Abstract
Polymerase chain reaction (PCR) is a sensitive molecular method that can detect very small amounts of DNA. DNA is a highly stable molecule that can survive in the environment even when the host organism is no longer viable. PCR therefore promises to be a powerful tool for diagnosis of H. pylori infection.
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References
Czinn SJ, Carr HS, Speck WT (1991) Diagnosis of gastritis caused by Heilcobactor pylori in children by means of an ELISA. Rev Infect Dis 13 [Suppl 8]: S700-S703
Westblom TU, Madan E, Gudipati S, Midkiff BR, Czinn SJ (1992) Diagnosis of Heilcobactor pylori infection in adult and pediatric patients using pyloriset, a rapid latex agglutination test. J Clin Microbiol 30:96–98
Marshall BJ, Surveyor I (1988) Carbon-14 urea breath test for the diagnosis of Campylobacter pylori associated gastritis. J Nucl Med 29:11–16
Westblom TU (1991) Laboratory diagnosis and handling of Helicobacter pylori. In: Marshall BJ, McCallum RW, Guerrant RL (eds) Helicobacter pylori in peptic ulceration and gastritis. Black well Scientific, Cambridge, pp 81–91
Delmee M, Debongnie JC, Warzee P, Mainguet P (1987) Une method originale de prelevement bactériologique pour l’etude de Campylobacter pylori dans l’ulcere duodenal. Gastroenterol Clin Biol 11:550–553
Debongnie JC, Delmee M, Mainguet P (1989) Campylobacter pylori in gastric, duodenal and jejunal juices and mucosae of patients with duodenal ulcer. Acta Gastroenterol Belg 52: 3–8
Bazhenov LG, Saidkhanov BA, Khodzhaeva NU (1990) The determination of C. pylori in patients with gastroduodenal diseases. Lab Delo (10): 71–73
Westblom TU, Phadnis S, Normark S (1991) Polymerase chain reaction (PCR) for diagnosis of Helicobacter pylori infection. Gastroenterology 100:A625
Westblom TU, Phadnis S, Normark S (1991) Sensitivity of an assay for detection of Helicobacter pylori in human saliva using polymerase chain reaction (PCR). Microbiol Ecol Hlth Dis 4(S):S156
Yap EPH, McGee JOD (1991) Short PCR product yields improved by lower denaturation temperatures. Nucleic Acids Res 19:1713
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© 1994 Springer-Verlag Berlin Heidelberg
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Westblom, T.U., Phadnis, S., Normark, S., Czinn, S.J. (1994). Diagnosis of Helicobacter pylori in Gastric Juice Aspirates Using Polymerase Chain Reaction. In: Gasbarrini, G., Pretolani, S. (eds) Basic and Clinical Aspects of Helicobacter pylori Infection. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78231-2_38
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DOI: https://doi.org/10.1007/978-3-642-78231-2_38
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