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EJB Reviews pp 165-179 | Cite as

Concepts and strategies for human gene therapy

  • Klaus Roemer
  • Theodore Friedmann
Part of the EJB Reviews book series (EJB REVIEWS, volume 1992)

Abstract

Beginning in the late 1960s and early 1970s, the availability of new molecular-biological tools and concepts began to suggest that designed genetic changes may eventually provide a new and effective form for the therapy of human diseases. In 1972, the medical need, as well as the technical and conceptual issues involved in efficient virally mediated gene transfer for therapeutic purposes, were outlined (Friedmann and Roblin, 1972). In the 20 years since that time, progress has been so great that human experiments are now underway to test the efficacy of foreign genes in defective human cells as a form of gene therapy. There are a very large number of human diseases that represent potential targets for this kind of manipulation. More than 4500 human diseases are currently classified as genetic (McKusick, 1988). Until now, only a very small minority of these diseases has been associated with specific mutations in the human genome. Recessive genetic diseases like cystic fibrosis and adenosine deaminase (ADA) deficiency require mutations to be present in both alleles of a gene in order to generate the disease phenotype, while dominant diseases like Huntington’s disease can be caused by the presence of only one mutated copy of a gene. The mere presence of a mutant allele overrides the remaining normal allele and leads to disease. With the application of the tools of molecular genetics, and with new approaches to the identification and characterization of disease-related defects, therapy through the correction of genetic defects has come within reach (Friedmann, 1989; Anderson 1992). Most modern gene-therapeutic approaches are based on the introduction of functional copies of defective genes into cells.

Keywords

Gene Therapy Long Terminal Repeat Adenosine Deaminase Latency Associate Transcript Internal Promoter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

HIV

human immunodeficiency virus

AIDS

acquired immune-deficiency syndrome

ADA

adenosine deaminase

LDL

low-density lipoproteins

CFTR

cystic-fibrosis transmembrane-conductance regulator

LTR

long terminal repeats

MDR

multidrug resistance

HSV

herpes simplex virus.

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Copyright information

© Federation of European Biochemical Societies 1993

Authors and Affiliations

  • Klaus Roemer
    • 1
  • Theodore Friedmann
    • 1
  1. 1.Center for Molecular GeneticsUniversity of CaliforniaSan DiegoUSA

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