Advertisement

Transformation of Tobacco (Nicotiana clevelandii and N. Benthamiana)

  • R. Tavazza
  • G. Ancora
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)

Abstract

A range of procedures has been developed for transferring specific DNA into plant cells. These include the uptake of DNA by protoplasts (Krens et al. 1982; Potrykus et al. 1985), DNA transfer mediated by Agrobacterium, and more recently the microprojectile (Klein et al. 1987) or shoot gun method, which is of great interest for the transformation of plants whose tissues are recalcitrant to regeneration of whole plants from cells.

Keywords

Transgenic Plant Transgenic Tobacco Plant Coat Protein Gene Crown Gall Direct Gene Transfer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adang MJ, Firoozabady E, Klein J, De Boer V, Kemp JD, Murray EE, Rocheleau TA, Rashka K, Staffeld G, Stock C, Sutton D, Merlo DJ (1987) Expression of a Bacillus thuringiensis insecticidal crystal protein gene in tobacco plants. In: Arntzen CJ, Ryan C (eds) Molecular strategies for crop protection. UCLA Symp on Molecular and cellular biology, New Series, vol 46. Liss, New York, pp 345–353Google Scholar
  2. An G (1987) Binary vectors from plant transformation and promoter analysis. Methods Enzymol 153:292–305CrossRefGoogle Scholar
  3. An G, Watson B, Stachel S, Gordon M, Nester E (1985) New cloning vehicles for transformation of higher plants. EMBO J 4:247–284Google Scholar
  4. An G, Watson BD, Ciang CC (1987) Transformation of tobacco, tomato, potato and Arabidopsis thaliana using a binary Ti vector system. Plant Physiol 81:301–305CrossRefGoogle Scholar
  5. Barton KA, Binns AN, Matzke AJ, Chilton MD (1983) Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progeny. Cell 32:1033–1043PubMedCrossRefGoogle Scholar
  6. Barton KA, Whiteley HR, Yang N-S (1987) Bacillus thuringiensis delta endotoxin expressed in Nicotiana tabacum provides resistance to lepidopteran insects. Plant Physiol 85:1103–1109PubMedCrossRefGoogle Scholar
  7. Beachy RN, Loesch-Fries S, Turner NE (1990) Coat protein-mediated resistance against virus infection. Annu Rev Phytopathol 28:451–474CrossRefGoogle Scholar
  8. Benvenuto E, Tavazza R, Ordas RJ, Ancora G, Biocca S, Cattaneo A, Galeffi P (1991) Phytoantibodies: a general vector for the expression of immunoglobulin domains in transgenic plants. Plant Mol Biol 17:865–874PubMedCrossRefGoogle Scholar
  9. Be van MV (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12:8711–8721CrossRefGoogle Scholar
  10. Bevan MW, Mason SE, Goelet P (1985) Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium. EMBO J 4:1921–1926PubMedGoogle Scholar
  11. Budelier KA, Smith AG, Gasser CS (1990) Regulation of a stylar transmitting tissue-specific gene in wild-type and transgenic tomato and tobacco. Mol Gen Genet 224:183–192Google Scholar
  12. Callis J, Raasch J A, Vierstra RD (1990) Ubiquitin extension proteins of Arabidopsis thaliana. Structure, localization, and expression of their promoters in transgenic tobacco. J Biol Chem 265(21): 12486–12493Google Scholar
  13. Chilton MD, Currier T, Farrand S, Bendich A, Gordon MP, Nester EW (1974) Agrobacterium DNA and PS9 bacteriophage DNA not detected in crown gall tumours. Proc Natl Acad Sci USA 71:3672–3676Google Scholar
  14. Comai L, Facciotti D, Hiatt WR, Thompson G, Rose RE, Stalker DM (1985) Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317:741–744Google Scholar
  15. Crossway A, Oakes JV, Irvine JM, Ward B, Knauf VC, Showmaker CK (1986) Integration of foreign DNA following microinjection of protoplast mesophyll protoplasts. Mol Gen Genet 202:179–185Google Scholar
  16. Crossway A, Oakes JV, Irvine JM, Ward B, Knauf VC, Showmaker CK (1986) Integration of foreign DNA following microinjection of protoplast mesophyll protoplasts. Mol Gen Genet 202:179–185Google Scholar
  17. De Block M, Herrera-Hestrella L, Van Montague M, Schell J, Zambryski P (1984) Expression of foreign genes in regenerated plants and in their progeny. EMBO J 3(8): 1681–1689Google Scholar
