Quantifying the Smooth Muscle Content of the Prostate Using Double Immunoenzymatic Staining and Color-Assisted Image Analysis

  • Ellen Shapiro
  • Herbert Lepor


Benign prostatic hyperplasia (BPH) describes a hyperplastic process of the stromal and epithelial elements of the prostate (Franks 1975). The clinical manifestations of BPH are related primarily to symptoms of bladder outlet obstruction (Barry 1990). The specific histologic features of the prostate which lead to the development of bladder outlet obstruction are unknown.


Benign Prostatic Hyperplasia Bladder Outlet Obstruction Symptomatic Benign Prostatic Hyperplasia Computer Image Analysis Open Prostatectomy 
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  1. Barry MJ (1990) Epidemiology and natural history of benign prostatic hyperplasia. Urol Clin North Am 17:495PubMedGoogle Scholar
  2. Bartsch G (1977) Stereology, a new quantitative morphological approach to study prostatic function and disease. Eur Urol 3:85–95PubMedGoogle Scholar
  3. Bartsch G, Muller HR, Boerholzer M, Rohr HP (1979) Light microscopic stereologic analysis of the normal human prostate and benign prostatic hyperplasia. J Urol 122:487–491PubMedGoogle Scholar
  4. Boenisch T (1989) Staining methods. In: Naish SJ (ed) Handbook of immunochemical staining methods. DAKO Corporation, Carpintería, Calif., p 13Google Scholar
  5. DeKlerk DP, Coffey DS (1978) Quantitative determination of prostatic epithelial and stromal hyperplasia by a new technique: biomorphometrics. Invest Urol 16:240–245PubMedGoogle Scholar
  6. Franks LM (1975) Benign prostatic hyperplasia: gross and microscopic anatomy. In: Grayhack JT, Wilson JD, Scherbenske MJ (eds) Benign prostatic hyperplasia. National Institutes of Health, Bethesda, Md., p 63Google Scholar
  7. Shapiro E, Hartanto V, Lepor H (1992a) Quantifying the smooth muscle content of the prostate using double-immunoenzymatic staining and color assisted image analysis. J Urol 147: 1167–1170PubMedGoogle Scholar
  8. Shapiro E, Becich MJ, Hartanto V, Lepor H (1992b) The relative proportion of stromal and epithelial hyperplasia is related to the development of symptomatic BPH. J Urol 147:1293–1297PubMedGoogle Scholar
  9. Shapiro E, Hartanto V, Lepor H (1992c) Anti-desmin vs. anti-actin for quantifying the area density of prostate smooth muscle. Prostate 20:259–267PubMedCrossRefGoogle Scholar
  10. Siegel YI, Zaidel L, Hanmel I (1990) Morphometric evaluation of benign prostatic hyperplasia. Eur Urol 18:71–73PubMedGoogle Scholar
  11. Weibel ER, Bolender RP (1973) Stereological techniques for electron microscopic morphometry. In: Hayat MA (ed) Principles and techniques of electron microscopy, vol 3. Van Nostrand Reinhold, New York, pp 237–296Google Scholar
  12. Weibel ER, Kistler GS, Scherle WF (1966) Practical stereological methods for morphometric cytology. J Cell Biol 30:23PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Ellen Shapiro
    • 1
  • Herbert Lepor
    • 1
  1. 1.Department of Urology, School of MedicineNew york UniversityNew YorkUSA

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