Localization-Dependent Regulation of Lymphocyte Tyrosine Phosphatases

  • Christine Stader
  • Hans Werner Hofer
Conference paper
Part of the NATO ASI Series book series (volume 76)

Abstract

A soluble tyrosine phosphatase isolated from pig spleen was strongly inhibited by nucleic acid and anionic phospholipids (1,2). Potentially, therefore, the enzyme could be regulated by reversible binding to membranes or nucleic acid — containing structures. In order to examine this hypothesis we studied the localization of tyrosine phosphatases in lymphoid cells.

Keywords

Filtration EDTA Tyrosine Heparin Vanadate 

Abbreviations

CHAPS

3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-pro-panolsulfonat

HMC

high molecular weight complex

MBP

myelin basic protein

PTP

phosphotyrosine protein phosphatase

RCML

reduced carboxymethylated and maleylated lysozyme

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Stader, C., Dierig, S., Tidow, N., Kirsch, S., Hofer, H.W. (1991) in: Cellular Regulation by Protein Phosphorylation (Heilmeyer, L.M.G. ed.) pp. 355–360.Google Scholar
  2. 2.
    Stader, C., Dierig, S., Hofer, H. W. (1991) Adv. Protein Phosphatases 6 (Suppl.), 89–90.Google Scholar
  3. 3.
    Cool, D.E., Tonks, N.K., Charbonneau, H., Walsh, K.A., Fischer, E.H., Krebs, E.G. (1989)Google Scholar
  4. 4.
    Cool, D.E., Tonks, N.K., Charbonneau, H., Fischer, E.H., Krebs, E.G. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 7280–7284.PubMedCrossRefGoogle Scholar
  5. 5.
    Daum, G., Zander, N.F., Morse, B., Hurwitz, D., Schlessinger, J., Fischer, E.H. (1991) J. Biol. Chem. 266, 12211–12215.PubMedGoogle Scholar
  6. 6.
    Boylan, J.M., Brautigan, D.L., Madden, J., Raven, T., Ellis, L, Gruppuso, P.A. (1992) J. Clin. Invest. 90, 174–179.PubMedCrossRefGoogle Scholar
  7. 7.
    Zander, N.F., Lorenzen, J.A., Cool D.E., Tonks, N.K., Daum, G., Krebs, E.G., Fischer, E.H. (1991) Biochemistry 30, 6964–6970.PubMedCrossRefGoogle Scholar
  8. 8.
    Brautigan, D.L., Pinault, F.M. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 6696–6700.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Christine Stader
    • 1
  • Hans Werner Hofer
    • 1
  1. 1.Faculty of BiologyUniversity of KonstanzKonstanzGermany

Personalised recommendations