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Regeneration of Plants from Protoplasts of Adsuki Bean (Phaseolus angularis Wight)

  • K. L. Ge
  • Y. Z. Wang
  • X. M. Yuan
  • P. M. Huang
  • J. S. Yang
  • J. Q. Huang
  • C. C. Tan
  • N. Lee
  • D. Testa
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)

Abstract

Plant regeneration from protoplasts is a prerequisite in protoplast technology. In this respect, however, little information is available for grain legume plants. In the genus Phaseolus, which has sometimes been classified under the genus Vigna, belonging to the family Leguminosae, calli were obtained from protoplasts of Phaseolus aureus (Xu et al. 1981) and P. vulgaris (Pelcher et al. 1974). However, no plantlets have been regenerated from them. Later, plants were regenerated from protoplasts of Vigna acontifolia (Gill and Eapen 1986). Since 1984, on the basis of plantlet regeneration from explants of P. angularis, which is indigenous to China and which has been cultivated as an important feed crop for at least 2000 years, we proceeded to carry out experiments in our laboratory, with protoplast cultures from sterilized leaves of this plant, and obtained vigorous calli and induced plantlet regeneration.

Keywords

Callus Formation Plantlet Regeneration Mesophyll Protoplast Protoplast Culture Plating Efficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

6BA

6-benzylaminopurine

2,4-D

2,4-dichlorophenoxyacetic acid

GA3

gibberrellin

MS

Murashige and Skoog’s medium

NAA

alpha-naphthalene acetic acid

zeatin

6-4(4-hydroxy-3-methylbut-2-enylamino)-purine

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • K. L. Ge
    • 1
  • Y. Z. Wang
    • 1
  • X. M. Yuan
    • 2
  • P. M. Huang
    • 1
  • J. S. Yang
    • 1
  • J. Q. Huang
    • 1
  • C. C. Tan
    • 1
  • N. Lee
    • 2
  • D. Testa
    • 2
  1. 1.Institute of GeneticsFudan UniversityShanghaiChina
  2. 2.Interferon Sciences, IncNew BrunswickUSA

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