Transformation in Lettuce (Lactuca sativa L.)
Lettuce is an economically important green-yellow vegetable crop cultivated in many countries, but there is some difficulty in commercial production due to its susceptibility to high temperature and diseases in such humid climatic areas as Japan. Therefore, breeding of lettuce has focused on improving resistance to high temperature and diseases by conventional crossings between lettuce cultivars. Although wild lettuce species have resistance to downy mildew pathogen, etc., they cannot be readily crossed with lettuce. Recently, gene manipulation techniques such as cell fusion and transformation by electroporation or a binary vector system through Agrobacterium infection, etc., have been developed as useful tools for improving crops. Consequently, the introduction of genes rendering disease resistance and other useful traits to lettuce using biotechnology is expected in the near future.
KeywordsSalicylic Acid Chimeric Gene Salicylic Acid Treatment Lettuce Plant Agrobacterium Infection
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- Jefferson RA, Kavanagh TA, Bevan MV (1987) GUS fusions: jS-glucuronidase as a sensitive and versatile gene fusion markers in higher plants. EMBO J 6:3209–3212Google Scholar
- Michelmore R, Marsh E, Seely S, Landry B (1987) Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens. Plant Cell Rep 6:439–442Google Scholar
- Ohashi Y, Matsuoka M (1985) Synthesis of stress proteins in tobacco leaves. Plant Cell Physiol 26:473–480Google Scholar
- Roger SO, Bendich AJ (1988) Plant molecular biology manual A6:1–10. Kluwer, DordrechtGoogle Scholar