Partial Characterization of the Secretory Products of the Subcommissural Organ
Histochemically, the secretory material of the subcommissural organ (SCO) has been characterized as a polysaccharide—protein complex (Oksche 1962; Naumann 1968; Diederen 1970); most probably it represents N-linked complex-type glycoproteins (Meiniel and Meiniel 1985; Meiniel et al. 1986, 1988; Rodríguez et al. 1986, 1987, 1990; Herrera and Rodríguez 1990). The immunocytochemical study of the bovine SCO using antibodies raised against bovine Reissner’s fiber (RF) showed that the secretory material present in the rough endoplasmic reticulum (RER), secretory granules, and RF is strongly immunoreactive (Hein 1988; Nualart et al. 1991). Concanavalin A (ConA, affinity = mannose, glucose)-binding sites have been only found in the RER, whereas Limax flavus agglutinin (LFA, affinity = sialic acid) bound to the secretory material is present in the secretory granules and RF (Nualart et al. 1991).
KeywordsCellulose Carbohydrate Polysaccharide Electrophoresis Polypeptide
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