13C and 1H Magnetic Resonance Studies of Normal and Neoplastic Brain Cells

  • D. Leibfritz
  • A. Brand
  • C. Richter-Landsberg
Conference paper


Current volume-selective in vivo magnetic resonance (MR) spectroscopy in humans records signals from volumes larger than 1 cm3. In this case, the spectra contain peaks from various cell types, which is particularly true for organs such as the brain and kidney. Cell cultures are a means of gaining access to the metabolism of individual cells. As MR requires a fairly large number of cells (approximately 108) two strategies may be employed: either cells are grown in several dishes under optimal conditions and then harvested and extracted, or, if enough cells can be supplied adequately in a MR tube, their metabolism is followed in vivo. In the latter case they are grown on microspheres [1], agarose gel [2], or basement membrane gel [3]. Cell extracts have the advantage of less demanding cultural conditions. One may concentrate the extracts from culture dishes with low cell densities. Extract spectra also have a much higher spectral resolution and better signal-to-noise ratio.


Primary Neuron Creatine Kinase Activity Glia Cell Clonal Cell Line Glutamine Concentration 
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  1. 1.
    Fantini J, Galons JP, Marvaldi J, Cozzone PJ, Canioni P (1987) Growth of a human colonic adenocarcinoma cell line (HT29) on microcarrier beads: metabolic studies by 31phosphorus nuclear magnetic resonance spectroscopy. Int J Cancer 39: 225–260Google Scholar
  2. 2.
    Cohen JS, Lyon RC, Chen C, Faustino PJ, Batist G, Shoemaker M, Rubalcaba E, Cowan KH (1986) Differences in phosphate metabolite levels in drug-sensitive and–resistant human breast cancer cell lines determined by 31P magnetic resonance spectroscopy. Cancer Res 46: 4087–4090PubMedGoogle Scholar
  3. 3.
    Daly PF, Lyon R, Straka EJ, Cohen JS (1988) 31P-NMR spectroscopy of human cancer cells proliferating in a basement membrane gel. FASEB J 2: 2596–2604Google Scholar
  4. 4.
    Benda P, Lightbody J, Sato G, Levine L, Sweet W, (1968) Differentiated rat glial cell strain in tissue culture. Science 161: 370PubMedCrossRefGoogle Scholar
  5. 5.
    Nelson PG, Lieberman M (1981) Exitable cells in tissue culture. Plenum, New York, pp 173–245Google Scholar
  6. 6.
    Richter-Landsberg C (1988) NILE glycoprotein in developing rat cerebral cells in culture. Cell Tissue Res 252: 181–190PubMedCrossRefGoogle Scholar
  7. 7.
    Möller A (1989) Untersuchungen über das Enzym Creatinkinase and sein Substrat Creatin an glialen and neuronalen Zellkulturen des Gehirns. Dissertation, University of TübingenGoogle Scholar
  8. 8.
    Möller A, Hamprecht B (1989) Creatine transport in cultured cells of rat and mouse brain. Neurochemistry 52: 544–550CrossRefGoogle Scholar
  9. 9.
    Birken DL, Oldendorf WH (1989) N-Acetyl-L-aspartic acid: a literature review of a compound prominent in 1H-NMR spectroscopic studies of brain. Neurosci Biobehav Rev 13: 23–31PubMedCrossRefGoogle Scholar
  10. 10.
    Barany M, Arus C, Yen-Chung Chang (1985) Natural-abundance 13C NMR of brain. Magn Reson Med 2: 289–295PubMedCrossRefGoogle Scholar
  11. 11.
    Bradford HF (1986) Chemical neurobiology. Freeman, New YorkGoogle Scholar
  12. 12.
    Jans AWH, Leibfritz D (1989) A C-13 NMR study on fluxes into the Krebs cycle of rabbit renal proximal tubular cells. NMR Biomed 1: 171–176PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • D. Leibfritz
  • A. Brand
  • C. Richter-Landsberg
    • 1
  1. 1.University of BremenBremen 33Germany

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