Advertisement

Transformation of Petunia and Corn Plants (Petunia hybrida and Zea mays) Using Agrobacterium tumefaciens and the Shoot Apex

  • J. H. Gould
  • E. C. Ulian
  • R. H. Smith
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)

Abstract

Agrobacterium tumefaciens has evolved a reliable mechanism to transfer DNA into the genome of plant cells and is the most efficient gene vector available. However, the host range of the bacterium has been thought to be restricted to a narrow range of plant species (DeCleene 1985). This range is generally considered to include all dicotyledonous families; however, many dicot species are refractory to infection and members of some monocotyledonous families can be transformed. Furthermore, the apparent requirement for the regeneration of plants from callus or embryogenic callus imposed serious limits to the practical range of plants that could be transformed using A. tumefaciens.

Keywords

Shoot Apex Agrobacterium Tumefaciens Corn Plant Petunia Hybrida Maize Streak Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Braun A (1962) Tumor inception and development in the crown gall disease. Annu Rev Plant Physiol 13:533–558CrossRefGoogle Scholar
  2. Dale PJ, Marks MS, Brown MM, Woolston CJ, Gunn HV, Mullineaux PM, Lewis DM, Kemp JM, Chen DF, Gilmour DM, Flavell RB (1989) Agroinfection of wheat: inoculation of in vitro grown seedlings and embryos. Plant Sei 63:237–245CrossRefGoogle Scholar
  3. DeCleene M (1985) The susceptibility of monocotyledons to Agrobacterium tumefaciens. Phytopathol Z 113:81–89CrossRefGoogle Scholar
  4. Deilaporta SI, Wood J, Hicks JB (1983) A plant DNA minipreparation version II. Plant Mol Biol Reporter 1(4): 19–21CrossRefGoogle Scholar
  5. Feinberg AP, Vogelstein B (1984) A technique for labelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267PubMedCrossRefGoogle Scholar
  6. Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technol 8:833–839CrossRefGoogle Scholar
  7. Gordon-Kamm WJ, Spencer MT, Mangano ML, Adams TR, Daines RJ, Start WG, O’Brien JV, Chambers SA, Adams WR, Willetts NG, Rice TB, Makey CJ, Kruger RW, Kausch AP, Lemax PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2:603–618PubMedCrossRefGoogle Scholar
  8. Gould JH, Devey ME, Hasegawa O, Ulian EC, Peterson G, Smith RH (1991) Transformation of Zea mays L., using Agrobacterium tumefaciens and the shoot apex. Plant Physiol 95:426–434PubMedCrossRefGoogle Scholar
  9. Graves AC, Goldman S (1986) The transformation of Zea mays seedlings with Agrobacterium tumefaciens. Plant Mol Biol 43:50CrossRefGoogle Scholar
  10. Grimsley N, Hohn T, Davis JW, Hohn B (1987) Agrobacterium mediated delivery of infectious maize streak virus into maize plants. Nature 325:177–179CrossRefGoogle Scholar
  11. Hess D, Dressler K, Nimmrichter (1990) Transformation experiments by pipetting Agrobacterium into the spikelets of wheat (Triticum aestivum L.). Plant Sei 72:233–244Google Scholar
  12. Hohn B, Koukolikova-Nicola Z, Bakkeren G, Grimsley N (1989) Agrobacterium-mediated gene transfer to monocots and dicots. Genome 31:987–992Google Scholar
  13. Hood EE, Jen G, Kayes L, Kramer J, Fraley RT, Chilton MD (1984) Restriction endonuclease map of pTiBo542, a potential Ti plasmid vector for genetic engineering of plants. Bio/Technol 2:702–709Google Scholar
  14. Jefferson RA (1988) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405Google Scholar
  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  16. Potrykus I (1990) Gene transfer to cereals: an assessment. Bio/Technol 8:535–542Google Scholar
  17. Raineri DM, Bottino P, Gordon MP and Nester EW (1990) Agrobacterium transformation of rice (Oryza sativa L.). Bio/Technol 8:33–38Google Scholar
  18. Schafer W, Gorz A, Kahl G (1987) T-DNA integration and expression in a monocot crop plant after induction of Agrobacterium. Nature 327:529–531Google Scholar
  19. Smith R, Murashige T (1970) In vitro development of isolated shoot apical meristem of angiosperms. Am J Bot 57:562–568CrossRefGoogle Scholar
  20. Smith R, Murashige T (1982) Primordial leaf and phytohormone effects on excised shoot apical meristems of Coleus blumei Benth. Am J Bot 69:1334–1339CrossRefGoogle Scholar
  21. Stachel SE, Messens E, van Montague M, Zambriski P (1985) Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens. Nature 318:624–629CrossRefGoogle Scholar
  22. Stomp A-M, Loopstra C, Chilton WS, Sederoff RR, Moore LW (1989) Extended host range of Agrobacterium tumefaciens in the genus Pinus. Plant Physiol 92:1226–1232CrossRefGoogle Scholar
  23. Ulian E, Smith R, Gould J, McKnight T (1988) Transformation of plants via the shoot apex. In Vitro Cell Dev Biol 24:951–954CrossRefGoogle Scholar
  24. Veluthambi K, Krishnan M, Gould JH, Smith RH, Gelvin SB (1989) Opines stimulate the induction of the VIR genes of the Agrobacterium tumefaciens Ti plasmid. J Bacteriol 171:3696–3703PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • J. H. Gould
  • E. C. Ulian
  • R. H. Smith
    • 1
  1. 1.Department of Soil & Crop SciencesTexas A & M UniversityCollege StationUSA

Personalised recommendations