  18. De Block M, Botterman J, Vandewiele M, Docksx J, Thoen C (1987) Engineering herbicide resistance in plants by expressing of a detoxifying enzyme. EM BO J 6:2513–18Google Scholar
  19. During K, Hippe S, Kreuzaler F, Schell J (1990) Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum. Plant Mol Biol 15:281–293Google Scholar
  20. Fraley RT, Rogers SG, Horsch RB, Sanders P, Flick J, Adams S, Bittner M, Brand L, Fink C, Fray J, Galuppi G, Goldberg S, Hoffmann N, Woo S (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sei USA 80:4803–4807Google Scholar
  21. Fraley RT, Rogers SG, Horsch RB, Eichholts DP, Flick J, Fink C, Hoffmann N, Sanders P (1985) The SEV sistem: a new disarmed Ti plasmid vector for plant transformation. Bio/Technol 3:629–635Google Scholar
  22. Garfinkel DJ, Nester EW (1980) Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J Bacteriol 144:732–743Google Scholar
  23. Guerrero FD, Crossland L, Smutzer GS, Hamilton DA, Mascarenhas JP (1990) Promoter sequences from a maize pollen-specific gene direct tissue-specific transcription in tobacco. Mol Gen Genet 224:161–168Google Scholar
  24. Haughn GW, Smith J, Mazur B, Sommerville C (1988) An Arabidopsis acetolactate synthase gene tobacco confers resistance to sulfonzlurea herbicides. Mol Gen Genet 211(2): 266–271Google Scholar
  25. Hiatt A (1990) Antibodies produced in plants. Nature 344:469–470Google Scholar
  26. Hiatt A (1990) Antibodies produced in plants. Nature 344:469–470Google Scholar
  27. Hilder VA, Gatehouse AMR, Boulter D (1987) A novel mechanism of insect resistance engineered into tobacco. Nature 330:160–163Google Scholar
  28. Horsch RB, Klee HJ (1986) Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: role of the T-DNA borders in the transfer process. Proc Natl Acad Sei USA 83:4428–4432Google Scholar
  29. Horsch RB, Fraley RT, Rogers SG, Sanders PR, Lloyd A, Hoffmann NL (1984) Inheritance of functional foreign genes in plants. Science 223:496–498Google Scholar
  30. Horsch RB, Fraley JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231Google Scholar
  31. Jefferson RA (1988) Plant reporter genes: the GUS gene fusion system. In: Setlow JK (ed) Genetic engineering, vol 10. Plenum, New York, pp 247–263Google Scholar
  32. Kares C, Prinsen E, van Onckelen H, Otten L (1990) IAA synthesis and root induction with iaa genes under heat shock promoter control. Plant Mol Biol 15(2): 225–236Google Scholar
  33. Kiernan JM, Goldberg K-B, Young MJ, Scoelz JE, Shepard RJ (1989) Transformation and regeneration of Nicotiana edwardsonii. Plant Sei 64:61–78Google Scholar
  34. Klein TM, Wolf ED, Wu R, Sanford JC (1987) High velocity microprojectiles for delivering nucleic acids into living cells. Nature 327:70–73Google Scholar
  35. Krens FA, Molendijk L, Wullems GJ, Schilperoort RA (1982) In vitro transformation of plant protoplasts with Ti plasmid DNA. Nature 296:72–74Google Scholar
  36. Laimer A, Da Camara Machado A, Mattanovich D, Regner F, Hanzer V, Durniok B, Steinkellner H, Himmler G, Katinger H (1990) Expression of the plum pox virus coat protein gene in Nicotiana clevelandii. Acta Hort 280:577–580Google Scholar
  37. Lippincott JA, Heberlein GT (1965) The induction of leaf tumours by Agrobacterium tumefaciens. Am J Bot 52:396–399Google Scholar
  38. Llewellyn D, Lyon BR, Cousin Y, Huppatz J, Dennis ES, Peaccock WJ (1990) Genetic engineering of plants for resistance to the herbicide 2,4-D. In: Lycett, Grierson D (eds) Genetic engineering of crop plants. Butterworths, London, pp 67–77Google Scholar
  39. Marton L, Wullems GJ, Molendijk L, Schilperoort RA (1979) In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens. Nature 277:129–131Google Scholar
  40. Mattanovich D, Ruker F, Da Camara Machado A, Laimer M, Regner F, Steinkellner H, Himmler G, Katinger H (1989) Efficient transformation of Agrobacterium spp. by electroporation. Nucleic Acids Res 17(16): 6747–6757Google Scholar
