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Regeneration of Plants from Protoplasts of Some Stone Fruits (Prunus spp.)

  • S. J. Ochatt
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)

Abstract

The genus Prunus (Prunoideae, x = 8; Rosaceae), including all the top-fruit tree species horticulturally known as stone fruits (peach, plum, prune, apricot, cherry), is characterized by its rather complex phylogenesis. This genus can be subdivided into several sections, each including at least one economically important member (Rehder 1967). Most cultivated stone-fruit trees are of a composite nature: the fruitbearing scion and a suitable rootstock (belonging either to the same species or to a closely related one, within the Prunoideae) onto which the scion is grafted in order to provide desirable tree size control and fruit cropping. The history of Prunus species goes back to the Mesolithic period, when prehistoric lake and cave dwellers ate their fruits, while they appear to have been first cultivated in Mesopotamia, as described by Herodotus, the Greek historian, in 500 B.C. Presently, stone fruits are cultivated in all temperate areas of the world.

Keywords

Mesophyll Protoplast Sour Cherry Protoplast Isolation Stone Fruit Prunus Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Chiariotti A (1991) Protoplast isolation from leaf mesophyll and cell suspension cultures of fruit trees. Physiol Plant 82:A16Google Scholar
  2. David H, Domon JM, Savy CS, Miannay N, Sulmont G, Dargent R, David A (1992) Evidence for early stages of somatic embryo development in a protoplast-derived cell culture of Prunus avium. Physiol Plant 85:301–307CrossRefGoogle Scholar
  3. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158PubMedCrossRefGoogle Scholar
  4. Hansche PE, Hesse CO, Beutel J, Beres W, Doyle J (1979) The commercial potential of dwarf fruit trees. Calif Agric (Sept): 4–6Google Scholar
  5. James DJ, Wakerell IJ, Passey AJ (1982) Isolation of protoplasts from fruit plant tissues. Rep E Mailing Res Stn for 1981:162–163Google Scholar
  6. James DJ, Passey AJ, Malhotra SB (1984) Isolation and fusion of protoplasts. Rep E Mailing Res Stn for 1983:63–65Google Scholar
  7. Janick J, Moore JN (eds) (1975) Advances in fruit breeding. Purdue University Press, West Lafayette, INGoogle Scholar
  8. Jorgensen J, Binding H (1988) Protoplast culture of woody Rosaceae and a comparison to herbaceous Rosaceae. In: Ahuja MR (ed) Somatic cell genetics of woody plants. Kluwer, Dordrecht, pp 169–172CrossRefGoogle Scholar
  9. Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110CrossRefGoogle Scholar
  10. Lee N, Wetztein HY (1986) Protoplast isolation from peach. In: Somers DA, Gengenbach BG, Biesboer DD, Hackett WP, Green CE (eds) Proc VI Int Congr Plant Tissue and Cell Culture. Univ Minnesota, MN, p 326 (Abstr)Google Scholar
  11. Marino G (1986) Isolation and culture of protoplasts from callus and suspension-cultured cells of Prunus cerasus and Actinidia chinensis. In: Horn W, Jensen CJ, Odenbach W, Schieder O (eds) Genetic manipulation in plant breeding. Proc Int Symp Eucarpia, 8–13 Sept 1985, West Berlin. De Gruyter, Berlin, pp 487–489Google Scholar
  12. Matsuta N, Irabayashi T, Akihama T (1983) Isolation of protoplasts from peach. Jpn J Breed 33:202–203Google Scholar
  13. Matsuta N, Irabayashi T, Akihama T (1984) Callus formation from Prunus lannesiana Wils. protoplasts. Jpn J Breed 34:42–43Google Scholar
  14. Matsuta N, Irabayashi T, Akihama T, Kozaki I (1986) Callus formation from protoplasts of peach cell suspension cultures. Sci. Hortic 28:59–64Google Scholar
  15. Mouras A, Salesses G, Lutz A (1978) Sur l’utilisation des protoplastes en citologie: amélioration d’une méthode récente en vue de l’identification des chromosomes mitotiques des genres Nicotiana et Prunus. Caryologia 31:117–127Google Scholar
  16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  17. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 165:85–87Google Scholar
  18. Ochatt SJ (1990a) Plant regeneration from root callus protoplasts of sour cherry (Prunus cerasus L.). Plant Cell Rep 9:268–271Google Scholar
  19. Ochatt SJ (1990b) Protoplast technology and top-fruit tree breeding. Acta Hortic 280:215–226Google Scholar
  20. Ochatt SJ (1991) Strategies for plant regeneration from mesophyll protoplasts of the recalcitrant fruit and farmwoodland species Prunus avium L. (sweet/wild cherry), Rosaceae. J Plant Physiol 139:155–160Google Scholar
