Abstract
The most revealing piece of information that can readily be obtained about a peptide is its amino acid composition. Yet, the results of amino acid analysis are really meaningful only if the sample consists of a single peptide. Analysis of mixtures is usually an unrewarding effort. Thus, purification should precede analysis, and this generalization is valid for most other methods of structure determination as well. Homogeneity as a prerequisite of analysis cannot be overemphasized. Purification is sometimes possible by crystallization but in most cases chromatography, electrophoresis or countercurrent distribution or a combination of these techniques is needed.
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Benson, J. R., Louie, P. C., Bradshaw, R. A.: Amino Acid Analysis of Peptides, in The Peptides, Vol. 4 (Gross, E., Meienhofer, J., eds.) pp. 217–260, New York: Academic Press 1981
Brenner, M., Niederwieser, A.: Thin Layer Chromatography of Amino Acids in Methods of Enzymology, Vol. 11 (Hirs, C. H. ed.) pp. 39–59, New York: Academic Press 1967
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© 1993 Springer-Verlag Berlin Heidelberg
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Bodanszky, M. (1993). Amino Acid Analysis. In: Peptide Chemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78206-0_2
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DOI: https://doi.org/10.1007/978-3-642-78206-0_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-56675-5
Online ISBN: 978-3-642-78206-0
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