Skip to main content
SpringerLink
Log in

Endocytic Function in Cell-Free Systems

  • Chapter
GTPases in Biology I

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 108 / 1))

  • 168 Accesses

Abstract

Endocytosis is a dynamic cellular process through which the vesicle-mediated uptake of macromolecules can occur. The internalization of extracellular material proceeds via two related pathways: pinocytosis of macromolecules in the bulk fluid phase and receptor-mediated endocytosis of ligand bound to specific cell surface receptors which are internalized by endocytic vesicles. Functionally, endocytosis enables the cell to harvest key nutrient factors, including iron from transferrin, cholesterol from low-density lipoprotein (LDL), and vitamin B12. Furthermore, receptor-mediated endocytosis plays a vital role in the downregulation of receptors in response to target hormones. In fact, characterization of the major cellular events in receptor-mediated endocytosis has been afforded by examining the internalization of ligands recognized by receptors (for a recent review, see Smythe and Warren 1991).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Balch WE (1989) Biochemistry of interorganelle transport. J Biol Chem 264:16965–16968

    PubMed  CAS  Google Scholar 

  • Bailly E, McCaffrey M, Touchot N, Zahraoui A, Goud B, Bornens M (1991) Phosphorylation of two small GTP-binding proteins of the rab family be p34cdc2. Nature 350:715–718

    Article  PubMed  CAS  Google Scholar 

  • Bomsel M, Parton R, Kuznetsov SA, Schroer TA, Gruenberg J (1990) Microtubule-and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells. Cell 62:719–731

    Article  PubMed  CAS  Google Scholar 

  • Braell WA (1987) Fusion between endocytic vesicles in a cell-free system. Proc Natl Acad Sci USA 84:1137–1141

    Article  PubMed  CAS  Google Scholar 

  • Chavrier P, Gorvel JP, Stelzer E, Simons K, Gruenberg J, Zerial M (1991) Hypervariable C-terminal domain of rab proteins acts as a targeting signal. Nature 353:769–772

    Article  PubMed  CAS  Google Scholar 

  • Chavrier P, Parton RG, Hauri HP, Simons K, Zerial M (1990) Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments. Cell 62:317–329

    Article  PubMed  CAS  Google Scholar 

  • Colombo MI, Gonzalo S, Weidman P, Stahl P (1991) Characterization of trypsin-sensitive factors required for endosome-endosome fusion. J Biol Chem 266:23438–23445

    PubMed  CAS  Google Scholar 

  • Colombo MI, Mayorga LS, Casey PJ, Stahl PD (1992) Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion. Science 255:1695–1697

    Article  PubMed  CAS  Google Scholar 

  • Davey J, Hurtley SI, Warren G (1985) Reconstitution of an endocytic fusion event in a cell-free system. Cell 43:643–652

    Article  PubMed  CAS  Google Scholar 

  • Diaz R, Mayorga L, Stahl P (1988) In vitro fusion of endosomes following receptor-mediated endocytosis. J Biol Chem 263:6093–6100

    PubMed  CAS  Google Scholar 

  • Diaz R, Mayorga L, Weidman PJ, Rothman J, Stahl P (1989) Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport. Nature 339:398–400

    Article  PubMed  CAS  Google Scholar 

  • Goda Y, Pfeffer SR (1988) Selective recycling of the mannose-6-phosphate/IGF-II receptor to the trans-Golgi network in vitro. Cell 55:309–320

    Article  PubMed  CAS  Google Scholar 

  • Gorvel JP, Chavrier P, Zerial M, Gruenberg J (1991) Rab5 controls early endosome fusion in vitro. Cell 64:915–925

    Article  PubMed  CAS  Google Scholar 

  • Griffiths G, Gruenberg J (1991) The arguments for pre-existing early and late endosomes. Trends Cell Biol 1:5–9

    Article  PubMed  CAS  Google Scholar 

  • Gruenberg J, Howell KE (1986) Reconstitution of vesicle fusions occurring in endocytosis with a cell-free system. EMBO J 5:3091–3101

