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Partial Characterization of the Secretory Products of the Subcommissural Organ

  • S. Hein
  • F. Nualart
  • E. M. Rodríguez
  • A. Oksche

Abstract

Histochemically, the secretory material of the subcommissural organ (SCO) has been characterized as a polysaccharide—protein complex (Oksche 1962; Naumann 1968; Diederen 1970); most probably it represents N-linked complex-type glycoproteins (Meiniel and Meiniel 1985; Meiniel et al. 1986, 1988; Rodríguez et al. 1986, 1987, 1990; Herrera and Rodríguez 1990). The immunocytochemical study of the bovine SCO using antibodies raised against bovine Reissner’s fiber (RF) showed that the secretory material present in the rough endoplasmic reticulum (RER), secretory granules, and RF is strongly immunoreactive (Hein 1988; Nualart et al. 1991). Concanavalin A (ConA, affinity = mannose, glucose)-binding sites have been only found in the RER, whereas Limax flavus agglutinin (LFA, affinity = sialic acid) bound to the secretory material is present in the secretory granules and RF (Nualart et al. 1991).

Keywords

Secretory Granule Rough Endoplasmic Reticulum Secretory Product Coomassie Blue Apparent Mass 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • S. Hein
  • F. Nualart
  • E. M. Rodríguez
  • A. Oksche

There are no affiliations available

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