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Transformation in Sugar Beet (Beta vulgaris L.)

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Plant Protoplasts and Genetic Engineering IV

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 23))

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Abstract

Sugar beet, Beta vulgaris L. (family Chenopodiaceae) is the major sucrose-producing crop grown in temperate zones, and contributes approximately 35% of the world’s supply, with the rest derived from sugarcane (Saccharum officinarum). The sucrose accumulates in the underground storage organ, or beet, and is extracted and purified after pulping. There are a number of targets for the genetic improvement of sugar beet (see Table 1), including improved harvesting and processing characteristics; the introduction of resistance to diseases, notably rhizomania and other viral diseases such as those caused by the beet yellows; and resistance to herbicides. However, in view of the fact that sugar beet is a biennial and highly heterozygous, being naturally cross-pollinated, conventional breeding is slow and difficult (Atanassov 1986).

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References

  • Atanassov AI (1986) Sugar Beet. In: Evans DA, Sharp WR, Ammirato PV (eds) Handbook of plant cell culture vol 4. MacMillan, New York pp 652–680

    Google Scholar 

  • Bates GW, Piastuch W, Riggs CD, Rabussay D (1988) Electroporation for DNA delivery to plant protoplasts. Plant Cell Tissue Organ Cult 12:213–218.

    Article  CAS  Google Scholar 

  • Bevan, MW (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 22:8711–8721

    Article  Google Scholar 

  • Bhat SR, Ford-Lloyd BV, Callow JA (1985) Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets. Plant Cell Rep 4:348–359

    Article  Google Scholar 

  • De Greef W, Jacobs M (1979) In vitro culture of the sugar beet - description of a cell line with high regeneration capacity. Plant Sci Lett 17:55–61

    Article  Google Scholar 

  • D’Halluin K, Bossut M, Bonne E, Mazur B, Leemans J, Botterman J (1992) Transformation of sugar beet (Beta vulgaris L.) and evaluation of herbicide resistance in transgenic plants. Bio/Technol 10:309–314

    Article  Google Scholar 

  • Freytag AH, Anand SC, Rao-Ardelli AP, Owens LD (1988) An improved method for adventitious shoot formation and callus induction in Beta vulgaris L. in vitro. Plant Cell Rep 7:30–34

    Article  Google Scholar 

  • Fromm ME, Taylor LP, Walbot V (1986) Stable transformation of maize after gene transfer by electroporation. Nature 319:791–793

    Article  PubMed  CAS  Google Scholar 

  • Gallois P, Lindsey K, Malone R, Kreis M, Jones MGK (1992) Gene rescue in plants by direct gene transfer of genomic DNA into protoplasts. Nucl Acids Res 20:3977–3982

    Article  PubMed  CAS  Google Scholar 

  • Gorman CM, Moffat LF, Howard BH (1982) Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol 2:1044–1051

    PubMed  CAS  Google Scholar 

  • Guerche P, Charbonnier M, Jouanin L, Tourneur C, Paszkowski J, Pelletier G (1987) Direct gene transfer by electroporation in Brassica napus. Plant Sci 52:111–116

    Google Scholar 

  • Hamill JD, Prescott A, Martin C (1987) Assessment of the efficiency of cotransformation of the T-DNA of disarmed binary vectors derived from Agrobacterium tumefaciens and the T-DNA of A. rhizogenes. Plant Mol Biol 9:573–584

    Google Scholar 

  • Harpster MH, Townsend JA, Jones JDG, Bedbrook J, Dunsmuir P (1988) Relative strengths of the 35S cauliflower mosaic virus, 1’, 2’, and nopaline synthase promoters in transformed tobacco, sugar beet and oilseed rape callus tissue. Mol Gen Genet 212:182–190

    Google Scholar 

  • Herman L, Jacobs A, Van Montagu M, Depicker A (1990) Plant chromosome/marker gene fusion assay for study of normal and truncated T-DNA integration events. Mol Gen Genet 224:248–256

    Google Scholar 

  • Hooker MP, Nabors MWZ (1977) Callus initiation, growth and organogenesis in sugar beet (Beta vulgaris L.) Z Pflanzenphysiologie 84:237–246

    Google Scholar 

  • Hussey G, Hepher A (1978) Clonal propagation of sugar beet plants and the formation of polyploids by tissue culture. Ann Bot 42:477–479

    Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    Google Scholar 

  • Jorgensen R, Snyder C, Jones JDG (1987) T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives. Mol Gen Genet 207:471–477

    Google Scholar 

  • Klee H, Horsch R, Rogers S (1987) Agrobacterium-medmtQd plant transformation and its further applications to plant biology. Annu Rev Plant Physiol 38:467–186

    Google Scholar 

  • Klein TM, Goff SA, Roth BA, Fromm, ME (1990) Applications of the particle gun in plant biology. In: Nijkamp HJJ, Van der Pias LHW, Van Aartrijk J (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht pp 56–66

    Google Scholar 

  • Krens FA, Jamar D, Rouwendal GJA, Hall RD (1990) Transfer of cytoplasm from new Beta CMS sources to sugar beet by asymmetric fusion. 1. Shoot regeneration from mesophyll protoplasts and characterization of regenerated plants. Theor Appl Genet 79:390–396

    Google Scholar 

  • Lawson C, Kaniewski W, Haley L, Rozman R, Newell C, Sanders P, Turner NE (1990) Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Biotechnology 8:127–134