    PubMed  CAS  Google Scholar 

  • Gruenberg J, Griffiths G, Howell KE (1989) Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in vitro. J Cell Biol 108:1301–1316

    Article  PubMed  CAS  Google Scholar 

  • Higashijima T, Graziano MP, Suga H, Kainosho M, Gilman AG (1991) 19F and 31P NMR spectroscopy of G protein α subunits. J Biol Chem 266:3396–3401

    PubMed  CAS  Google Scholar 

  • Joyce-Brady M, Rubins JB, Panchenko MP, Bernardo J, Steele MP, Kolm L, Simons ER, Dickey BF (1991) Mechanisms of mastoparan-stimulated surfactant secretion from isolated pulmonary alveolar type 2 cells. J Biol Chem 266:6856–6859

    Google Scholar 

  • Kahn RA (1991) Fluoride is not an activator of the smaller (20–25 kDa) GTP-binding proteins. J Biol Chem 266:15595–15597

    PubMed  CAS  Google Scholar 

  • Kessel I, Holst BD, Roth TF (1989) Membranous intermediates in endocytosis are labile, as shown in a temperature-sensitive mutant. Proc Natl Acad Sci USA 4968-4972

    Google Scholar 

  • Kinsella BT, Maltese WA (1991) Rab GTP-binding proteins implicated in vesicular transport are isoprenylated in vitro at cysteines within a novel carboxyl-terminal motif. J Biol Chem 266:8540–8544

    PubMed  CAS  Google Scholar 

  • Kreiger M, Kingsley D, Sege R, Hobbie L, Kozarsky K (1985) Genetic analysis of receptor-mediated endocytosis. Trends Biochem Sci 10:447–452

    Article  Google Scholar 

  • Lenhard JM, Kahn RA, Stahl PD (1992) Evidence for ADP-ribosylation factor (ARF) as a regulator of in vitro endosome-endosome fusion. J Biol Chem 267:13047–13052

    PubMed  CAS  Google Scholar 

  • Lin HC, Moore MS, Sanan DA, Anderson RGW (1991) Reconstitution of clathrin-coated pit budding from plasma membranes. J Cell Biol 114:881–891

    Article  PubMed  CAS  Google Scholar 

  • Mahaffey DT, Moore MS, Brodsky FM, Anderson RGW (1989) Coat proteins isolated from clathrin coated vesicles can assemble into coated pits. J Cell Biol 108:1615–1624

    Article  PubMed  CAS  Google Scholar 

  • Mayorga LS, Diaz R, Stahl PD (1989a) Regulatory role for GTP-binding proteins in endocytosis. Science 244:1475–1477

    Article  PubMed  CAS  Google Scholar 

  • Mayorga LS, Diaz R, Colombo MI, Stahl PD (1989b) GTPγS stimulation of endosome fusion suggests a role for a GTP-binding protein in the priming of vesicles before fusion. Cell Regul 1:113–124

    PubMed  CAS  Google Scholar 

  • Moore MS, Mahaffey DT, Brodsky FM, Anderson RGW (1987) Assembly of clathrin-coated pits onto purified plasma membranes. Science 236:558–563

    Article  PubMed  CAS  Google Scholar 

  • Mullock BM, Branch WJ, van Schaik M, Gilbert LK, Luzio JP (1989) Reconstitution of an endosome-lysosome interaction in a cell-free system. J Cell Biol 108:2093–2099

    Article  PubMed  CAS  Google Scholar 

  • Murphy RF (1991) Maturation models for endosome and lysosome biogenesis. Trends Cell Biol 1:77–82

    Article  PubMed  CAS  Google Scholar 

  • Podbilewicz B, Mellman I (1990) ATP and cytosol requirements for transferrin recycling in intact and disrupted MDCK cells. EMBO J 9:3477–3487

