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Growth Hormone (GH) Increases Lipoprotein Lipase mRNA in Cells Transfected with the Rat GH Receptor cDNA

  • S. M. Francis
  • S. Enerbäck
  • C. Möller
  • B. Enberg
  • G. Norstedt
Conference paper
Part of the NATO ASI Series book series (volume 76)

Abstract

The growth hormone (GH) receptor is phosphorylated on tyrosine residues after ligand binding (Foster et al. 1988). However the mechanism of intracellular signalling is unclear, since the receptor shows no apparent homology to classical tyrosine kinase receptors (Leung et al. 1987). One obstacle to in vitro studies is that few cultured cells express high levels of the GH receptor. The approach taken here was to transfect cultured cells with expression plasmids containing the rat GH receptor cDNA and the gene coding for neomycin resistance. Drug resistant cell colonies expressing high levels of GH receptors, both at the mRNA and protein level, were selected. Several different cell lines were stably transfected in this manner : Chinese hamster ovary cells showed GH-induced protein synthesis as well as GH-induced phosphorylation of MAP kinases (Emtner et al. 1990; Möller et al. 1992). The transfected Buffalo rat liver (BRL) cells in this study showed a GH-induced increase in lipoprotein lipase (LPL) mRNA indicating that the expressed receptor is functional.

Keywords

Growth Hormone Chinese Hamster Ovary Cell Lipoprotein Lipase Growth Hormone Receptor Lipoprotein Lipase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Auwerx JH, Deeb S, Brunzell JD, Wolfbauer G, Chait A (1989) Lipoprotein lipase gene expression in THP-1 cells. Biochemistry 28: 4563–4567.PubMedCrossRefGoogle Scholar
  2. Emtner M, Mathews LS, Norstedt G (1990) Growth Hormone (GH) Stimulates Protein Synthesis in Cells Transfected with GH Receptor Complementary DNA. Mol Endocrinol 4: 2014–2020.PubMedCrossRefGoogle Scholar
  3. Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M (1987) Lipofection: A highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci USA 84:7413–7417.PubMedCrossRefGoogle Scholar
  4. Fort P, Marty L, Piechaczyk M, Sabrouty SE, Dani C, Jeanteur P, Blanchard JM (1985) Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. Nucleic Acids Res 13: 1431–1442.PubMedCrossRefGoogle Scholar
  5. Foster CM, Shafer JA, Rozsa FW, Wang X, Lewis SD, Renken DA, Natale JE, Schwartz J, Carter-Su C (1988) Growth Hormone Promoted Tyrosyl Phosphorylation of Growth Hormone Receptors in Murine 3T3-F442A Fibroblasts and Adipocytes. Biochemistry 27: 326–334.PubMedCrossRefGoogle Scholar
  6. Kirchgessner TG, Svenson KL, Lusis AJ, Schotz MC (1987) The sequence of cDNA Encoding Lipoprotein Lipase. A member of a lipase gene family. J Biol Chem 262: 8463–8466.PubMedGoogle Scholar
  7. Leung DW, Spencer SA, Cachianes G, Hammonds RG, Collins C, Henzel WJ, Barnard R, Waters MJ, Wood WI (1987) Growth hormone receptor and serum binding protein: purification, cloning and expression. Nature 330: 537–543.PubMedCrossRefGoogle Scholar
  8. Mathews LS, Enberg B, Norstedt G (1989) Regulation of Rat Growth Hormone Receptor Gene Expression. J Biol Chem 264:9905–9910.PubMedGoogle Scholar
  9. Möller C, Arner P, Sonnenfeld T, Norstedt G (1991) Quantitative Comparison of Insulin-Like Growth Factor Messenger RNA Levels in Human and Rat Tissues Analysed by a Solution Hybridization Assay. J Mol Endocrinol 7:213–222.PubMedCrossRefGoogle Scholar
  10. Möller C, Hansson A, Enberg B, Lobie PE, Norstedt G (1992) Growth Hormone induction of tyrosine phosphorylation and activation of mitogen activated protein kinases in cells transfected with rat GH receptor cDNA. J Biol Chem In Press.Google Scholar
  11. Pradines FA, Barcellini CS, Dani C, Vannier C, Ailhaud G (1990) Transcriptional control of the expression of lipoprotein lipase gene by growth hormone in preadipocyte Ob1771 cells. J Lipid Res 31: 1283–1291.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • S. M. Francis
    • 1
  • S. Enerbäck
    • 1
    • 2
  • C. Möller
    • 1
  • B. Enberg
    • 1
  • G. Norstedt
    • 1
  1. 1.Center for BiotechnologyKarolinska InstituteHuddingeSweden
  2. 2.Department of Molecular BiologyUniversity of GöteborgGöteborgSweden

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