Transformation of Stylosanthes Species

  • A. R. Elliott
  • J. M. Manners
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)


Stylosanthes spp. are pasture legumes grown in tropical and subtropical areas in South-east Asia, Africa, South America and Australia (Burt et al. 1983). The genus Stylosanthes represents the most important pasture legumes of northern Australia and it is estimated that currently 700 000 ha are sown in this region, with potential for future expansion to 18.3 million ha (Weston et al. 1981). Stylosanthes spp. are mainly oversown into native grass pastures to increase the productivity of areas of low fertility (Walker and Weston 1990).


Transgenic Plant Hairy Root Agrobacterium Rhizogenes Colletotrichum Gloeosporioides Pasture Legume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. An G, Watson BD, Chiang CC (1986) Transformation of tobacco tomato, potato and Arabidopsis thaliana using a binary Ti vector system. Plant Physiol 81:301–305PubMedCrossRefGoogle Scholar
  2. Anderson AR, Moore LW (1979) Host specificity in the genus Agrobacterium. Phytopathology 69:320–323CrossRefGoogle Scholar
  3. Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H (1982) Nucleotide sequence and exact location of the neomycin phosphotransferase gene from transposon Tn5. Gene 19:327–336PubMedCrossRefGoogle Scholar
  4. Begg JE (1972) Probable distribution of Townsville stylo as a naturalised legume in tropical Australia. J Aust Inst Agric Sci 38:158–162Google Scholar
  5. Benfey PN, Ren L, Chua N-H (1990) Tissue-specific Expression from CaMV 35S enhancer subdomains in early stages of plant development. EMBO J 9:1677–1684PubMedGoogle Scholar
  6. Bevan M, Shufflebottom D, Edwards K, Jefferson R, Schuch W (1989) Tissue- and cell-specific activity of phenylalanine ammonia-lyase promoter in transgenic plants. EMBO J 8:1899–1906PubMedGoogle Scholar
  7. Bohlmann H, Apel K (1991) Thionins. Annu Rev Plant Physiol 42:227–240CrossRefGoogle Scholar
  8. Brown AE, Davis RD (1992) Chitinase activity in Stylosanthes guianensis systemically protected against Colletotrichum gloeosporioides. J. Phytopathology 136:247–256.CrossRefGoogle Scholar
  9. Burt RL, Cameron DG, Cameron DF, ’t Mannetje L, Lenne J (1983) Stylosanthes. In: Burt RL, Rotar PP, Walker JL, Silvey MW (eds) The role of Centrosema, Desmodium, and Stylosanthes in improving tropical pastures. West view Press, Boulder, pp 141–185Google Scholar
  10. Byrne MC, McDonnell RE, Wright MS, Carnes MG (1987) Strain and cultivar specificity in the Agrobacterium-soybean interaction. Plant Cell Tissue Organ Cult 8:3–15CrossRefGoogle Scholar
  11. Cameron DF, Chakraborty S, Davis S, Edye LA, Irwin JAG, Manners JM, Staples IB (1989) A multidisciplinary approach to anthracnose diseases of Stylosanthes in Australia. In: Desroches R (ed) Proc XVI Int Grassland Congr, Nice, French Grassland Society, Versailles-en-Cedex, pp 719–720Google Scholar
  12. Castresana C, de Carvalho F, Gheysen G, Habets M, Inze D, Van Montagu M (1990) Tissue-specific and pathogen-induced regulation of a Nicotiana plumbaginifolia fi-1, 3-glucanase gene. Plant Cell 2:1131–1143Google Scholar
  13. Chet I, Broglie K, Broglie R (1990) Biotechnology and biocontrol of fungi. Abstr IIE-225/3 of the 4th Int Mycological Congr, Regensberg, GermanyGoogle Scholar
  14. Edye LA, Hall TJ, Middleton CH, Messer WB, Piggin CM, Schlink AC, Klepacki NM (1991) Sward evaluation of fifteen Stylosanthes hamata accessions in twenty dry tropical environments. Trop Grassl 25:1–11Google Scholar
  15. Franks T, Birch RG (1991) Gene transfer into intact sugarcane cells using microprojectile bombardment. Aust J Plant Physiol 18:471–480Google Scholar
  16. Golds TJ, Lee JY, Husnain T, Ghose TK, Davey MR (1991) Agrobacterium rhizogenes mediated transformation of forage legumes Medic ago sativa and Onobrychis viciifolia. J Exp Bot 42:1147–1157Google Scholar
  17. Hain R, Stabel P, Czernilofsky AP, SteinbiB HH, Herrera-Estrella L, Schell J (1985) Uptake, integration, expression and genetic transmission of a selectable chime’ric gene by plant protoplasts. Mol Gen Genet 199:166–168Google Scholar