    PubMed  CAS  Google Scholar 

  • Riezman H, Chvatchko Y, Dulic V (1986) Endocytosis in yeast. Trends Biochem Sci 11:325–328

    Article  CAS  Google Scholar 

  • Roederer M, Barry JR, Wilson RB, Murphy RF (1990) Endosomes can undergo an ATP-dependent density increase in the absence of dense lysosomes. Eur J Cell Biol 51:229–234

    PubMed  CAS  Google Scholar 

  • Schmid SL, Smythe E (1991) Stage-specific assays for coated pit formation and coated vesicle budding in vitro. J Cell Biol 114:869–880

    Article  PubMed  CAS  Google Scholar 

  • Shpetner HS, Vallee RB (1992) Dynamin is a GTPase stimulated to high levels of activity by microtubules. Nature 355:733–735

    Article  PubMed  CAS  Google Scholar 

  • Simons K, Virta H (1987) Perforated MDCK cells support intracellular transport. EMBO J 6:2241–2247

    PubMed  CAS  Google Scholar 

  • Smythe E, Warren G (1991) The mechanisms of receptor-mediated endocytosis. Eur J Biochem 202:689–699

    Article  PubMed  CAS  Google Scholar 

  • Smythe E, Pypaert M, Lucocq J, Warren G (1989) Formation of coated vesicles from coated pits in broken A431 cells. J Cell Biol 108:843–853

    Article  PubMed  CAS  Google Scholar 

  • Tuomikoski T, Felix MA, Doree M, Gruenberg J (1989) Inhibition of endocytic vesicle fusion in vitro by the cell-cycle control protein kinase cdc2. Nature 342:942–945

    Article  PubMed  CAS  Google Scholar 

  • Van der Sluijs P, Hull M, Zahraoui A, Tavitian A, Goud B, Mellman I (1991) The small GTP-binding protein rab4 is associated with early endosomes. Proc Natl Acad Sci USA 88:6313–6317

    Article  Google Scholar 

  • Warren G (1989) Mitosis and membranes. Nature 342:857–858

    Article  PubMed  CAS  Google Scholar 

  • Wessling-Resnick M, Braell WA (1990a) The sorting and segregation mechanism of the endocytic pathway is functional in a cell-free system. J Biol Chem 265:690–699

    PubMed  CAS  Google Scholar 

  • Wessling-Resnick M, Braell WA (1990b) Characterization of the mechanism of endocytic vesicle fusion in vitro. J Biol Chem 265:16751–16759

    PubMed  CAS  Google Scholar 

  • Woodman PG, Warren G (1988) Fusion between vesicles from the pathway of receptor-mediated endocytosis in a cell-free system. Eur J Biochem 173:101–108

    Article  PubMed  CAS  Google Scholar 

  • Woodman PG, Warren G (1991) Isolation of functional, coated, endocytic vesicles. J Cell Biol 112:1133–1141

    Article  PubMed  CAS  Google Scholar 

  • Zahraoui A, Touchot N, Chardin P, Tavitian A (1989) The Human Rab genes encode a family of GTP-binding proteins related to yeast Ypt1 and Sec4 products involved in secretion. J Biol Chem 264:12394–12401

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. M. Wessling-Resnick
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Medicine, Cell Biology and Experimental Therapeutics, Room 3C-385, Houston VA Medical Center, Baylor College of Medicine, 2002 Holcombe Boulevard, Houston, TX, 77030, USA

    Burton F. Dickey M.D.

  2. Department of Cell Biology, Molecular Physiology and Biophysics, Medicine and Neurosciences, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA

    Lutz Birnbaumer Ph.D.

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Wessling-Resnick, M. (1993). Endocytic Function in Cell-Free Systems. In: Dickey, B.F., Birnbaumer, L. (eds) GTPases in Biology I. Handbook of Experimental Pharmacology, vol 108 / 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78267-1_30

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-78267-1_30

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78269-5

  • Online ISBN: 978-3-642-78267-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation