13C and 1H Magnetic Resonance Studies of Normal and Neoplastic Brain Cells
Current volume-selective in vivo magnetic resonance (MR) spectroscopy in humans records signals from volumes larger than 1 cm3. In this case, the spectra contain peaks from various cell types, which is particularly true for organs such as the brain and kidney. Cell cultures are a means of gaining access to the metabolism of individual cells. As MR requires a fairly large number of cells (approximately 108) two strategies may be employed: either cells are grown in several dishes under optimal conditions and then harvested and extracted, or, if enough cells can be supplied adequately in a MR tube, their metabolism is followed in vivo. In the latter case they are grown on microspheres , agarose gel , or basement membrane gel . Cell extracts have the advantage of less demanding cultural conditions. One may concentrate the extracts from culture dishes with low cell densities. Extract spectra also have a much higher spectral resolution and better signal-to-noise ratio.
KeywordsLactate Proline Pyruvate Glutamine Alanine
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