  18. Harkin JM (1973) Lignin. In: Butler GW, Bailey RW (eds) Chemistry and biochemistry of herbage, vol 1. Academic Press, New York, pp 323–373Google Scholar
  19. Hawes MC, Robbs SL, Pueppke SG (1989) Use of a root tumorigenesis assay to detect genotypic variation in susceptibility of thirty-four cultivars of Pisum sativum to crown gall. Plant Physiol 90:180–184Google Scholar
  20. Hobbs SLA, Jackson J A, Mahon JD (1989) Specificity of strain and genotype in the susceptibility of pea to Agrobacterium tumefaciens. Plant Cell Rep 8:274–277Google Scholar
  21. Hobbs SLA, Kpodar P, DeLong CMO (1990) The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol 15:851–864Google Scholar
  22. Hoekema A, Hooykaas PJJ, Schilperoort RA (1984) Transfer of the octopine T-DNA segment to plant cells mediated by different types of Agrobacterium tumor or root-inducing plasmids: generality of virulence systems. J Bacteriol 158:383–385Google Scholar
  23. Hood EH, Fraley RT, Chiltern M-D (1987) Virulence of Agrobacterium tumefaciens strain A281 on legumes. Plant Physiol 83:529–534Google Scholar
  24. Jefferson RA (1987) Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep 5:387–405Google Scholar
  25. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene marker in higher plants. EMBO J 6:3901–3907Google Scholar
  26. Kado CI (1991) Molecular mechanisms of crown gall tumorigenesis. Crit Rev Plant Sci 10:1–32Google Scholar
  27. Klaas M, John MC, Crowell DN, Amasino RM (1989) Rapid induction of genomic demethylation and T-DNA gene expression in plant cells by 5-azacytisine derivatives. Plant Mol Biol 12:413–423Google Scholar
  28. Klein TM, Wolf ED, Wu R, Sanford JC (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327:70–73Google Scholar
  29. Kincz C, Martini N, Mayerhofer R Koncz-Kalman Z, Korber H, Redei GP, Schell J (1989) High frequency T-DNA-mediated gene tagging in plants. Proc Natl Acad Sci USA 86:8467–8471Google Scholar
  30. Lyznik LA, Ryan RD, Ritchie SW, Hodges TK (1989) Stable co-transformation of maize protoplasts with gusA and neo genes. Plant Mol Biol 13:151–161Google Scholar
  31. Manners JM (1987) Transformation of Stylosanthes spp. using Agrobacterium tumefaciens. Plant Cell Rep 6:204–207Google Scholar
  32. Manners JM (1988) Transgenic plants of the tropical pasture legume Stylosanthes humilis. Plant Sci 55:61–68Google Scholar
  33. Manners JM, Way H (1989) Efficient transformation with regeneration of the tropical pasture legume Stylosanthes humilis using Agrobacterium rhizogenes and a Ti plasmid-binary vector system. Plant Cell Rep 8:341–345Google Scholar
  34. Manners JM, Masel A, Braithwaite KS, Irwin JAG (1992) Molecular analysis of Colletotrichum gloeosporioides pathogenic on the tropical pasture legume Stylosanthes. In Bailey JA, Jeger M (eds) Colletotrichum: Biology, Pathology and Control. CAB International, Oxford pp. 250–268Google Scholar
  35. Mariotti D, Davey MR, Draper J, Freeman JP (1984) Crown gall tumorigenesis in the forage legume Medicago sativa L. Plant Cell Physiol 25:473–482Google Scholar
  36. Matzke MA, Matzke AJM (1991) Differential inactivation of a transgene in plants by two suppressor loci containing homologous sequences. Plant Mol Biol 16:821–830Google Scholar
  37. Mauch F, Mauch-Mani B, Boiler T (1988) Anti-fungal hydrolases in pea tissue II. Inhibition of fungal growth by combinations of chitinase and /-1,3-glucanase. Plant Physiol 88:936–942Google Scholar
  38. Meijer EGM (1982) High-frequency plant regeneration from hypocotyl- and leaf-derived tissue cultures of the tropical pasture legume Stylosanthes humilis. Physiol Plant 56:381–385Google Scholar
  39. Meijer EGM, Steinbiss HH (1983) Plantlet regeneration from suspension and protoplast cultures of the tropical pasture legume Stylosanthes guianensis (Aubl.) Sw. Ann Bot 52:305–310Google Scholar
  40. Meijer EGM, Szabados L (1990) Cell and tissue culture of Stylosanthes spp. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 10. Legumes and oilseed crops I. Springer, Berlin Heidelberg New York, pp 312–322Google Scholar
  41. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  42. Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimaeric chalcone synthase gene into petunia results in reversible co-suppression of homologous gene in trans. Plant Cell 2:219–289Google Scholar
