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13C and 1H Magnetic Resonance Studies of Normal and Neoplastic Brain Cells

  • D. Leibfritz
  • A. Brand
  • C. Richter-Landsberg
Conference paper

Abstract

Current volume-selective in vivo magnetic resonance (MR) spectroscopy in humans records signals from volumes larger than 1 cm3. In this case, the spectra contain peaks from various cell types, which is particularly true for organs such as the brain and kidney. Cell cultures are a means of gaining access to the metabolism of individual cells. As MR requires a fairly large number of cells (approximately 108) two strategies may be employed: either cells are grown in several dishes under optimal conditions and then harvested and extracted, or, if enough cells can be supplied adequately in a MR tube, their metabolism is followed in vivo. In the latter case they are grown on microspheres [1], agarose gel [2], or basement membrane gel [3]. Cell extracts have the advantage of less demanding cultural conditions. One may concentrate the extracts from culture dishes with low cell densities. Extract spectra also have a much higher spectral resolution and better signal-to-noise ratio.

Keywords

Primary Neuron Creatine Kinase Activity Glia Cell Clonal Cell Line Glutamine Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • D. Leibfritz
  • A. Brand
  • C. Richter-Landsberg
    • 1
  1. 1.University of BremenBremen 33Germany

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