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Quantifying the Smooth Muscle Content of the Prostate Using Double Immunoenzymatic Staining and Color-Assisted Image Analysis

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Abstract

Benign prostatic hyperplasia (BPH) describes a hyperplastic process of the stromal and epithelial elements of the prostate (Franks 1975). The clinical manifestations of BPH are related primarily to symptoms of bladder outlet obstruction (Barry 1990). The specific histologic features of the prostate which lead to the development of bladder outlet obstruction are unknown.

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References

  • Barry MJ (1990) Epidemiology and natural history of benign prostatic hyperplasia. Urol Clin North Am 17:495

    PubMed  CAS  Google Scholar 

  • Bartsch G (1977) Stereology, a new quantitative morphological approach to study prostatic function and disease. Eur Urol 3:85–95

    PubMed  CAS  Google Scholar 

  • Bartsch G, Muller HR, Boerholzer M, Rohr HP (1979) Light microscopic stereologic analysis of the normal human prostate and benign prostatic hyperplasia. J Urol 122:487–491

    PubMed  CAS  Google Scholar 

  • Boenisch T (1989) Staining methods. In: Naish SJ (ed) Handbook of immunochemical staining methods. DAKO Corporation, Carpintería, Calif., p 13

    Google Scholar 

  • DeKlerk DP, Coffey DS (1978) Quantitative determination of prostatic epithelial and stromal hyperplasia by a new technique: biomorphometrics. Invest Urol 16:240–245

    PubMed  CAS  Google Scholar 

  • Franks LM (1975) Benign prostatic hyperplasia: gross and microscopic anatomy. In: Grayhack JT, Wilson JD, Scherbenske MJ (eds) Benign prostatic hyperplasia. National Institutes of Health, Bethesda, Md., p 63

    Google Scholar 

  • Shapiro E, Hartanto V, Lepor H (1992a) Quantifying the smooth muscle content of the prostate using double-immunoenzymatic staining and color assisted image analysis. J Urol 147: 1167–1170

    PubMed  CAS  Google Scholar 

  • Shapiro E, Becich MJ, Hartanto V, Lepor H (1992b) The relative proportion of stromal and epithelial hyperplasia is related to the development of symptomatic BPH. J Urol 147:1293–1297

    PubMed  CAS  Google Scholar 

  • Shapiro E, Hartanto V, Lepor H (1992c) Anti-desmin vs. anti-actin for quantifying the area density of prostate smooth muscle. Prostate 20:259–267

    Article  PubMed  CAS  Google Scholar 

  • Siegel YI, Zaidel L, Hanmel I (1990) Morphometric evaluation of benign prostatic hyperplasia. Eur Urol 18:71–73

    PubMed  CAS  Google Scholar 

  • Weibel ER, Bolender RP (1973) Stereological techniques for electron microscopic morphometry. In: Hayat MA (ed) Principles and techniques of electron microscopy, vol 3. Van Nostrand Reinhold, New York, pp 237–296

    Google Scholar 

  • Weibel ER, Kistler GS, Scherle WF (1966) Practical stereological methods for morphometric cytology. J Cell Biol 30:23

    Article  PubMed  CAS  Google Scholar 

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© 1994 Springer-Verlag Berlin Heidelberg

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Shapiro, E., Lepor, H. (1994). Quantifying the Smooth Muscle Content of the Prostate Using Double Immunoenzymatic Staining and Color-Assisted Image Analysis. In: Petrovich, Z., Baert, L. (eds) Benign Prostatic Hyperplasia. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78185-8_5

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  • DOI: https://doi.org/10.1007/978-3-642-78185-8_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78187-2

  • Online ISBN: 978-3-642-78185-8

  • eBook Packages: Springer Book Archive

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