  43. Neuhaus JM, Ahl-Goy P, Hinz U, Flores S, Meins F (1991) High-level expression of a tobacco chitinase gene in Nicotiana sylvestris. Susceptibility of transgenic plants to Cercospora nicotianae infection. Plant Mol Biol 16:141–151Google Scholar
  44. Puonti-Kaerlas J, Stable P, Eriksson T (1989) Transformation of pea (Pisum sativum L.) by Agrobacterium tumefaciens. Plant Cell Rep 8:321–324Google Scholar
  45. Ramsay G, Kumar A (1990) Transformation of Viciafabacotyledon and stem tissues by Agrobacterium rhizogenes: infectivity and cytological studies. J Exp Bot 41:841–847Google Scholar
  46. Rathus C, Birch RG (1992) Stable transformation of callus from electroporated sugarcane protoplasts. Plant Sci 82:81–89Google Scholar
  47. Riggs CD, Bates GW (1986) Stable transformation of tobacco by electroporation: evidence for plasmid concatenation. Proc Natl Acad Sci USA 83:5602–5606Google Scholar
  48. Sakellaris H, Pemberton JM, Manners JM (1990) Genes from Cellvibrio mixtus encoding endoglucanase. Appl Environ Microbiol 56:3204–3208Google Scholar
  49. Sanders PR, Winter JA, Barnason AR, Rogers SG, Fraley RT (1987) Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants. Nucleic Acids Res 15:1543–1558Google Scholar
  50. Sarria R, Calderon A, Thro AM, Roca WM (1991) Efficient genetic transformation of the tropical forage legume Stylosanthes guianensis using a binary Agrobacterium tumefaciens vector. Abstr 361 of the 3rd Int Congr Plant Mol Biol, Tucson, ArizonaGoogle Scholar
  51. Scheid OM, Paszkowski J, Potrykus I (1991) Reversible inactivation of a transgene in Arabidopsis thaliana. Mol Gen Genet 228:104–112Google Scholar
  52. Shimoda N, Toyoda-Yamamoto A, Nagamine J, Usami S, Katayama M, Sakagami Y, Machida Y (1990) Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides. Proc Natl Acad Sci USA 87:6684–6688Google Scholar
  53. Spano L, Mariotti D, Cardarelli M, Branca C, Costantino P (1988) Morphogenesis and auxin sensitivity of transgenic tobacco with different compliments of Ri T-DNA. Plant Physiol 87:479–483Google Scholar
  54. Stace HM, Cameron DF (1984) Cytogenetics and the evolution of Stylosanthes. In: Stace HM, Edye LA (eds) The biology and agronomy of Stylosanthes. Academic Press, London, pp 49–72Google Scholar
  55. Szabados L, Roca WM (1986) Regeneration of isolated mesophyll and cell suspension protoplasts to plants in Stylosanthes guianensis. Plant Cell Rep 5:174–177Google Scholar
  56. Tepfer D (1984) Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 37:959–967Google Scholar
  57. Topping JF, Wei W, Lindsey K (1991) Functional tagging of regulatory elements in the plant genome. Development 112:1009–1019PubMedGoogle Scholar
  58. Vieira MLC, Jones B, Cocking EC, Davey MR (1990) Plant generation from protoplasts isolated from seedling cotyledons of Stylosanthes guianensis, S. macrocephala and S. scabra. Plant Cell Rep 9:289–292Google Scholar
  59. Vigers AJ, Roberts WK, Selitrennikoff CP (1991) A new family of plant antifungal proteins. Mol Plant-Microbe Interact 4:315–323PubMedCrossRefGoogle Scholar
  60. Walker B, Weston EJ (1990) Pasture development in Queensland: a success story. Trop Grassl 24:257–268Google Scholar
  61. Watson JM (1990) Genetic engineering of low-lignin pasture plants. In: Akin DE, Ljungdahl LG, Wilson JR, Harris PJ (eds) Proc Tri-National Workshop on Microbial and plant opportunities to improve lignoeellulose utilisation by ruminents. Elsevier, New York, pp 215–226Google Scholar
  62. Weston EJ, Harbison J, Leslie JK, Rosenthal KM, Mayer RJ (1981) Assessment of agriculture and pasture potential of Queensland. Queensland Department of Primary Industries, Brisbane, Australia, 195 ppGoogle Scholar
  63. Woloshuk CP, Meulenhoff JS, Sela-Buurlage M, van den Elzen PJM, Cornelissen BJC (1991) Pathogen-induced proteins with inhibitory activity toward Phytophthora infestans. Plant Cell 3:619–628CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • A. R. Elliott
    • 1
  • J. M. Manners
    • 2
  1. 1.Department of BotanyThe University of QueenslandBrisbaneAustralia
  2. 2.CSIRO Division of Tropical Crops and PasturesCunningham LaboratorySt LuciaAustralia

Personalised recommendations