    Google Scholar 

  • Lindner P, Neumann E, Rosenheck KJ (1977) Kinetics of permeability changes induced by electric impulses in chromaffin granules. Membr Biol 32:231–254

    Google Scholar 

  • Lindsey K (1992) Genetic manipulation of crop plants. J Biotechnol 26: 1–28

    Google Scholar 

  • Lindsey K, Gallois P (1990a) Direct gene transfer and gene rescue in sugar beet protoplasts. In: Sangwan RS, Sangwan-Norreel BS (eds) Impact of biotechnology in agriculture. Kluwer, Dordrecht, pp 355–380

    Google Scholar 

  • Lindsey K, Gallois P (1990b) Transformation of sugar beet (Beta vulgaris L) by Agrobacterium tumefaciens. J Exp Bot 41:529–536

    Google Scholar 

  • Lindsey K, Jones MGK (1987a) The permeability of electroporated cells and protoplasts of sugar beet. Planta 172:346–355

    Google Scholar 

  • Lindsey K, Jones MGK (1987b) Transient gene expression in electroporated protoplasts and intact cells of sugar beet. Plant Mol Biol 10:53–52

    Google Scholar 

  • Lindsey K, Jones MGK (1989) Stable transformation of sugar beet protoplasts by electroporation. Plant Cell Rep 8:71–74

    Google Scholar 

  • Lindsey K, Jones MGK (1990) Selection of transformed cells. In: Dix PJ. (ed) Plant cell line selection: procedures and applications. VCH, Weinheim, pp 317–339

    Google Scholar 

  • Lindsey K, Gallois P, Eady C (1991) Regeneration and transformation of sugar beet by Agrobacterium tumefaciens. In: Lindsey K (ed) Plant tissue culture manual: fundamentals and applications B7. Kluwer, Dordrecht, pp 1–13

    Google Scholar 

  • McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Leaf disc transformation of cultivated tomato (Lycopersicon esculentum) using Agrobacterium tumefaciens. Plant Cell Rep 5:81–84

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Google Scholar 

  • Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982) Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J 1:841–845

    Google Scholar 

  • Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3:2717–2722

    Google Scholar 

  • Paszkowski J, Baur M, Bogucki A, Potrykus I (1988) Gene targeting in plants. EMBO J 7:4021–4026

    Google Scholar 

  • Pröls M, Töpfer R, Schell J, Steinbiss H-H (1988) Transient gene expression in tobacco protoplasts: 1. Time course of CAT appearance. Plant Cell Rep 7:221–224

    Google Scholar 

  • Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer J J (1988) Genetically transformed maize plants from protoplasts. Science 240:204–207

    Google Scholar 

  • Ritchie GA, Short KC, Davey MR (1989) In vitro shoot regeneration form callus, leaf axis and petioles of sugar beet (Beta vulgaris L). J Exp Bot 40:277–283

    Google Scholar 

  • Rogozinska J, Goska M (1978) Induction of differentiation and plant formation in isolated sugar beet leaves. Bull Acad Pol Sei 26:343–345

    Google Scholar 

  • Saunders JW, Doley WP (1986) One step shoot regeneration from callus of whole plant leaf explants of sugar beet lines and a somaclonal variant for in vitro behaviour. J Plant Physiol 124:473–481

    Google Scholar 

  • Schauer AT (1988) Visualizing gene expression with luciferase fusions. Tibtech 6:23–27

    Google Scholar 

  • Shillito RD, Saul MW, Paszkowski J, Müller M, Potrykus I (1985) High efficiency direct gene transfer to plants. Bio/Technol 3:1099–1103

    Google Scholar 

  • Szabados L, Gaggero C (1985) Callus formation from protoplasts of a sugar beet cell suspension culture. Plant Cell Rep 4:195–198

    Google Scholar 

  • Tetu T, Sangwan RS, Sangwan-Norreel BS (1987) Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus. J Exp Bot 38:506–517

    Google Scholar 

  • van Geyt JPC, Jacobs M (1985) Suspension culture of sugarbeet (Beta vulgaris L.) Induction and habituation of dedifferentiated and self-regenerating cell lines. Plant Cell Rep 4:66–69

    Google Scholar 

  • Werr W, Lörz H (1986) Transient gene expression in a Gramineae cell line: a rapid procedure for studying plant promoters. Mol Gen Genet 202:471–475

    Article  CAS  Google Scholar 

  • Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47 (4): 189–194

    PubMed  CAS  Google Scholar 

  • Zhang HM, Yang H, Rech EL, Golds TJ, Davis AS, Mulligan BJ, Cocking EC, Davey MR (1988) Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts. Plant Cell Rep 7:379–384

    CAS  Google Scholar 

  • Zimmermann U (1986) Electrical breakdown, electropermeabilization and electrofusion. Rev Physiol Biochem Pharmacol 105:175–256

    Article  Google Scholar 

  • Zimmermann U, Vienken J, Pilwat G (1980) Development of drug carrier systems: electrical field induced effects in cell membranes. Bioelectrochem Bioenerg 7:553–574

    Article  CAS  Google Scholar 

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© 1993 Springer-Verlag Berlin Heidelberg

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Lindsey, K., Jones, M.G.K., Gallois, P., Eady, C. (1993). Transformation in Sugar Beet (Beta vulgaris L.). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering IV. Biotechnology in Agriculture and Forestry, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78037-0_12

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  • DOI: https://doi.org/10.1007/978-3-642-78037-0_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78039-4

  • Online ISBN: 978-3-642-78037-0

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