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Transporting Cl−-ATPase in Rat Brain

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Electrogenic Cl− Transporters in Biological Membranes

Part of the book series: Advances in Comparative and Environmental Physiology ((COMPARATIVE,volume 19))

Abstract

Intracellular Cl− concentration ([Cl−]i) is an essential factor in the regulation of neuronal excitability through receptor-operated Cl− channels (Alger 1985; Barker 1985). Evidence for chloride activities higher or lower than those expected from a passive distribution (Alvarez-Leefmans 1990) has suggested the presence of inwardly or outwardly directed active Cl− transport mechanisms. In neurons, uphill accumulation of Cl− is known to be mediated by Na+/K+/Cl− co-transporters in both invertebrate and vertebrate neurons (Russell 1983; Ballanyi and Grafe 1985; Alvarez-Leefmans et al. 1988). However, the mechanisms underlying the outwardly directed Cl− transport have not been fully elucidated. Thompson et al. (1988a, b) and Thompson and Gahwiler (1989a, b) have suggested that active Cl− extrusion from mammalian cortical neurons is mediated by outwardly directed Cl−/cation co-transport mechanism. On the other hand, neuronal Cl−-stimulated ATPase acting as an ATP-driven Cl− pump has also been a candidate for such an outwardly directed Cl− transport system (Inagaki et al. 1985, 1987; Inagaki and Shiroya 1988; Shiroya et al. 1989b; Inoue et al. 1991a; Hara et al. 1992). We report in this chapter on the Cl− stimulated ATPase which outwardly transports Cl− across neuronal plasma membranes.

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Authors and Affiliations

  1. Department of Pharmacology, Kansai Medical University, Osaka 570, Japan

    C. Inagaki, M. Hara & M. Inoue

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  1. C. Inagaki
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  2. M. Hara
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  3. M. Inoue
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Editors and Affiliations

  1. Department of Physiology College of Medicine, University of Florida, Box 100274, 32610, Gainesville, FL, USA

    George A. Gerencser

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Inagaki, C., Hara, M., Inoue, M. (1994). Transporting Cl−-ATPase in Rat Brain. In: Gerencser, G.A. (eds) Electrogenic Cl− Transporters in Biological Membranes. Advances in Comparative and Environmental Physiology, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78261-9_4

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  • DOI: https://doi.org/10.1007/978-3-642-78261-9_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78263-3

  • Online ISBN: 978-3-642-78261-9

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