The Value of Class and Subclass ELISAs and Antibody Specificity in Monitoring Treatment of Helicobacter pylori

  • A. R. Stacey
  • G. D. Bell
  • D. G. Newell
Conference paper

Abstract

Serology has been proposed as a non-invasive test for the monitoring of treatment to eradicate Helicobacter pylori infection. However, the total IgG response associated with this chronic mucosal infection is generally long lived. Preliminary data [3] suggested that certain IgG subclasses had I shorter life and were better indicators of successful treatment. Moreover, some antigens would be expected preferentially to induce antibody responses with shorter half-hves. Therefore the persistence of patient serum anti-H. pylori class and subclass responses was investigated in sequential samples from both successfully and unsuccessfully treated patients followed over a 12-month period. In addition, the antigen specificity of these antibodies was studied using wastern blotting techniques.

Keywords

Urea Polypeptide Catalase Gastritis Sera 

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References

  1. 1.
    Bell GD, Weil J, Harrison G, Morden A, Jones PH, Gant PW, Trowell JE, Yoong AK, Daneshemnd TK, Logan RFA (1987) 14C-Urea breath test analysis, a non-invasive test for Campylobacter pylori in the stomach. Lancet 1:1367–1368PubMedCrossRefGoogle Scholar
  2. 2.
    Newell DG (1987) Human serum antibody responses to the surface protein antigens of Campylobacter pyloridis. Serodiag Immunother. 1: 209–217CrossRefGoogle Scholar
  3. 3.
    Newell DG, Bell GD, Weil J, Jones P, Grant P, Harrison G (1989) The effect of treatment on circulating anti-Helicobacter pylori antibodies-a two-year follow-up study. In: Malfertheiner P, Ditschuneit H (eds) Helicobacter pylori, gastritis and peptic ulcer. Springer, Berlin Heidelberg New York, pp 172–175Google Scholar
  4. 4.
    Stacey AR, Hawtin PR, Newell DG (1990) Antigenicity of fractions of Helicobacter pylori prepared by fast liquid protein chromatography and urease captured by monoclonal antibodies. Eur J Clin Microbiol Infect Dis 9:732–737PubMedCrossRefGoogle Scholar
  5. 5.
    Steer HW, Hawtin PR, Newell DG (1987) ELISA for serodiagnosis of Campylobacter pyloridis infection in patients with gastritis and benign peptic ulceration. Serodiag Immunother 1: 253–259CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • A. R. Stacey
    • 1
  • G. D. Bell
    • 2
  • D. G. Newell
    • 3
  1. 1.PHLS Centre for Applied Microbiology and ResearchPorton Down, SalisburyUK
  2. 2.Department of MedicineIpswich HospitalIpswichUK
  3. 3.Central Veterinary LaboratoryWeybridgeUK

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