The Phosphorylated Ribosomal Protein in Tetrahymena is Homologous with Mammalian Ribosomal Protein S4

  • Lisbeth Palm
  • Peter Højrup
  • Karsten Kristiansen
Conference paper
Part of the NATO ASI Series book series (volume 76)


Phosphorylation of a restricted number of ribosomal proteins has been described for all eukaryotic cells analyzed to date. The actual number of phosphorylated ribosomal proteins varies, but a single basic ribosomal protein with a Mr around 30,000 is the most prominent phosphoprotein in eukaryotic ribosomes (Wool, 1979; Cuny, 1985; Brathohn, 1986 ). In mammals up to five phosphate groups can be incorporated into this protein, designated S6 (Thomas et al., 1980), and increased S6 phosphorylation is an apparent prerequisite for activation of protein synthesis in response to growth factors (Thomas et al., 1982).


Ribosomal Protein Cyanogen Bromide Lower Eukaryote Eukaryotic Ribosome Plasma Desorption Mass Spectrometry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bommer U-A, Noll F, Lutsch G, Bielka H (1980) Immunochemical detection of proteins in the small subunit of rat liver ribosomes involved in binding of the ternary initiation complex. FEBS Lett 111:171–174.PubMedCrossRefGoogle Scholar
  2. Bratholm PS (1986) Phosphorylation of ribosomal protein S7 in Tetrahymena. Thesis, University of Odense.Google Scholar
  3. Cuny M (1985) Contribution a l’etude du ribosome de Tetrahymena. Thesis, University of Paris VII.Google Scholar
  4. Cuny M, Sripati CE, Hayes DH (1985) The specific phosphorylation of a 40S ribosomal protein in growth-arrested Tetrahymena is induced by sodium. J Cell Phys 124:349–357.CrossRefGoogle Scholar
  5. Hansen TS, Andreasen PH, Dreisig H, Højrup P, Nielsen H, Engberg J, Kristiansen K (1991) Tetrahymena thermophila acidic ribosomal protein L37 contains an archaebacterial type of C-terminus. Gene 105:143–150.PubMedCrossRefGoogle Scholar
  6. Johnson SP, Warner JR (1987) Phosphorylation of Saccharomyces cerevisiae equivalent of ribosomal protein S6 has no detectable effect on growth. Mol Cell Biol 7:1338–1345.PubMedGoogle Scholar
  7. Krieg J, Hofsteenge J, Thomas G (1988) Identification of the 40S ribosomal protein S6 phosphorylation sites induced by cycloheximide. J Biol Chem 263:11473–11477.PubMedGoogle Scholar
  8. Kristiansen K, Plesner P, Krüger A (1978) Phosphorylation in vivo of ribosomes in Tetrahymena pyriformis. Eur J Biochem 83:395–403.PubMedCrossRefGoogle Scholar
  9. Leer RJ, van Raamsdonk-Duin MMC, Molenaar CMT, Cohen LH, Mager WH, Planta RJ (1982) The structure of the gene coding for the phosphorylated ribosomal protein S10 in yeast. Nucl Acids Res 10:5869–5878.PubMedCrossRefGoogle Scholar
  10. Marvaldi J, Lucas-Lenard J (1977) Differences in the ribosomal protein gel profile after infection of L cells with wild type or temperature-sensitive mutants of vesicular stomatitis virus. Biochemistry 16:4320–4327.PubMedCrossRefGoogle Scholar
  11. Nygård O, Nika H (1982) Identification by RNA-protein cross-linking of ribosomal proteins located at the interface between the small and the large subunits of mammalian ribosomes. EMBO J 1:357–362.PubMedGoogle Scholar
  12. Terao K, Ogata K (1979) Proteins of small subunits of rat liver ribosomes that interact with poly(U). II. Crosslinks between poly(U) and ribosomal proteins in 40S subunits induced by UV irradiation. J Biochem (Tokyo) 86:605–617.Google Scholar
  13. Thomas G, Siegmann M, Kubler A-M, Gordon J, Jimenez de Asua L (1980) Regulation of 40S ribosomal protein S6 phosphorylation in swiss mouse 3T3 cells. Cell 19:1015–1023.PubMedCrossRefGoogle Scholar
  14. Thomas G, Martin-Pérez J, Siegmann M, Otto AM (1982) The effect of serum, EGF, PGF and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis. Cell 30:235–242.PubMedCrossRefGoogle Scholar
  15. Uchiumi T, Kikuchi M, Ogata K (1986) Cross-linking study on protein neighborhoods at the subunit interface of rat liver ribosomes with 2-iminothiolane. J Biol Chem 261:9663–9667.PubMedGoogle Scholar
  16. Wool IG (1979) The structure and function of eukaryotie ribosomes. Ann Rev Biochem 48:719–754.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Lisbeth Palm
    • 1
  • Peter Højrup
    • 1
  • Karsten Kristiansen
    • 1
  1. 1.Department of Molecular BiologyUniversity of OdenseOdense MDenmark

Personalised recommendations