  41. Murai N, Sutton D, Murray M, Slighton T, Merlo D, Reichert N, Sengupta-Gopalan C, Stock C, Baeker R, Kemp J, Hall T (1983) Phaseolin gene from bean is expressed after transfer to sunflower via tumor-inducing plasmid vectors. Science 222:476–482Google Scholar
  42. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497Google Scholar
  43. Murray EE, Rocheleau T, Eberle M, Stock C, Sekar V, Adang M (1991) Analysis of unstable RNA transcript of insecticidal crystal protein genes of Bacillus thuringiensis in transgenic plants and electroporated protoplasts. Plant Mol Biol 16:1035–1050Google Scholar
  44. Negrutiu I, Shillito R, Potrykus I, Biasini G, Sala F (1987) Hybrid genes in the analysis of transformation conditions. Setting up a simple method for direct gene transfer in plant protoplasts. Plant Mol Biol 8:363–373Google Scholar
  45. Nelson RS, Powel-Abel P, Beachy RN (1987) Lesion and virus accumulation in inoculated transgenic tobacco plants expressing the coat protein gene of tobacco mosaic virus. Virology 158:126–132Google Scholar
  46. Otten L, De Greve H, Hernalsteens JP, Van Montagu M, Schieder O, Straub J, Schell J (1981) Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens. Mol Gen Genet 183:209–213Google Scholar
  47. Ow DW, Wood KW, De Luca M, De Wet JR, Helinski DR, Howell SH (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234:856–859Google Scholar
  48. Paszkowski J, Shillito RD, Mandak SM, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3:2717–2722Google Scholar
  49. Potrykus I, Shillito RD, Saul MW, Paszkowski J (1985) Direct gene transfer: state of the art and future potential. Plant Mol Rep 3:117–128Google Scholar
  50. Powel-Abel P, Nelson RS, De B, Hoffman N, Rogers SG, Fraley RT (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–743Google Scholar
  51. Riggs CD, Bates GW (1986) Stable transformation of tobacco by electroporation: evidence for plasmid concentration. Proc Natl Acad Sci USA 83:5602–5606Google Scholar
  52. Roby B, Broglie K, Cressman R, Biddle P, Chet I, Broglie R (1990) Activation of a bean chitinase promoter in transgenic tobacco plants by phytopathogenic fungi. Plant Cell 2:999–1007Google Scholar
  53. Rogers SG, Horsch RB, Fraley RT (1986) Gene transfer in plants: production of transformed plants using Ti plasmid vectors, Methods Enzymol 118:627–640Google Scholar
  54. Schindler U, Cashmore AR (1990) Photoregulated gene expression may involve ubiquitous DNA binding proteins. EMBO J 9:3415–3427Google Scholar
  55. Scott Elmer J, Sunter G, Gardiner WE, Brand L, Browning CK, Bisaro DM, Rogers SG (1988) Agrobacterium-mediated inoculation of plants with tomato golden mosaic virus DNAs. Plant Mol Biol 10:225–234CrossRefGoogle Scholar
  56. Stanley J, Frischmuth T, Ellwood S (1990) Defective viral DNA ameliorates symptoms of geminivirus infection in transgenic plants. Proc Natl Acad Sci USA 87:6291–6295PubMedCrossRefGoogle Scholar
  57. Tavazza R, Ordas RJ, Tavazza M, Ancora G and Benvenuto E (1988) Genetic transformation of Nicotiana clevelandii using a Ti plasmid vector. J Plant Physiol 133:6Google Scholar
  58. Vaeck M, Reynaerts A, Hofte H, Jansens S, De Beukleer MD, Dean C (1987) Transgenic plants protected from insect attack. Nature 328:33–37CrossRefGoogle Scholar
  59. Weising K, Schell J, Kahl G (1988) Foreign genes in plants: transfer, structure, expression and applications. Annu Rev Genet 22:421–477PubMedCrossRefGoogle Scholar
  60. Wullems GJ, Molendijk L, Ooms G, Schilperoort RA (1981) Differential expression of crown gall tumor markers in transformants obtained after in vitro Agrobacterium tumefaciens-induced transformation of cell wall regenerating protoplasts derived from Nicotiana tabacum. Proc Natl Acad Sci USA 78:4344–4348PubMedCrossRefGoogle Scholar
  61. Zambryski P, Joos H, Genetello C, Leemans J, van Montague M, Schell J (1983) Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal generation capacity. EMBO J 2:2143–2150PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • R. Tavazza
  • G. Ancora
    • 1
  1. 1.ENEA Dip. Ricerca e Sviluppo AgroindustrialiDiv. Ingegneria GeneticaRoma A.DItaly

Personalised recommendations