Solid phase peptide synthesis is the only widely practiced technique of facilitation, but several other methods have also been proposed for the rapid construction of long peptide chains. Some of these alternative approaches have certain advantages over solid phase synthesis: reactions carried out in solution are not affected by the rate-limiting control of the gel. Also, where isolation of intermediates is possible, these can be analyzed and purified. A major disadvantage, however, common to the various techniques discussed below, is that they are less conducive to mechanization and automation than the Merrifield method.
KeywordsActive Ester Solid Phase Peptide Synthesis Activate Amino Acid Acylating Agent Simultaneous Synthesis
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