Construction of a Molecular Population Genetic Framework for the Characterization of Helicobacter pylori Pathogenesis by Multilocus Enzyme Electrophoresis

  • M. F. Go
  • D. Y. Graham
  • J. M. Musser
Conference paper

Abstract

Helicobacter pylori is now recognized as the most common cause of gastritis. H. pylori has also been implicated in the pathogenesis of duodenal ulcer disease and gastric ulcer disease and may play an important role in the development of precursor lesions of gastric cancer. It remains unclear whether the differences in the presentation of H. pylori associated diseases are due to infection with different genetic strains of H. pylori, variation in the host response, or both. Studies attempting to identify H. pylori strains associated with specific H. pylori diseases have recently begun (e.g., chromosomal and plasmid DNA restriction endonuclease profiling analyses). DNA-DNA solution hybridization of genomic DNA from H. pylori isolates found that H. pylori isolates from patients with duodenal ulcer, on average, formed a different hybridization group from isolates from patients with asymptomatic gastritis [3]. Recent studies of many other pathogenic bacterial species have shown that genetic subpopulations of bacteria are nonrandomly associated with certain clinical disorders [2] and that, even for species that are genetically highly variable, the majority of cases of serious disease are caused by a small proportion of the total bacterial cell lineages.

Keywords

Migration Electrophoresis Polypeptide Nucleoside Gastritis 

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References

  1. 1.
    Selander RK, Caugant DA, Ochman H, Musser JM, Gilmour MN, Whittam TS (1986) Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 51:873–884PubMedGoogle Scholar
  2. 2.
    Selander RK, Musser JM, Caugant DA, Gilmour MN, Whittam TS (1987) Population genetics of pathogenic bacteria. Microb Pathog 3:1–7PubMedCrossRefGoogle Scholar
  3. 3.
    Yoshimura HH, Evans DG, Graham DY (1990)H. pylori strains from duodenal ulcer patients differ at the genomic level from those from patients with simple gastritis. Enferm Dig 78 [Suppl 1]:22Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • M. F. Go
    • 1
  • D. Y. Graham
    • 1
  • J. M. Musser
    • 1
  1. 1.Departments of Medicine and PathologyBaylor College Medicine and VA Medical CenterHoustonUSA

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