High-Field 31P Magnetic Resonance Studies for Evaluating Kidney Viability

  • S. Pomer
  • W. E. Hull
Conference paper


The application of 31P magnetic resonance (MR) to provide a noninvasive evaluation of renal phosphorus metabolism is well established [7, 13, 14]. In a variety of 31P MR studies of excised or perfused kidneys initial attention has been given to determining the time course of nucleotide (NTP + NDP) depletion (sum of all nucleoside 5’-tri- and diphosphates, primarily ATP and ADP) and intrarenal acidosis during cold and warm ischemia [9]. Within a few minutes depletion of intrarenal NTP was noted, and a rapid onset of acidosis was observed. Previous investigations using low-field 31P MR of the isolated perfused kidney demonstrated sequential changes in phosphate components during ischemia [9]. However, the information provided about the components of different signal groups was insufficient; the renal 31P MR spectra obtained at field strengths lower than 4.7 T did not allow adequate sensitivity and resolution for many of the individual phosphate metabolites, for example, detection of phosphocreatine (PCr), separation of NTP and NDP, resolution of phosphomonoesters (PME) into phosphocholine (PC) and phosphoethanolamine (PE) and of diesters (PDE) into glycerophosphocholine (GPC) and glycerophosphoethanolamine (GPE). Through the use of very high field strengths (>7 T) these problems can be solved, at least for ex vivo studies.


Cold Storage Warm Ischemia Cold Ischemia Left Renal Artery Magnetic Resonance Measurement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bretan PN, Vigneron DB, Hricak H, Juenemann K-P, Williams RD, Tanagho EA, James TL (1986) Assessment of renal preservation by phosphorus-31 magnetic resonance spectroscopy: in vivo normothermic blood perfusion. J Urol 136: 1356–1359PubMedGoogle Scholar
  2. 2.
    Bretan PN Jr, Vigneron DB, James TL, Williams RD, Hricak H, Juenemann K-P, Yen TSB, Tanagho EA (1986) Assessment of renal viability by phosphorus-31 magnetic resonance spectroscopy. J Urol 135: 866–871PubMedGoogle Scholar
  3. 3.
    Bretan PN Jr, Baldwin N, Novick AC et al. (1989) Pretransplant assessment of renal viability by phosphorus-31 magnetic resonance spectroscopy. Transplantation 48: 48–53PubMedCrossRefGoogle Scholar
  4. 4.
    Gonzales-Mendez R, Nabsath DC, Stevenson DK, Goms ML, Schulz TE, Jardetzky O (1989) Renal injury and regeneration in adult rats after total ischemia, MRS correlations. Magn Reson Med Biol 2: 21–32Google Scholar
  5. 5.
    Hull WE, Pomer S, Vogt P (1987) High-field 31P-NMR analysis of phosphate metabolites in excised rat kidney: assessment of preservation for transplantation. Abstr Soc Magn Reson Med 6: 281Google Scholar
  6. 6.
    Morris GA, Freeman RA (1978) Selective excitation in Fourier transform NMR. J Magn Reson 29: 433–462Google Scholar
  7. 7.
    Ross B, Freeman D, Chan L (1986) Contributions of nuclear magnetic resonance to renal biochemistry. Kidney Int 29: 131–141PubMedCrossRefGoogle Scholar
  8. 8.
    Seelig J (1978) P-31 NMR and the head group structure of phospholipids in membranes. Biochim Biophys Acta 515: 105–140PubMedGoogle Scholar
  9. 9.
    Sehr PA, Bore PJ, Papatheofanis J, Radda GK (1979) Non-destructive measurement of metabolites and tissue pH in the kidney by 31P nuclear magnetic resonance. Br J Exp Pathol 60: 632–641PubMedGoogle Scholar
  10. 10.
    Siegel NJ, Avison MJ, Reilly HF, Alger JR, Shulman RG (1983) Enhanced recovery of renal ATP with post-ischemic infusion of ATP-MgC12 determined by 31P-NMR. Am J Physiol 245: F530–534PubMedGoogle Scholar
  11. 11.
    Stromski ME, Cooper K, Thulin G, Gaudio GM, Siegal NJ, Shulman RG (1986) Chemical and functional correlates of post-ischemic renal ATP levels. Proc Nail Acad Sci USA 83: 6142–6145CrossRefGoogle Scholar
  12. 12.
    Thoma WJ, Snyder JA, Pearson GA (1989) Broad resonance-edited 31P spectra obtained with a single radiofrequency channel. NMR Biomed 2: 112–114PubMedCrossRefGoogle Scholar
  13. 13.
    Weiner MW, Adam WR (1985) MRS for evaluation of renal function. Semin Urol III: 34–42Google Scholar
  14. 14.
    Wong GG, Ross BD (1983) Application of phosphorus nuclear magnetic resonance to problems of renal physiology and metabolism. Miner Electrolyte Metab 9: 282–289PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • S. Pomer
    • 1
  • W. E. Hull
    • 2
  1. 1.Department of UrologyUniversity ClinicHeidelbergGermany
  2. 2.Central Spectroscopy DepartmentGerman Cancer Research CenterHeidelbergGermany

Personalised recommendations