Abstract
Bacterial surface haemaglutinins and lectins play a significant role in pathogenesis of many mucus-associated infections. A number of reports describe cell surface haemagglutinins of Helicobacter pylori [2,3,8,10,11] that may mediate adhesion to stomach tissues. Robinson et al. [10] recently reported that some strains of H. pylori incubated in phosphate-buffered sahne (PBS) released soluble haemagglutinins (SHAs) active against human and guniea pig erythrocytes that specifically bind to a NeuAc(2–3)Gal structure and also have high affinity for another sialic acid containing structure. Evans et al. [3] has described a fibrillar haemagglutinin of H. pylori which binds to NeuAc(2–3)Gal isomer of NeuAc-lactose. In this study we describe the partial purification and characterization of soluble haemagglutinin(s) of H. pylori strain NCTC 11637.
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References
Bernheimer AW (1988) Assay of hemolytic toxins. Methods Enzymol 165:213–225
Emödy L, Carlsson Å, Ljungh Å, Wadström T (1988) Mannose-resistant haemagglutination by Campylobacter pylori. Scand J Infect Dis 20:353–354
Evans DG, Evans DJ, Moulds JJ, Graham DY (1988) N-Acetylneuraminlactose-binding fibrillar hemagglutinin of Campylobacter pylori: a putative colonization factor antigen. Infect Immun 56:2896–2906
Isemura M, Schmid K (1971) Studies on the carbohydrate moiety of a α1-acid glycoprotein (orosomucoid) by using alkaline hydrolysis and deamination by nitrous acid. Biochem J 124:591–604
Kothary MH, Kreger AS (1985) Production and partial characterization of an elastolytic protease of Vibrio vulnificus. Infect Immun 49:25–31
Kurioka S, Matsuda M (1976) Phospholipase C assay using p-nitrophenylphosphorylcholine together with sorbitol and its application to studying the metal and detergent requirement of the enzyme. Anal Biochem 75:281–289
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Nakazawa T, Ishibashi M, Konishi H, Takemoto T, Shigeeda M, Kochiyama T (1989) Hemagglutination activity of Campylobacter pylori. Infect Immun 57:989–991
Nilsson B, Norden NE, Svensson S (1979) Structural studies on the carbohydrate portion of fetuin. J Biol Chem 254:4545–4553
Robinson J, Goodwin CS, Cooper M, Burke V, Mee BJ (1990) Soluble and cell-associated haemagglutinins of Helicobacter (Campylobacter) pylori. J Med Microbiol 33:277–284
Wadström T, Guruge JL, Wei S, Aleljung P, Ljungh Å (1990) Helicobacter pylori Hemagglutinin — possible gut mucosa adhesion. In: Malferthiener P, Ditschuneit H (eds) Helicobacter pylori, gastritis and peptic ulcer. Springer, Berlin Heidelberg New York, pp 96–103
Wadström T, Trust TJ (1984) Bacterial surface lectins. Med Microbiol 4:287–334
Winter A, Karlsson C (1976) Preparative electrofocusing in density gradients. LKB application note 219. LKB-Produkter AB Bromma, Sweden
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© 1994 Springer-Verlag Berlin Heidelberg
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Lelwala-Guruge, J., Kreger, A.S., Shen, W., Wadström, T. (1994). Partial Purification and Characterization of Sialic Acid Specific Soluble Haemagglutinin(s) of Helicobacter pylori Strain NCTC 11637. In: Gasbarrini, G., Pretolani, S. (eds) Basic and Clinical Aspects of Helicobacter pylori Infection. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78231-2_19
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DOI: https://doi.org/10.1007/978-3-642-78231-2_19
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