Transformation of Birds-Foot Trefoil (Lotus corniculatus)

  • Z. Tabaeizadeh
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)

Abstract

Legume-Rhizobium symbiosis leads to the de novo development of the root nodule, a highly specialized organ enabling the plant to reduce atmospheric nitrogen. Consequently, nitrogen fixation has attracted the attention of molecular biologists wishing to improve the efficiency of existing legume-bacteria interactions, and to transfer this capability to nonleguminous plants. In order to realize these goals a detailed understanding of the regulation and function of plant genes which are involved in the symbiotic nitrogen fixation process is necessary. This is only possible provided an efficient system for legume transformation is available. In this chapter, our work (Tabaeizadeh 1989) on genetic transformation of Lotus corniculatus (birds-foot trefoil), a perennial cross-pollinating tetraploid legume, which is extensively grown for pasture and hay in the north-central and northeastern United States and in eastern Canada is presented. The feeding value of birds-foot trefoil hay can equal that of alfalfa (Seaney and Henson 1970). We have developed a reproducible and extremely rapid system for the transformation of Lotus corniculatus using Agrobacterium rhizogenes.

Keywords

Sucrose Agar Germinate Beach Acetyl 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Z. Tabaeizadeh
    • 1
  1. 1.Department of Biological SciencesUniversity of Quebec in MontrealMontrealCanada

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