  21. Ochatt S J (1992) The development of protoplast-to-tree systems for Prunus cerasifera and P. spinosa, Rosaceae. Plant Sci 81:253–259Google Scholar
  22. Ochatt S J, Patat-Ochatt EM (1990) Assessments of graft-compatibility of somatic hybrids, Pyrus communis var. pyraster L. ( + ) Prunus avium x pseudocerasus, and species of the subfamilies Pomoideae and Prunoideae, Rosaceae. VII Int Congr Plant Tissue and Cell Culture IAPTC, Amsterdam, p 216 (Abstr)Google Scholar
  23. Ochatt S J, Patat-Ochatt EM (1991) The time-course evolution of viability and competence for growth of woody plant protoplasts following cold-storage. Physiol Plant 82:A16Google Scholar
  24. Ochatt S J, Power JB (1988) An alternative approach to plant regeneration from protoplasts of sour cherry (Prunus cerasus L.). Plant Sci 56:75–79Google Scholar
  25. Ochatt S J, Power JB (1989a) Selection for salt/drought tolerance using protoplast- and explant-derived tissue cultures of Colt cherry (Prunus avium x pseudocerasus). Tree Physiol 5:259–266Google Scholar
  26. Ochatt SJ, Power JB (1989b) Cell wall synthesis and salt (saline) sensitivity of Colt cherry (Prunus avium x pseudocerasus) protoplasts. Plant Cell Rep 8:365–367Google Scholar
  27. Ochatt SJ, Power JB (1991) Plant regeneration from cultured protoplasts of higher plants. In: Moo-Young M, Warren GS, Fowler MW (eds) Comprehensive biotechnology Suppl 2. Pergamon, New York, pp 99–127Google Scholar
  28. Ochatt SJ, Cocking EC, Power JB (1987) Isolation, culture and plant regeneration of Colt cherry (Prunus avium x pseudocerasus) protoplasts. Plant Sci 50:139–143Google Scholar
  29. Ochatt S J, Chand PK, Rech EL, Davey MR, Power JB (1988a) Electroporation-mediated improvement of plant regeneration from Colt cherry (Prunus avium x pseudocerasus) protoplasts. Plant Sci 54:165–169Google Scholar
  30. Ochatt SJ, Rech EL, Davey MR, Power JB (1988b) Long-term effect of electroporation on enhancement of growth and plant regeneration of Colt cherry (Prunus avium x pseudocerasus) protoplasts. Plant Cell Rep 7:393–395Google Scholar
  31. Ochatt SJ, Patat-Ochatt EM, Rech EL, Davey MR, Power JB (1989) Somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (Pyj-us communis var. Pyraster L.) and Colt cherry (Prunus avium x pseudocerasus). Theor Appl Genet 78:35–41Google Scholar
  32. Power JB, Davey MR, MacLellan MS, Wilson D (1989) Laboratory manual: plant tissue culture. University of NottinghamGoogle Scholar
  33. Raff J, McKenzie IFC, Clarke AE (1980) Antigenic determinants of Prunus avium are associated with the protoplast surface. Z Pflanzenphysiol 98:225–234Google Scholar
  34. Rech EL, Ochatt SJ, Chand PK, Power JB, Davey MR (1987) Electro-enhancement of division of protoplast-derived cells. Protoplasma 141:169–176Google Scholar
  35. Rech EL, Ochatt S J, Chand PK, Mulligan BJ, Davey MR, Power JB (1988) Electroporation increases DNA synthesis in cultured plant protoplasts. Biotechnology 6:1091–1093Google Scholar
  36. Rehder A (1967) Manual of cultivated trees and shrubs, 2nd edn. MacMillan, New YorkGoogle Scholar
  37. Revilla MA, Ochatt SJ, Doughty D, Power JB (1987) A general strategy for the isolation of leaf mesophyll protoplasts from deciduous fruit and nut trees. Plant Sci 50:133–137CrossRefGoogle Scholar
  38. Rugini E (1987) Almond. In: Vasil IK (ed) Cell culture and somatic cell genetics, vol 4. Academic Press, New York, pp 574–611Google Scholar
  39. Salesses G, Mouras A (1977) Tentative d’utilisation des protoplastes pour l’étude des chromosomes chez les Prunus. Ann Amélior Plant 27:363–368Google Scholar
  40. Sansavini S (1987) Current trends in stone fruit growing in Europe. Fruit Var J 41:114–128Google Scholar
  41. Weinbaum SA, Labavitch JM, Weinbaum Z (1979) The influence of ethylene treatment of immature fruit of prune (Prunus domestica L.) on the enzyme-mediated isolation of mesocarp cells and protoplasts. J Am Soc Hortic Sci 104:278–280Google Scholar
  42. Wu SC, Kuniyuki AH (1985) Isolation and culture of almond protoplasts. Plant Sci 41:55–60CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • S. J. Ochatt
    • 1
  1. 1.Station d’Amélioration des Espèces Fruitières et OrnamentalesI.N.R.A. Centre de Recherches d’AngersBeaucouzéFrance

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