell-free intra-Golgi transport assay. J Cell Biol 115:245a

    Google Scholar 

  • Tooze SA, Huttner WB (1990) Cell-free protein sorting to the regulated and constitutive secretory pathways. Cell 60:837–847

    PubMed  CAS  Google Scholar 

  • Tooze SA, Weiss U, Huttner WB (1990) Requirement for GTP hydrolysis in the formation of secretory vesicles. Nature 347:207–208

    PubMed  CAS  Google Scholar 

  • Tougard C, Louvard D, Picart R, Tixier-Vidal A (1983) The rough endoplasmic reticulum and the Golgi apparatus visualized using specific antibodies in normal and tumoral prolactin cells in culture. J Cell Biol 96:1197–1207

    PubMed  CAS  Google Scholar 

  • Toutant M, Aunis D, Bockaert J, Homburger V, Rouot B (1987) Presence of three pertussis toxin substrates and Goα immunoreactivity in both plasma and granule membranes of chromaffin cells. FEBS Lett 215:339–344

    PubMed  CAS  Google Scholar 

  • Tuomikoski T, Felix MA, Doree M, Gruenberg J (1989) Inhibition of endocytic vesicle fusion in vitro by the cell cycle control protein kinase cdc 2. Nature 342:942–945

    PubMed  CAS  Google Scholar 

  • Ullrich S, Wollheim CB (1988) GTP-dependent inhibition of insulin secretion by epinephrine in permeabilized RINm5F cells. Lack of correlation between insulin secretion and cyclic AMP levels. J Biol Chem 263:8615–8620

    PubMed  CAS  Google Scholar 

  • Ullrich S, Wollheim CB (1989) Galanin inhibits insulin secretion by direct interference with exocytosis. FEBS Lett 247:401–404

    PubMed  CAS  Google Scholar 

  • Ullrich S, Prentki MB, Wollheim CB (1990) Somatostatin inhibition of Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells. Biochem J 270:273–276

    PubMed  CAS  Google Scholar 

  • van der Bliek AM, Meyerowitz EM (1991) Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular traffic. Nature 351:411–414

    PubMed  Google Scholar 

  • van der Merwe PA, Millar RP, Wakefield IK, Davidson JS (1991) Inhibition of luteinizing-hormone exocytosis by guanosine 5′-[γ-thio]triphosphate reveals involvement of a GTP-binding protein distal to second-messenger generation. Biochem J 275:399–405

    PubMed  Google Scholar 

  • van der Meulen J, Bhullar RP, Chancellor-Maddison K-A (1991) Association of a 24-kDA GTP-binding protein, Gn24, with human platelet α-granule membranes. FEBS Lett 291:122–126

    PubMed  Google Scholar 

  • Warren G (1985) Membrane traffic and organelle division. Trends Biochem Sci 10:439–443

    CAS  Google Scholar 

  • Waters MG, Serafini T, Rothman JE (1991) “Coatomer”: a cytosolic protein complex containing subunits of non-clathrin coated Golgi transport vesicles. Nature 349:248–251

    PubMed  CAS  Google Scholar 

  • Wessling-Resnick M, Braell W (1990) Characterization of the mechanism of endocytic vesicle fusion in vitro. J Biol Chem 265:16751–16759

    PubMed  CAS  Google Scholar 

  • Wheeler-Jones CPD, Saermark T, Kakkar W, Authi KS (1992) Mastoparan promotes exocytosis and increases intracellular cyclic AMP in human platelets. Evidence for the existence of a Ge-like mechanism of secretion. Biochem J 281:465–472

    PubMed  CAS  Google Scholar 

  • Woodman PG, Mundy DI, Cohen P, Warren G (1992) Cell-free fusion of endocytic vesicles is regulated by phosphorylation. J Cell Biol 116:331–338

    PubMed  CAS  Google Scholar 

  • Yokokawa N, Komatsu M, Takeda T, Aizawa T, Yamada T (1989) Mastoparan, a wasp venom, stimulates insulin release by pancreatic islets through pertussis toxin sensitive GTP-binding protein. Biochem Biophys Res Commun 158:712–716

    PubMed  CAS  Google Scholar 

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  1. A. Luini
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  2. M. A. De Matteis
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Editors and Affiliations

  1. Department of Medicine, Cell Biology and Experimental Therapeutics, Room 3C-385, Houston VA Medical Center, Baylor College of Medicine, 2002 Holcombe Boulevard, Houston, TX, 77030, USA

    Burton F. Dickey M.D.

  2. Department of Cell Biology, Molecular Physiology and Biophysics, Medicine and Neurosciences, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA

    Lutz Birnbaumer Ph.D.

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Luini, A., De Matteis, M.A. (1993). Regulated Exocytosis and Interorganelle Vesicular Traffic: A Comparative Analysis. In: Dickey, B.F., Birnbaumer, L. (eds) GTPases in Biology I. Handbook of Experimental Pharmacology, vol 108 / 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78267-1_32

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