Transformation in Lettuce (Lactuca sativa L.)

  • S. Enomoto
  • Y. Ohashi
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)


Lettuce is an economically important green-yellow vegetable crop cultivated in many countries, but there is some difficulty in commercial production due to its susceptibility to high temperature and diseases in such humid climatic areas as Japan. Therefore, breeding of lettuce has focused on improving resistance to high temperature and diseases by conventional crossings between lettuce cultivars. Although wild lettuce species have resistance to downy mildew pathogen, etc., they cannot be readily crossed with lettuce. Recently, gene manipulation techniques such as cell fusion and transformation by electroporation or a binary vector system through Agrobacterium infection, etc., have been developed as useful tools for improving crops. Consequently, the introduction of genes rendering disease resistance and other useful traits to lettuce using biotechnology is expected in the near future.


Salicylic Acid Chimeric Gene Salicylic Acid Treatment Lettuce Plant Agrobacterium Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Chupeau MC, Bellini C, Guetche P, Maisonneve B, Vastra G, Chupeau Y (1989) Transgenic plants of lettuce species (Lactuca sativa L.) obtained through electroporation of protoplasts. Bio/Technology 7:503–508CrossRefGoogle Scholar
  2. Enomoto S, Maeda E (1989) A cyto-histological study on shoot differentiation and mesophyll morphology of the excised lettuce (Lactuca sativa L.) cotyledons as affected by light conditions during seedling growth. Jpn J Crop Sci 58:297–304CrossRefGoogle Scholar
  3. Enomoto S, Itoh H, Ohshima M, Ohashi Y (1990) Induced expression of chimeric gene construct in transgenic lettuce plants using tobacco pathogenesis-related protein gene promoter region. Plant Cell Rep 9:6–9CrossRefGoogle Scholar
  4. Jefferson RA, Kavanagh TA, Bevan MV (1987) GUS fusions: jS-glucuronidase as a sensitive and versatile gene fusion markers in higher plants. EMBO J 6:3209–3212Google Scholar
  5. Michelmore R, Marsh E, Seely S, Landry B (1987) Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens. Plant Cell Rep 6:439–442Google Scholar
  6. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  7. Ohashi Y, Matsuoka M (1985) Synthesis of stress proteins in tobacco leaves. Plant Cell Physiol 26:473–480Google Scholar
  8. Ohshima M, Matsuoka M, Yamamoto N, Tanaka Y, Kano-Murakami K, Ozeki Y, Kato A, Harada N, Ohashi Y (1987) Nucleotide sequence of the PR-1 gene of Nicotiana tabacum. FEBS Lett 225:243–246PubMedCrossRefGoogle Scholar
  9. Ohshima M, Itoh H, Matsuoka M, Murakami T, Ohashi Y (1990) Analysis of stress-induced or salicylic acid-induced expression of the pathogenesis-related la protein gene in transgenic tobacco. Plant Cell 2:95–106PubMedCrossRefGoogle Scholar
  10. Ooms G, Hooykaas PJJ, Moolenaar M, Schilperoort A (1981) Crown gall plant tumor of abnormal morophology induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions. Gene 14:33–50PubMedCrossRefGoogle Scholar
  11. Roger SO, Bendich AJ (1988) Plant molecular biology manual A6:1–10. Kluwer, DordrechtGoogle Scholar
  12. Van Loon LC (1985) Pathogenesis-related proteins. Plant Mol Biol 4:111–116CrossRefGoogle Scholar
  13. Webb DT, Torres LD (1984) Interaction of growth regulators, explant age, and culture environment controlling organogenesis from lettuce cotyledon in vitro. Can J Bot 62:586–590CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • S. Enomoto
  • Y. Ohashi
    • 1
  1. 1.Department of Cell BiologyNational Institute of Agrobiological ResourcesTsukuba, IbarakiJapan

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